Changes in Vaginal Microbiome in Pregnant and Nonpregnant Women with Bacterial Vaginosis: Toward Microbiome Diagnostics?

Mohamed, Ibrahim; Zakeer, Samira; Azab, Marwa; Hanora, Amro

doi:10.1089/omi.2020.0096

Cited by 9 publications

(8 citation statements)

References 51 publications

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“…In consistence with others [ 8 , 15 , 38 ], our correlation analysis demonstrated that the phylum Firmicutes , the genus Lactobacillus , and the species L. iners were negatively correlated with almost all other vaginal taxa. According to Ng et al, 2021 [ 8 ], this emphasizes their well-documented exclusionary behavior in the vaginal ecosystem.…”

Section: Discussionsupporting

confidence: 80%

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Compositional Changes in the Vaginal Bacterial Microbiome of Healthy Pregnant Women across the Three Gestational Trimesters in Ismailia, Egypt

et al. 2023

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The composition of the vaginal microbiome may lead to adverse pregnancy outcomes. Normal pregnancy is associated with changes in the vaginal bacterial community composition, which tend to be more enriched with one or two Lactobacillus species promoting a healthy vagina and favorable birth outcomes. The aim of the current study was to determine compositional changes in the healthy vaginal microbiome composition during the three trimesters of pregnancy in Ismailia, Egypt using Illumina MiSeq sequencing of the V3–V4 region of the 16S rRNA. The phylum Firmicutes and the genus Lactobacillus dominated across the three trimesters of pregnancy. L. iners was the most abundant species. However, L. coleohominis and L. reuteri represented the least dominant vaginal lactobacilli. Core microbiome analyses showed the Lactobacillus genus and L. iners species to have the highest prevalence in all the samples of our study groups. The phylum Firmicutes was found to be negatively correlated with almost all other vaginal phyla during pregnancy. Likewise, a negative correlation between Lactobacillus and almost all other genera was detected, including significant negative correlations with Dialister and Prevotella. Furthermore, negative correlations of L. iners were detected with almost all other species, including a significant negative correlation with L. helveticus, G. vaginalis, S. anginosus, and S. agalactiae.

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Section: Discussionsupporting

confidence: 80%

“…No. 12888-50) [ 38 , 39 ], according to manufacturer’s instructions. Extraction steps were conducted after gentle vortex of the collection tubes containing swab tips to induce better dispersion of the trapped bacteria.…”

Section: Methodsmentioning

confidence: 99%

Compositional Changes in the Vaginal Bacterial Microbiome of Healthy Pregnant Women across the Three Gestational Trimesters in Ismailia, Egypt

et al. 2023

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“…Vaginal microbial composition is a potential future target for clinical diagnostics [10,20,24,[28][29][30]. However, the development of any diagnostic test in clinical microbiology requires straightforward and trustworthy sample-collection methods.…”

Section: Discussionmentioning

confidence: 99%

Optimal sampling and analysis methods for clinical diagnostics of vaginal microbiome

Kero

Hieta

Kallonen

et al. 2023

Eur J Clin Microbiol Infect Dis

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Next-generation sequencing-based microbiological analysis is a complex way to profile vaginal microbiome samples since each step affects the results gained. Methodologies for sample collection lack golden standards. We compared Puritan DNA/RNA swab (PS) and Copan FLOQ swab (CS) and provided consistent and reliable microbiome profiles analyzed by 16S rRNA gene sequencing. We collected two consecutive vaginal samples utilizing PS with room temperature storing and CS with instant freezing from 26 women. Variable region 4 of bacterial 16S rRNA gene was amplified with single PCR by custom-designed dual-indexed primers and sequenced with Illumina MiSeq system. Read quality control, operational taxonomic unit tables, and alpha and beta diversities analysis were performed, and community richness, diversity, and evenness were evaluated and compared between the two samplings and tests. Nineteen sample pairs produced detectable, intact DNA during the extraction protocol and/or further microbial profiles. Alpha bacterial diversity indices were independent on the collection protocol. No significant statistical differences were found in the measured beta diversity metrics between the collection methods. Of the women, 43% had Lactobacillus-dominated vaginal microbiome profile despite of collection method. Previously reported important vaginal microbiome phyla Actinobacteria, Bacteroidetes, Firmicutes, Fusobacteria, and Proteobacteria were present in the sample set although their relative abundances varied among individuals. PS and CS enable constant vaginal microbiota sampling. The PS method with no need for instant freezing is suitable for on-site collections at clinics. Furthermore, it seems to be possible to take two samples instead of one with constant microbiological results.

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“…Bacterial vaginosis (BV) is a polymicrobial disorder of the lower genital tract characterised by an alteration in the vaginal microenvironment (dysbiosis) resulting in the loss of Lactobacillus species dominance, increase in vaginal pH and a dramatic overgrowth of pathogenic Gram negative and positive facultative and obligate anaerobic bacteria such as Gardnerella, Atopobium, Bacteroides, Mobiluncus, Prevotella, Mycoplasma, Peptostreptococcus, Anaerococcus, Sneathia, Clostridium, Leptotrichia species, BV-associated bacterium 1 (BVAB1) to BVAB3 etcetera [1][2][3][4][5][6][7]. The specific vaginal bacterial composition of BV can differ between individual women [5,8,9]. However, one frequent culprit in the pathogenesis and diagnosis of BV is Gardnerella vaginalis, a non-motile, catalase-negative, Gram variable facultative anaerobic coccobacilli [6,7].…”

Section: Introductionmentioning

confidence: 99%

Mechanistic Insights into Immune Suppression and Evasion in Bacterial Vaginosis

Amabebe

Anumba

2022

Curr Microbiol

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The immunological response to bacterial vaginosis (BV) remains poorly understood and recurrent BV is still a major public health burden especially in the pregnant population. This article reviews the potential mechanisms by which BV-associated bacteria suppress and circumvent the host and microbial defence responses, and propagate their survival/dominance without overt inflammation. We discuss the composition of cervicovaginal mucosal barrier and the mechanism by which BV circumvents host defence: the degradation of the mucosal barrier and immunoglobulin A (IgA); the BV-associated organism Gardnerella vaginalis haemolysin (vaginolysin); diminished IgA response against vaginolysin; mucosal sialic acid degradation, foraging and depletion; inhibition of IL-8-induced neutrophilic infiltration; and metabolite-induced incapacitation of neutrophil and monocyte chemotaxis. We also highlight the tolerance/resistance to both host and antimicrobial molecules mounted by BV-associated biofilms. A plausible role of sialic acid-binding immunoglobulin-like lectins (SIGLECS) was also suggested. Sialidase, which is often produced by G. vaginalis, is central to the immunosuppression, relapse and recurrence observed in BV, although it is supported by other hydrolytic enzymes, vaginolysin and immunomodulatory metabolites.

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Changes in Vaginal Microbiome in Pregnant and Nonpregnant Women with Bacterial Vaginosis: Toward Microbiome Diagnostics?

Cited by 9 publications

References 51 publications

Compositional Changes in the Vaginal Bacterial Microbiome of Healthy Pregnant Women across the Three Gestational Trimesters in Ismailia, Egypt

Compositional Changes in the Vaginal Bacterial Microbiome of Healthy Pregnant Women across the Three Gestational Trimesters in Ismailia, Egypt

Optimal sampling and analysis methods for clinical diagnostics of vaginal microbiome

Mechanistic Insights into Immune Suppression and Evasion in Bacterial Vaginosis

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