Changes in Water Content, Collagen Degradation, Collagen Content, and Concentration in Repeated Biopsies of the Cervix of Pregnant Cows

Breeveld-Dwarkasing, V.N.A.; Koppele, J.M. te; Bank, Ruud A.; Weijden, G.C. van der; Taverne, M.A.M.; Dissel‐Emiliani, F. M. F. Van

doi:10.1095/biolreprod.102.012534

Cited by 39 publications

(27 citation statements)

References 30 publications

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“…Also others described a cervical diameter of 20 cm at 1.5 h after spontaneous parturition [25]. Collagen breakdown is the main contributor to the increased softness of the cervix around parturition [2][3][4], but it would not be expected that in such a short time new collagen fibrils would have lead to stiffening of the once-flaccid tissue and contributed to this prompt recovery of the diameter. Besides collagen, the cervical stromal layer contains much smooth muscular tissue [24] which contracts under influence of oxytocin in vitro.…”

Section: Diameter Of the Cervixmentioning

confidence: 99%

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Cervical diameter in relation to uterine and cervical EMG activity in early postpartum dairy cows with retained placentas after PGF2alpha induced calving

et al. 2007

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The cervix must regain its normal diameter after parturition. Until now, little has been known about the pattern of cervical closure and the possible influences of myometrial and cervical contractions in this process. We continuously measured the cervical diameter with ultrasound cervimetry during the first 48 h after calving in six cows with retained fetal membranes, while uterine (n = 6) and cervical outer muscular layer (n = 4) electromyographic (EMG) activity was measured with bipolar EMG electrodes.We found that the cervical diameter which was 6.2 cm (AE0.7) at 1.4 h after calving, initially increased to 9.0 cm (AE1.0) during the first 14.8 h (AE2.8) postpartum. After this time, the diameter decreased gradually to 5.3 cm (AE1.0) at 48 h after calving. The overall EMG activity after parturition decreased by 59% (AE6) and 35% (AE17) for the uterus and cervix, respectively. The decrease in EMG activity was due to a 50% (AE7) decrease in EMG amplitudes of the myometrium; the EMG amplitudes of the cervix decreased by only 8% (AE21) (P > 0.05). At the same time in the cervix, burst frequency decreased by 69% (AE17), while the decrease in burst frequency of the myometrium was only 11% (AE5) (P > 0.05).Uterine myometrial and cervical EMG activity after parturition showed burst patterns. These contractions of the uterus and cervix were accompanied by and correlated with transient dilatations of the caudal cervix. This could have functional relevance in the evacuation of the uterus. #

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Section: Diameter Of the Cervixmentioning

confidence: 99%

“…Shortly before and during parturition, the connective tissue of the cervix is remodelled [2][3][4] and loses its strength [5] which results in a softened cervix that is able to dilate and accommodate the fetus when the uterus contracts [6]. The cervix is an important barrier against the invasion of bacteria in the uterine cavity [7,8].…”

Section: Introductionmentioning

confidence: 99%

Cervical diameter in relation to uterine and cervical EMG activity in early postpartum dairy cows with retained placentas after PGF2alpha induced calving

et al. 2007

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“…Murine and bovine models suggest two or three layers of collagen in the cervix: a wide central circumferential layer that undergoes relatively greater reorganization in pregnancy than one or two flanking longitudinal layers. [10][11][12] There is a paucity of information about the human cervix because investigation is compromised by the impracticalities of invasive study. 13 Like other mammalian models, studies suggest a wide circumferential layer, but disagree about one versus two flanking longitudinal layers.…”

Section: Introductionmentioning

confidence: 99%

Nonlinear optical microscopy and ultrasound imaging of human cervical structure

et al. 2013

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Abstract. The cervix softens and shortens as its collagen microstructure rearranges in preparation for birth, but premature change may lead to premature birth. The global preterm birth rate has not decreased despite decades of research, likely because cervical microstructure is poorly understood. Our group has developed a multilevel approach to evaluating the human cervix. We are developing quantitative ultrasound (QUS) techniques for noninvasive interrogation of cervical microstructure and corroborating those results with high-resolution images of microstructure from second harmonic generation imaging (SHG) microscopy. We obtain ultrasound measurements from hysterectomy specimens, prepare the tissue for SHG, and stitch together several hundred images to create a comprehensive view of large areas of cervix. The images are analyzed for collagen orientation and alignment with curvelet transform, and registered with QUS data, facilitating multiscale analysis in which the micron-scale SHG images and millimeter-scale ultrasound data interpretation inform each other. This novel combination of modalities allows comprehensive characterization of cervical microstructure in high resolution. Through a detailed comparative study, we demonstrate that SHG imaging both corroborates the quantitative ultrasound measurements and provides further insight. Ultimately, a comprehensive understanding of specific microstructural cervical change in pregnancy should lead to novel approaches to the prevention of preterm birth.

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“…Cervical ripening is characterized by a high collagen solubility and collagenolytic activity, a decrease in total collagen content and an influx of inflammatory cells with increasing levels of cytokines and prostaglandins 3 . During dilatation, digestion of denatured collagen leads to further loss of collagen and, consequently, of firmness 4 . Local or ascending intrauterine infections are thought to lead to activation of all the components of the preterm labor syndrome, whereby functional loss of cervical integrity is the common terminal pathway 5 .…”

Section: Introductionmentioning

confidence: 99%

Comparison of transvaginal sonography in recumbent and standing maternal positions to predict spontaneous preterm birth in singleton and twin pregnancies

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et al. 2006

Ultrasound in Obstet & Gyne

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Changes in Water Content, Collagen Degradation, Collagen Content, and Concentration in Repeated Biopsies of the Cervix of Pregnant Cows

Cited by 39 publications

References 30 publications

Cervical diameter in relation to uterine and cervical EMG activity in early postpartum dairy cows with retained placentas after PGF2alpha induced calving

Cervical diameter in relation to uterine and cervical EMG activity in early postpartum dairy cows with retained placentas after PGF2alpha induced calving

Nonlinear optical microscopy and ultrasound imaging of human cervical structure

Comparison of transvaginal sonography in recumbent and standing maternal positions to predict spontaneous preterm birth in singleton and twin pregnancies

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