Changes of cecal microflora in chickens following Eimeria tenella challenge and regulating effect of coated sodium butyrate

Zhou, Zhixiong; Nie, Kun; Huang, Qingzhou; Li, Kai; Sun, Yingying; Zhou, Rongqiong; Wang, Zhiying; Hu, Shijun

doi:10.1016/j.exppara.2017.04.007

Cited by 42 publications

(33 citation statements)

References 41 publications

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“…Literature indicates that supplementing diets of broilers with butyrate influences cecal microbiota composition in a way that is beneficial for the health and growth performance when the microbiota is disturbed by for example an enteric disease or nutritional challenge [36][37][38]. We also observed that supplementing butyrate in a wax-based carrier can reduce the Salmonella count, which very likely explains the observed decrease of Enterobacteriaceae.…”

Section: Discussionsupporting

confidence: 56%

Effect of in feed administration of different butyrate formulations on Salmonella Enteritidis colonization and cecal microbiota in broilers

Onrust

Baeyen²,

Haesebrouck

et al. 2020

Vet Res

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Butyrate has been used extensively as a feed additive to improve gut health and to decrease Salmonella colonization in poultry. Salmonella mainly colonizes the ceca so butyrate concentrations should be increased in this gut segment. Discrepancies on the effects of butyrate on Salmonella colonization, described in the scientific literature, could thus be due to butyrate release location effects. In this study, newly developed butyrate formulations were evaluated for their effect on cecal butyrate concentrations and on colonization by Salmonella Enteritidis. In a first trial, broilers were randomly allocated to 7 dietary treatment groups with formulations based on different approaches to modify the butyrate release profile: release from wax matrices based on diffusion/erosion; micropellets supposedly release butyrate around pH 7 in the colon; tributyrin is based on the hydrolysis of esters in the small intestine. Fat-protected butyrate was included as a reference, because of its known effect on reduction of Salmonella colonization. Four days after infection, the number of cfu Salmonella per g cecal content and spleen were determined. Butyrate formulations in a wax matrix significantly reduced the Salmonella colonization in cecal content. In a second trial, wax and fat-protected butyrate treatments were replicated and results from the first trial were confirmed. Compared to the control group a higher proportion of butyrate concentration was observed in ceca for those groups with reduced Salmonella colonization. This was associated with a beneficial shift in the cecal microbiota. In conclusion, formulations that increase cecal butyrate concentrations are superior in protecting against Salmonella Enteritidis colonization.

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Section: Discussionsupporting

confidence: 56%

Effect of in feed administration of different butyrate formulations on Salmonella Enteritidis colonization and cecal microbiota in broilers

Onrust

Baeyen²,

Haesebrouck

et al. 2020

Vet Res

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“…As a feed additive, SB is prone to alter the structure of gut microbiome [3,4,14]. Our data provides evidence that SB could change the intestinal microbial flora of broilers ( Supplementary Figure 6).…”

Section: Discussionmentioning

confidence: 59%

“…Therefore, there may exist differences in butyrate's regulation of lipid metabolism between chickens and that in mammals. Some butyrate derived feed additives, such as SB, calcium butyrate, and butyrate glycerides have been used in poultry production [14][15][16][17][18]. Although some studies have identified a reduced abdominal fat ratio and an altered hepatic genes expression with dietary supplementation of butyrate derivatives in broiler chickens [17,18], the underlying mechanisms remain to be fully understood.…”

Section: Introductionmentioning

confidence: 99%

Low and high concentrations of butyrate regulate fat accumulation in chicken adipocytes via different mechanisms

et al. 2020

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The present study investigated the effects of varying concentrations of sodium butyrate (SB) on fat accumulation and cell proliferation in chicken adipocytes. High and low serial concentrations of SB used significantly reduced adipocytic fat accumulation. However, they were observed to exhibit differences in cell morphology and distinctions in lipogenic genes expression profiles. At lower concentration (0.01 mM), fat accumulation was decreased with an associated downregulation in the expression of lipogenic genes, which was mediated by free fatty acid receptors (FFARs). Contarily, at higher concentration (1 mM), the fat droplets laden in adipocytes were enlarged, and this was accompanied with activation of lipogenic genes expression. However, the total accumulated fat was also decreased largely due to reduction in cell numbers, which was partially attributable to the reduction in histone deacetylase (HDAC) activity. Animal experiments further indicated that dietary supplementation of lower dose coated SB (0.1% wt/wt) inhibited fat deposition in livers and abdominal fat tissues of broilers, suggesting the potential application of sodium butyrate as feed additive in the regulation of fat deposition.

