Changes of chromatin and cell membranes in exfoliated human buccal epithelium cells exposed to non-ionizing and ionizing electromagnetic fields

Kuznetsov, K.A.; Shckorbatov, Y. G.; Kolchigin, N. N.; Ніколов, О. Т.

doi:10.1109/uwbusis.2016.7724179

Cited by 2 publications

(3 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For cells of donor A 1 Gy dose induced statistically significant increase in HGQ. For cells of both donors the value of HGQ at 2-5 Gy was similar, forming so-called "plateau" which is described earlier [25]. The increase in HGQ in cells of both donors was about 25% (Fig.…”

Section: Resultssupporting

confidence: 77%

See 1 more Smart Citation

Response of exfoliated human buccal epithelium cells to combined gamma radiation, microwaves, and magnetic field exposure estimated by changes in chromatin condensation and cell membrane permeability

Kuznetsov

Ніколов

Shckorbatov

2016

Biophysical Bulletin

View full text Add to dashboard Cite

Modulation of the biological effects produced by ionizing radiation (IR) using microwave and magnetic fields has important theoretical and practical applications. Response of human buccal epithelium cells to different physical agents (single and combined exposure to 0.5–5 Gy γ-radiation (60Co); microwaves with the frequency of 36.64 GHz and power densities of 0.1 and 1 W/m2, and static magnetic field with the intensity of 25 mT) has been investigated. The stress response of the cells was evaluated by counting heterochromatin granules quantity (HGQ) in the cell nuclei stained with orcein. Membrane permeability was assessed by the percentage of cells stained with indigocarmine (cells with damaged membrane). The increase of heterochromatin granules quantity (HGQ), i.e. chromatin condensation was detected at the doses of 2 Gy and higher. Changes in the cell membrane permeability to indigocarmine expressed the threshold effect. Membrane permeability reached the threshold at the doses of 2–3 Gy for the cells of different donors and did not change with the increase of the dose of γ-radiation. Cells obtained from different donors revealed some individual peculiarities in their reaction to γ-radiation. The static magnetic field and microwaves applied before or after γ-radiation decreased its impact, as revealed by means of HGQ assessment.

show abstract

Section: Resultssupporting

confidence: 77%

“…Combined exposures were conducted by using the magnetic field and microwaves before and after γirradiation with 15 min interval between exposures. These exposure combinations proved their efficiency in modification of γ-radiation biological effects in human cells [25].…”

Section: Methodsmentioning

confidence: 87%

Response of exfoliated human buccal epithelium cells to combined gamma radiation, microwaves, and magnetic field exposure estimated by changes in chromatin condensation and cell membrane permeability

Kuznetsov

Ніколов

Shckorbatov

2016

Biophysical Bulletin

View full text Add to dashboard Cite

show abstract

“…У нервово-м'язових закінченнях зменшується кількість синаптичних пухирців та мітохондрій, які мають просвітлений матрикс і зруйновані кристи. При цьому нейролемоцити таких нервових волокон містять дрібні гетерохроматизовані ядра, що, на думку Kuznetsov et al (2016), свідчить про глибоке порушення мітотичної та білоксинтезувальної активності клітини.…”

Section: обговоренняunclassified

Left ventricular diastolic function in patients with obstructive sleep apnoea syndrome and obesity

Luchynskyi

Luchinskyi

Shcherba

et al. 2017

Regul. Mech. Biosyst.

View full text Add to dashboard Cite

Obstructive sleep apnea syndrome (OSAS) is a chronic disease characterized by recurrent episodes of complete or partial obstruction of the upper airways during sleep and results in sleep fragmentation, night hypoxemia, and daytime sleepiness. The aim of this study was to investigate the state of left ventricular diastolic function in patients with first diagnosed COAS and obesity and to define the determinants of diastolic dysfunction in this category of patients. There were 46 patients (33 men and 13 women) with OSAS and obesity and 52 patients with obesity without OSAS enrolled in the study. The control group included 22 practically healthy individuals. Each patient underwent assessment of body mass index (BMI), 24-hour ambulatory blood pressure monitoring, cardiorespiratory monitoring, transthoracic echocardiography. We found that patients with OSAS and obesity had significant impairment of diastolic function in comparison with patients with obesity without OSAS, which manifested itself in decreasing of E/A ratio, increasing deceleration time and increasing isovolumic relaxation time. Increasing body mass index and increasing level of hypoxia lead to impairment of diastolic dysfunction. Diastolic dysfunction was diagnosed in 4 obese patients without OSAS and in 17 patients with OSAS and obesity. All patients with diastolic dysfunction had second or third stage obesity and severe OSAS. Left ventricular hypertrophy was diagnosed in 9 patients with OSAS and obesity. Patients with concentric hypertrophy had significantly lower E/A in comparison with patients without hypertrophy or with concentric remodeling. We found that the deceleration time has positive correlation with body mass index and desaturation index and negative correlation with min SaO2. Isovolumic relaxation time correlated only with desaturation index. According to the results of univariable regression analysis, five variables were allocated: body mass index, age, desaturation index, index apnea-hypopnea and left ventricular mass index. The multivariate analyses, after adjustment for age and sex and stepwise regression, showed that only the desaturation index was an independent predictor of left ventricular diastolic dysfunction in patients with OSAS and obesity. Obstructive sleep apnea syndrome combined with obesity leads to a deterioration of the diastolic function of the left ventricle even in patients without cardio-vascular diseases. The state of the diastolic function of the left ventricule worsens with an increase in the severity of hypoxic disorders in the syndrome of obstructive sleep apnea and with the degree of obesity. In multivariate regression analysis, the desaturation index was a predictor of left ventricular diastolic dysfunction in patients with obstructive sleep apnea syndrome and obesity.

show abstract

Changes of chromatin and cell membranes in exfoliated human buccal epithelium cells exposed to non-ionizing and ionizing electromagnetic fields

Cited by 2 publications

References 6 publications

Response of exfoliated human buccal epithelium cells to combined gamma radiation, microwaves, and magnetic field exposure estimated by changes in chromatin condensation and cell membrane permeability

Response of exfoliated human buccal epithelium cells to combined gamma radiation, microwaves, and magnetic field exposure estimated by changes in chromatin condensation and cell membrane permeability

Left ventricular diastolic function in patients with obstructive sleep apnoea syndrome and obesity

Contact Info

Product

Resources

About