Changes in transcript and protein expression levels in the barley — <i>Cochliobolus sativus</i> interaction

Jawhar, M.; Shoaib, A.; Arabi, M. I. E.; Al-Daoude, A.

doi:10.1556/0806.44.2016.047

Cited by 7 publications

(3 citation statements)

References 26 publications

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“…ex Dastur] are two globally distributed fungal pathogens of barley causing substantial yield losses (Kumar et al 2002;Rsaliyev et al 2017). Barley plants respond to both pathogens by activating different mechanisms that are regulated through different plant signaling pathways, including plant hormones such as SA and pathogenesisrelated (PR) proteins (Bindschedler et al 1998;Jawhar et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Cooperative functioning of salicylic acid and phenylalanine ammonia lyase in barley plant challenged with spot blotch and powdery mildew diseases

Al-Daoude¹,

Al-Shehadah²,

Shoiab³

et al. 2019

Acta Biol Szeged

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Salicylic acid (SA) and phenylalanine ammonia-lyase (PAL) have been suggested as important signals during plant resistance towards several fungal pathogens. In this work, to better understand the defense responses initiated by resistant and susceptible barley genotypes challenged with a necrotrophic (Cochliobolus sativus; Cs) and a biotrophic (Blumeria graminis; Bg) pathogens, the relative contributions of SA and PAL were investigated at early time points of infection. SA signaling was activated in both genotypes 24 hours post infection (hpi) as compared with the non-inoculated plants. However, with or without pathogen pretreatment, SA significantly increased (P = 0.001) in the resistant genotype that contained three-folds of total SA in comparison with the susceptible one for Bg. Reverse transcription-polymerase chain reaction (RTPCR) analysis revealed that PAL expression increases in the resistant and susceptible genotypes over the inoculation time points, with the maximum expression observed 48 hpi. PAL expression was paralleled by an increase in SA content in leaves as shown by the test coincidence (F3, 32 = 1.09, P = 0.49 for Cs and F3, 32 = 1.03, P = 0.48 for Bg). Results showed that the cooperatively function of SA and PAL in barley responses to both Cs and Bg appeared to be dependent on the plant genotype, and that SA signaling and PAL play a role in barley interactions with these both pathogens. This study might increase our understanding for a deeper molecular research on barley defense responses against pathogens with different lifestyles.

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Section: Introductionmentioning

confidence: 99%

Cooperative functioning of salicylic acid and phenylalanine ammonia lyase in barley plant challenged with spot blotch and powdery mildew diseases

Al-Daoude¹,

Al-Shehadah²,

Shoiab³

et al. 2019

Acta Biol Szeged

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“…], the cause of spot blotch (SB), is an economically important disease of barley (Hordeum vulgare) worldwide (Mathre 1997). C. sativus infection on barley plants results in various host-specific biochemical responses which show strategies deployed by the plant to withstand diseases (Kumar et al 2002;Jawhar et al 2017). These responses are regulated by a concerted expression of different plant signaling pathways, and associated with an oxidative response detectable as the localized accumulation of reactive oxygen species (ROS) molecules in attacked and/or neighboring cells of the infection site (Leng and Zhong 2015;Rodríguez-Decuadro et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Salicylic acid and hydrogen peroxide accumulation in relation to hydrolyte leakage in barley plants challenged with Cochliobolus sativus

Al-Daoude

Al-Shehadah

Shoaib

et al. 2018

Cereal Research Communications

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Spot blotch (SB) caused by the hemibiotrophic fungal pathogen Cochliobolus sativus is a destructive disease of barley worldwide. To better understand the mechanisms of resistance to this disease, the involvements of salicylic acid (SA), hydrogen peroxide (H 2 O 2) and ion fluxes during the interaction between resistant and susceptible barley seedlings and C. sativus were investigated. Early SA accumulation in leaf tissues was accompanied with an increase in H 2 O 2 concentration in both compatible and incompatible interactions. The resistant cultivar constitutively contained higher levels of H 2 O 2 and SA, as well as during the 72 h as compared with the un-infected control (0 h). However, levels increased rapidly upon infection in both cultivars. Moreover, a markedly greater increase in ion fluxes from the compatible material compared with the incompatible one was observed. Results suggest that SA and H 2 O 2 accumulation are important during both compatible and incompatible barley-C. sativus interactions.

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“…Moreover, plant cell reprogramming has been spotted in many pathosystems, including barley (Ghannam et al 2016;Huang et al 2016). A transcriptional profiling of barley genes during Rs and Cs infection revealed a complex cell reprogramming due to specific transcriptional and metabolic alterations generated by the pathogen (Jawhar et al, 2017;Al-Daoude et al 2016). However, these studies suggested that transcripts for basal plant defense genes continued to increase in plenty during the infection process.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome profiling reveals distinct gene activations in barley responding to scald and spot blotch

Shoaib

Al-Daoude

Arabi

et al. 2018

Cereal Research Communications

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Scald (Rhynchosporium secalis; Rs) and spot blotch (Cochliobolus sativus; Cs) are important diseases of barley (Hordeum vulgare L.) worldwide. Similar mechanisms and gene transcripts are assumed to be involved in the barley defense response since both these pathogens are necrotrophic fungi. In the current study, the transcriptome in leaves of the same barley genotype WI2291 inoculated with Rs and Cs was compared at different times postinoculation. Comparison of data for barley Rs-and Cs-inoculated plants with mockinoculated plants revealed gene expression changes that included basal defense transcripts and transcripts specific to the establishment of a necrotrophic interaction with associated fungi. During barley-pathogen interaction pathway, WI2291 activated a higher number of genes and pathways in response to Rs infection than in response to Cs invasion. However, families of genes encoding pectin-degrading enzymes, secondary metabolism enzymes, transporters and peptidases are expanded to cover Rs and Cs at an early stage following inoculation. Our results demonstrate differences in the pathways and activated genes of barely cv. WI291challenged by Rs and Cs, and that expression patterns of the same defenseassociated genes were altered in adaptation to different pathogens. Our work provides new insights into the underlying mechanisms related to regulation of different pathways in response to fungal infection.

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Changes in transcript and protein expression levels in the barley — Cochliobolus sativus interaction

Cited by 7 publications

References 26 publications

Cooperative functioning of salicylic acid and phenylalanine ammonia lyase in barley plant challenged with spot blotch and powdery mildew diseases

Cooperative functioning of salicylic acid and phenylalanine ammonia lyase in barley plant challenged with spot blotch and powdery mildew diseases

Salicylic acid and hydrogen peroxide accumulation in relation to hydrolyte leakage in barley plants challenged with Cochliobolus sativus

Transcriptome profiling reveals distinct gene activations in barley responding to scald and spot blotch

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