Changes of DNA Methylation Pattern in Metabolic Pathways Induced by High-Carbohydrate Diet Contribute to Hyperglycemia and Fat Deposition in Grass Carp (Ctenopharyngodon idellus)

Cai, Wenjing; Liang, Xu‐Fang; Yuan, Xiaochen; Li, Aixuan; He, Shan

doi:10.3389/fendo.2020.00398

Cited by 15 publications

(12 citation statements)

References 66 publications

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“…Our results showed that the genome-wide methylation patterns were similar between two groups. Approximately 9% of all total cytosines and 80% of CG were methylated and only a small proportions of methylation at non-CpG methylation (CHG and CHH) was observed within all regions, which is consistent with previous study (Cai et al, 2020). The high level of DNA methylation in CG content was a specific characteristic of animals.…”

Section: Dna Methylation Profiles In Domesticated and Wild Grass Carpsupporting

confidence: 92%

“…A total of 20 wild sub-adult grass carp at the approximately 18-month old determined by annulus characteristics of scale (Cai et al, 2020), were captured using traps and gill nets along the Xijiang river in Zhaoqing City, Guangdong Province, China, within a 1000 m radius from the following coordinates (latitude: 23.1034.8 °N; longitude: 112.4554.0 °E). Subsequently, the fish were transported to breeding base and randomly allocated into two ponds (1.5 m × 1.5 m × 1 m), approximately 250 bp, followed by the blunt-ending, dA addition to 3'-end, finally, adaptor ligation(in this case of methylated adaptors to protect from bisulfite conversion), essentially according to the manufacturer's instructions.…”

Section: Domesticated Trialmentioning

confidence: 99%

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Genome-wide analysis of DNA methylation reveals selection signatures of grass carp during the domestication

Zhang

et al. 2021

Preprint

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With the rapid development of aquaculture, more and more fish species from wild environments are artificially domesticated and cultured. In the process of domestication, the fish develop some adaptations and phenotypic traits, namely selection signatures. However, it is still unclear about the biological process underlying these selection signatures. Here, we used grass carp (Ctenopharyngodon idellus), an aquaculture fish with the largest production worldwide, to detect its selection signatures and investigate the roles of DNA methylation in the emergence of selection signatures during domestication based on whole-genome bisulfite sequencing technology. Our results showed that domesticated grass carp demonstrated four selection signatures, including growth and metabolism, immunity, foraging and learning behaviors, and 38 candidate genes were associated with these traits. 16 of candidate genes, such as IGF-1, GK, GYS1, etc., were found to play major roles in the growth and metabolism. Immunity signature was related to 11 of candidate genes, including MHCI, MHCII, C1QA, etc. The GRM1, TAS1R1 and TAS1R3 genes were essential for the adaptation of domesticated grass carp to commercial feed in artificial rearing condition. The C-FOS, POMC and CBP genes might be responsible for the acquisition of novel feeding habits and contribute to faster growth indirectly by enhancing food intake. These findings would provide new insights to expand our understanding on the role of DNA methylation in shaping physiological phenotypes in fish, and also contribute to efficient breeding of aquaculture stocks and restocking programs.

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Section: Dna Methylation Profiles In Domesticated and Wild Grass Carpsupporting

confidence: 92%

Section: Domesticated Trialmentioning

confidence: 99%

Genome-wide analysis of DNA methylation reveals selection signatures of grass carp during the domestication

Zhang

et al. 2021

Preprint

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“…However, the deferentially methylated genes in Ctenopharyngodon idellus with a high carbohydrate diet were enriched in pathways involved in glucose metabolism, insulin signaling, and lipid metabolism. Most of these obesity and T2DM-involved methylation changes were also observed in mammals (87). Moreover, folic acid (synthetic form of folate) is mainly involved in the methylation processes acting as a methyl donor for the synthetic process of S-adenosylmethionine (108).…”

