Changes induced by gamma ray irradiation on biomass production and secondary metabolites accumulation in Hypericum triquetrifolium Turra callus cultures

Azeez, Hoshyar A.; Ibrahim, Kadhim M.; Pop, Rodica; Pamfil, Doru; Hârța, Monica; Bobiş, Otilia

doi:10.1016/j.indcrop.2017.06.040

Cited by 33 publications

(20 citation statements)

References 48 publications

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“…Regarding the HPLC analysis, 3-Hydroxy-4-methoxycinnamic acid, was appeared to be the main phenolic acid in callus infusion, this result is consistent with the findings of Arafa et al (2015) and Azeez et al (2017). These authors have reported that it is the major compound of callus produced under in vitro conditions.…”

Section: Discussionsupporting

confidence: 85%

Evaluation of Acute Toxicity and Antiinflammatory Activity of Callus Extracts of Pulicaria Incisa (Lam.) Dc.

Rouane¹,

Chabane²,

Arab³

2019

Appl. Ecol. Env. Res.

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This study was aimed to investigate the effect of bioactive molecules of callus infusion of Pulicaria incisa (Lam.) DC, by the evaluation of anti-inflammatory activity and acute toxicity. 70% was the higher rate of callus obtained from young capitula cultivated on Murashige and Skoog (1962) (MS) solid medium containing 2.4D 4.10 -5 M and KIN 5.10 -6 M for 4 months. A Phytochemical screening based on colorimetric reactions was performed on the callus infusion material. Unlike the absence of primary components (starch, reducing sugars), secondary metabolites such as mucilage, saponins, flavonoids and anthocyanins were revealed. No sign of toxicity was observed by the application of 0.25 to 5 g/kg doses in this investigation, although, a significant anti-inflammatory response was revealed at 5 g/kg compared to that of Diclofenac ® . The chromatographic analysis (HPLC) detected the presence of 3-hydroxy-4methoxycinnamic acid the main phenolic acid produced by callus, which could be responsible of the positive anti-inflammatory reaction. These findings suggest that the callus could be an alternative source of bioactive metabolites useful in the human health as an anti-inflammatory source.

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Section: Discussionsupporting

confidence: 85%

Evaluation of Acute Toxicity and Antiinflammatory Activity of Callus Extracts of Pulicaria Incisa (Lam.) Dc.

Rouane¹,

Chabane²,

Arab³

2019

Appl. Ecol. Env. Res.

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“…It was reported that thidiazuron in combination with other plant growth regulators induced callus [38], somatic embryos [39], and shoot regeneration from friable callus [40]. Hence, in this study we have used TDZ in combination with BAP for the indirection regeneration of R. tetraphylla .…”

Section: Resultsmentioning

confidence: 99%

Indirect Regeneration and Assessment of Genetic Fidelity of Acclimated Plantlets by SCoT, ISSR, and RAPD Markers in Rauwolfia tetraphylla L.: An Endangered Medicinal Plant

Rohela

Jogam

Bylla

et al. 2019

BioMed Research International

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Rauwolfia tetraphylla L. is an important medicinal plant species which is well known for its pharmaceutically important alkaloids. In the present study, we are reporting about its conservation by in vitro clonal multiplication through the standardized protocol of indirect regeneration by using leaf and stem based callus and assessment of genetic fidelity of acclimated plantlets by start codon targeted (SCoT), inter simple sequence repeats (ISSR), and randomly amplified polymorphic DNA (RAPD) marker based analysis. Initially friable callus was induced in maximum amounts (378.7, 323.8, and 412.8 in mg) from leaf, root, and stem explants on Murashige and Skoog (MS) media supplemented with 5.0 mg/L, 3.0 mg/L of 2,4-dichlorophenoxyacetic acid (2,4-D) and 5.0 mg/L of naphthalene acetic acid (NAA), respectively. Shoot regeneration with the maximum number of shoot buds (25 and 20) was obtained from leaf and stem calluses on MS media supplemented with TDZ (0.25 mg/L) + BAP (2 mg/L). The regenerated shoots were rooted successfully with maximum rooting percentage of 98.0 on full strength MS media amended with IAA (1.0 mg/L) and IBA (1.0 mg/L). The regenerated plantlets were hardened using 2:1 ratio of sterile garden soil and sand, followed by acclimatization in field conditions with 86% of survival. SCoT, ISSR, and RAPD primers based polymerase chain reaction (PCR) analysis was carried out to check possible genetic variations in micro propagated plants in comparison with mother plant. Among the ten SCoT (S), ISSR (R), and RAPD (OPA) primers used, S2, R10, and OPA3 has given good amplification with scorable DNA bands. The results revealed that the regenerated plants did not have any polymorphism with mother plant. Hence, the in vitro regenerated R. tetraphylla plantlets were confirmed as true-to-type.

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“…HPLC analysis was carried out using a Shimadzu binary gradient unit LC-10AD VP (Shimadzu Instruments, Kyoto, Japan) system, equipped with a SCL10AVP system controller, SPP-M20A Prominence Diode Array Detector, LC-10ADSP binary pumps, CTO-10AVP column oven and SIL-10AF autosampler [55]. Separation was performed on a Teknokroma Mediterranean Sea 18 15 × 0.46 cm, i.d.…”

Section: Analytical Hplc-pda Determinationmentioning

confidence: 99%

Micropropagation, Genetic Fidelity and Phenolic Compound Production of Rheum rhabarbarum L.

Clapa

Borsai²,

Hârța

et al. 2020

Plants

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An efficient micropropagation protocol for Rheum rhabarbarum L. was developed in this study. The in vitro rhubarb plants obtained in the multiplication stage (proliferation rate: 5.0 ± 0.5) were rooted in vitro (96% rooting percentage) and acclimatized ex vitro in floating perlite, with 90% acclimatization percentage. To assess the genetic fidelity between the mother plant and in vitro propagated plants, sequence-related amplified polymorphism (SRAP) markers were used. All banding profiles from the micropropagated plants were monomorphic and similar to those of the mother plant indicating 100% similarity. Regarding the polyphenolic profile, gallic, protocatechuic, p-hydroxybenzoic, vanillic, chlorogenic, caffeic, syringic, p-coumaric and ferulic acid were present in different amounts (2.3–2690.3 μg g−1 dry plant), according to the extracted matrix. Aglicons and glycosides of different classes of flavonoids were also identified. The rhizome extracts (both from in vitro and field grown plants) contained resveratrol, a stilbene compound with high antioxidant properties, ranging between 229.4 to 371.7 μg g−1 plant. Our results suggest that in vitro propagation of Rheum rhabarbarum L. represents a reliable alternative to obtain a large number of true-to-type planting material with high bioactive compound content of this valuable nutritional and medicinal species.

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Changes induced by gamma ray irradiation on biomass production and secondary metabolites accumulation in Hypericum triquetrifolium Turra callus cultures

Cited by 33 publications

References 48 publications

Evaluation of Acute Toxicity and Antiinflammatory Activity of Callus Extracts of Pulicaria Incisa (Lam.) Dc.

Evaluation of Acute Toxicity and Antiinflammatory Activity of Callus Extracts of Pulicaria Incisa (Lam.) Dc.

Indirect Regeneration and Assessment of Genetic Fidelity of Acclimated Plantlets by SCoT, ISSR, and RAPD Markers in Rauwolfia tetraphylla L.: An Endangered Medicinal Plant

Micropropagation, Genetic Fidelity and Phenolic Compound Production of Rheum rhabarbarum L.

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