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“…Nutritional strategies targeting the modulation of the microbiota before and after a challenge have the potential to improve development and regeneration of the injured intestinal mucosa, digestive physiology, immune system and inflammation (Kogut, 2017). Few studies have investigated the effect of Eimeria infection on the microbial community richness (Stanley et al, 2012;Macdonald et al, 2017;Zhou et al, 2017), and have observed that challenge itself does not have a strong effect on this parameter. Challenge with Eimeria plus C. perfringens, however, drastically changes the diversity and composition of the cecal microbiota (Stanley et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

“…The increased mucus production that occurs due to the Eimeria infection (Collier et al, 2008), and leakage of plasma proteins into the intestinal lumen (Prescott et al, 2016) increase the proliferation of Clostridium perfringens, the causative agent of NE (Prescott et al, 2016). Besides increasing the susceptibility to NE, coccidiosis also leads to changes in the overall structure of the intestinal microbiota (Stanley et al, 2014;Wu et al, 2014;Zhou et al, 2017). Wu et al (2014) demonstrated a reduction in the cecal microbial diversity following Eimeria infection, and reduction of many members of the family Ruminococcaceae.…”

Section: Introductionmentioning

confidence: 99%

Supplementation of Protected Sodium Butyrate Alone or in Combination With Essential Oils Modulated the Cecal Microbiota of Broiler Chickens Challenged With Coccidia and Clostridium perfringens

Bortoluzzi

Rothrock

Vieira

et al. 2018

Front. Sustain. Food Syst.

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The objective of this study was to determine the effects of protected sodium butyrate (SB), and protected sodium butyrate plus essential oils (carvacrol and ginger; SBEO) on the cecal microbiota of broilers challenged with Eimeria maxima and Clostridium perfringens. Birds were assigned to 4 treatments (8 replicates pens of 58 birds/pen): unchallenged control; challenged control; challenged and supplemented with SB; challenged and supplemented with SBEO. On d 13, challenged birds were orally inoculated with ∼5,000 Eimeria maxima oocysts. On d 18-19, the same birds were exposed to Clostridium perfringens via drinking water (∼8 log CFU/ml). Cecal excreta was collected at d 12, 18, 21, and 28 for microbiota analysis through 16s rRNA sequencing using Illumina MiSeq platform and analyzed using QIIME v. 1.9.1 The cecal microbiota was analyzed over time within each experimental group. The inclusion of SB alone or in combination with EO contributed to larger variations in the cecal microbiota over time than the unsupplemented treatments, as shown by the diversity indices. The community structure and abundance of the cecal microbiota were significantly different across ages, especially in the groups supplemented with SB and SBEO. As shown in the PCoA analysis, the supplementation of SB led to a more stable microbial community and lower between-sample variability over time. In the unchallenged control birds, Ruminococcus decreased (p = 0.006), whereas Bacteroides and Clostridiales increased (p ≤ 0.02) as the birds aged. In the challenged control group, however, the frequency of Coprococcus and Blautia decreased as birds aged (p ≤ 0.01), and, Clostridiales did not increase. Supplementation of SB, but not SBEO, increased the frequency of Lactobacillus (p = 0.01) on d 12 compared to d 18 and d 28, and prevented the reduction in the frequency of Blautia as the birds aged. Nevertheless, supplementation Bortoluzzi et al. Necrotic Enteritis and Nutrition for Broilers of SB and SBEO contributed to unique changes in the predicted functions of the cecal microbiota over time, which was not observed in the unsupplemented birds. SB and SBEO modulated the diversity, composition, and predictive function of the cecal microbiota which may have lowered the negative impact of necrotic enteritis (NE).

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Changes of cecal microflora in chickens following Eimeria tenella challenge and regulating effect of coated sodium butyrate

Cited by 42 publications

References 41 publications

Effect of in feed administration of different butyrate formulations on Salmonella Enteritidis colonization and cecal microbiota in broilers

Effect of in feed administration of different butyrate formulations on Salmonella Enteritidis colonization and cecal microbiota in broilers

Low and high concentrations of butyrate regulate fat accumulation in chicken adipocytes via different mechanisms

Supplementation of Protected Sodium Butyrate Alone or in Combination With Essential Oils Modulated the Cecal Microbiota of Broiler Chickens Challenged With Coccidia and Clostridium perfringens

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