Section: Diet and Epigenetic Interplay In T1dm Pathogenesismentioning

confidence: 99%

“…The diet-epigenetic axis is also implicated in pathways related to glucose metabolism and insulin signaling, as demonstrated in animal models. Whole genome DNA methylation analysis of the grass carp, Ctenopharyngodon idellus, revealed no significant changes in methylation level of metabolic genes between different nutritional conditions (high carbohydrate diet/ normal diet) ( 87 ). However, the deferentially methylated genes in Ctenopharyngodon idellus with a high carbohydrate diet were enriched in pathways involved in glucose metabolism, insulin signaling, and lipid metabolism.…”

Section: Diet and Epigenetic Interplay In T1dm Pathogenesismentioning

confidence: 99%

The Interplay Between Diet and the Epigenome in the Pathogenesis of Type-1 Diabetes

et al. 2021

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The autoimmune disease, Type 1 Diabetes Mellitus (T1DM), results in the destruction of pancreatic β-cells, and the International Diabetes Federation reports that its incidence is increasing worldwide. T1DM is a complex disease due to the interaction between genetic and environmental factors. Certain dietary patterns and nutrients are known to cause epigenetic modifications in physiological conditions and diseases. However, the interplay between diet and epigenetics is not yet well-understood in the context of T1DM. Several studies have described epigenetic mechanisms involved in the autoimmune reactions that destroy the β-cells, but few explored diet components as potential triggers for epigenetic modifications. Clarifying the link between diet and epigenome can provide new insights into the pathogenesis of T1DM, potentially leading to new diagnostic and therapeutic approaches. In this mini review, we shed light on the influence of the diet-epigenome axis on the pathophysiology of T1DM.

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“…DNMT inhibitors function by blocking the key methylation enzymes such as DNMT1, DNMT3a and DNMT3b, which subsequently blocks DNA methylation. For instance, 5-azacitidine, a structural analogue of cytosine, when incorporated into DNA, is methylated by covalently bound DNMT3a, eventually inhibiting the enzyme activity [ 104 ]. A study by You et al [ 105 ] utilizing insulin-resistant 3T3-L1 adipocytes identified an increased percentage of insulin-stimulated glucose uptake upon treatment with 5-azacitidine [ 105 ].…”

Section: Epigenetic Strategies For Mets Prevention/reversalmentioning

confidence: 99%

Epigenetics, microRNA and Metabolic Syndrome: A Comprehensive Review

Ramzan

Vickers

Mithen

2021

IJMS

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Epigenetics refers to the DNA chemistry changes that result in the modification of gene transcription and translation independently of the underlying DNA coding sequence. Epigenetic modifications are reported to involve various molecular mechanisms, including classical epigenetic changes affecting DNA methylation and histone modifications and small RNA-mediated processes, particularly that of microRNAs. Epigenetic changes are reversible and are closely interconnected. They are recognised to play a critical role as mediators of gene regulation, and any alteration in these mechanisms has been identified to mediate various pathophysiological conditions. Moreover, genetic predisposition and environmental factors, including dietary alterations, lifestyle or metabolic status, are identified to interact with the human epigenome, highlighting the importance of epigenetic factors as underlying processes in the aetiology of various diseases such as MetS. This review will reflect on how both the classical and microRNA-regulated epigenetic changes are associated with the pathophysiology of metabolic syndrome. We will then focus on the various aspects of epigenetic-based strategies used to modify MetS outcomes, including epigenetic diet, epigenetic drugs, epigenome editing tools and miRNA-based therapies.

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Changes of DNA Methylation Pattern in Metabolic Pathways Induced by High-Carbohydrate Diet Contribute to Hyperglycemia and Fat Deposition in Grass Carp (Ctenopharyngodon idellus)

Cited by 15 publications

References 66 publications

Genome-wide analysis of DNA methylation reveals selection signatures of grass carp during the domestication

Genome-wide analysis of DNA methylation reveals selection signatures of grass carp during the domestication

The Interplay Between Diet and the Epigenome in the Pathogenesis of Type-1 Diabetes

Epigenetics, microRNA and Metabolic Syndrome: A Comprehensive Review

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