Salicylic acid and hydrogen peroxide accumulation in relation to hydrolyte leakage in barley plants challenged with Cochliobolus sativus

Abstract

Spot blotch (SB) caused by the hemibiotrophic fungal pathogen Cochliobolus sativus is a destructive disease of barley worldwide. To better understand the mechanisms of resistance to this disease, the involvements of salicylic acid (SA), hydrogen peroxide (H 2 O 2) and ion fluxes during the interaction between resistant and susceptible barley seedlings and C. sativus were investigated. Early SA accumulation in leaf tissues was accompanied with an increase in H 2 O 2 concentration in both compatible and incompatible interactions. The resistant cultivar constitutively contained higher levels of H 2 O 2 and SA, as well as during the 72 h as compared with the un-infected control (0 h). However, levels increased rapidly upon infection in both cultivars. Moreover, a markedly greater increase in ion fluxes from the compatible material compared with the incompatible one was observed. Results suggest that SA and H 2 O 2 accumulation are important during both compatible and incompatible barley-C. sativus interactions.

A novel effector, CsSp1, from Bipolaris sorokiniana, is essential for colonization in wheat and is also involved in triggering host immunity

“…It is well known that salicylic acid (SA) is involved in the plant immune response against biotrophic and hemibiotrophic pathogens, and is associated with the induction of plant systemic acquired resistance (SAR) (Alvarez, 2000 ; Meenakshi & Singh, 2013 ; Vlot et al, 2009 ). SA accumulates during both incompatible and compatible interactions between B. sorokiniana pathogens and host plants to facilitate resistance to spot blotches (Al‐Daoude, 2019 ; Al‐Daoude et al, 2018 ; Sahu et al, 2016 ). Lesion development is associated with the accumulation of host‐encoded pathogenesis‐related (PR) proteins and reactive oxygen species (ROS) (Ajith et al, 2003 ).…”