Changes in Total Homocysteine and Glutathione Levels After Laparoscopic Sleeve Gastrectomy in Children with Metabolic-Associated Fatty Liver Disease

Pastore, Anna; Panera, Nadia; Mosca, Antonella; Caccamo, Romina; Camanni, Daniela; Crudele, Annalisa; Stefanis, Cristiano De; Alterio, Arianna; Giovamberardino, Gianna Di; Vito, Rita De; Francalanci, Paola; Battaglia, Sonia; Muda, Andrea Onetti; Peppo, Francesco De; Alisi, Anna

doi:10.1007/s11695-021-05701-6

Cited by 6 publications

(7 citation statements)

References 37 publications

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“…Several studies have highlighted the clinical impact of homocysteine on obesity [12][13][14][15]. This is corroborated in this study, as we found that Hcy is significantly higher in patients with obesity with respect to healthy children.…”

Section: Discussionsupporting

confidence: 90%

“…Accordingly, we found dramatically lower levels of GSH in our children with obesity. Our previous studies report an excess of GSH utilization in obesity [14,15], and Nguyen and collaborators [28] suggest that GSH deficiency can promote fat accumulation. They found that mice with GSH deficiency had significantly higher total body fat, and that restoring GSH led to significant fat reductions, suggesting a role for GSH in maintaining fat homeostasis.…”

Section: Discussionmentioning

confidence: 95%

“…As several studies have highlighted the clinical impact of thiol-disulfide hemostasis on obesity [10][11][12][13][14][15], we analyze thiol levels in our cohort of children with obesity. Moreover, we investigate serum markers of oxidative stress in order to verify if ROS overproduction could contribute to the development of complications in obesity.…”

Section: Introductionmentioning

confidence: 99%

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Study of the Association between Thiols and Oxidative Stress Markers in Children with Obesity

et al. 2022

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Obesity has reached epidemic proportions, and the World Health Organization defined childhood overweight and obesity as a noncommunicable disease that represents the most serious public health challenges of the twenty-first century. Oxidative stress, defined as an imbalance between oxidants and antioxidants causing an impairment of the redox signals, is linked to the development of metabolic diseases. In addition, reactive oxygen species generated during metabolic disorder could increase inflammation, causing the development of insulin resistance, diabetes, and cardiovascular disease. We analyze serum levels of cysteine (Cys), cysteinyl-glycine (Cys-Gly), homocysteine (Hcy), and glutathione (GSH), and other markers of oxidative stress, such as thiobarbituric acid reactive substances (T-BARS), 8-isoprostane, and protein carbonyl in our children with obesity. Total antioxidant status was also determined. We found lower GSH and Cys-Gly levels, and higher Hcy and oxidative stress markers levels. We also found a positive correlation between Body Mass Index (BMI), Cys, GSH, and Hcy levels, between insulin and Cys levels, and between BMI and the homeostasis model assessment-estimated insulin resistance (HOMA-IR) with 8-isoprostane levels. Finally, we found a correlation between age and GSH and Cys levels. The deficiency of GSH could be restored by dietary supplementation with GSH precursors, supplying an inexpensive approach to oppose oxidative stress, thus avoiding obesity complications.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

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Study of the Association between Thiols and Oxidative Stress Markers in Children with Obesity

et al. 2022

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“…Thiols and vitamins D 2 and D 3 determinations. Plasma thiols were analyzed as previously reported 44 . For plasma VD 2 and VD 3 determinations, we use the MassTrak Vitamin D Solution from Waters, together with the ACQUITY UPLC I-Xevo TQ-S micro IVD (Waters Chromatography Europe BV, Etten-Leur, The Netherlands).…”

Section: Methodsmentioning

confidence: 99%

Contribution of vitamin D3 and thiols status to the outcome of COVID-19 disease in Italian pediatric and adult patients

D'Alessandro

Ciavardelli

Pastore

et al. 2023

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The coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARSCoV-2), was declared a global pandemic by the World Health Organization (WHO) on March 2020, causing unprecedented disease with million deaths across the globe, mostly adults. Indeed, children accounted for only a few percent of cases. Italy was the first Western country struck by the COVID-19 epidemic. Increasing age, which is one of the principal risk factors for COVID-19 mortality, is associated with declined glutathione (GSH) levels. Over the last decade, several studies demonstrated that both vitamin D (VD) and GSH have immunomodulatory properties. To verify the association between VD, GSH and the outcome of COVID-19 disease, we conducted a multicenter retrospective study in 35 children and 128 adult patients with COVID-19. Our study demonstrated a hypovitaminosis D in COVID-19 patients, suggesting a possible role of low VD status in increasing the risk of COVID-19 infection and subsequent hospitalization. In addition, we find a thiol disturbance with a GSH depletion associated to the disease severity. In children, who fortunately survived, both VD and GSH levels at admission were higher than in adults, suggesting that lower VD and thiols levels upon admission may be a modifiable risk factor for adverse outcomes and mortality in hospitalized patients with COVID-19.

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“…Thiols (cysteine [Cys], cysteinyl-glycine [Cys-Gly], glutathione [GSH], and homocysteine [Hcy]), the major non-enzymatic antioxidant compounds, were analyzed as previously reported (Pastore et al, 2022).…”

Section: Methodsmentioning

confidence: 99%

Influence of Defatting and Pasteurization on Nutrients and Oxidative Stress Markers in Human Milk

et al. 2023

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Background: It is well known that the best nutritional option for infants is human milk, and that when breastfeeding is not possible, human milk banks are a possible alternative. However, in the case of infants with fat transport disorder like chylothorax, defatting of human milk is mandatory. Research Aim: The aim of the study was to reduce milk fat content without reducing other nutrients, increasing oxidative stress, or introducing harmful microorganisms. Methods: In this prospective, cross-sectional, observational study, we examined the influence of defatting and pasteurization of 50 donor samples on fat, macro- and micronutrients, as well as on oxidative stress markers. Results: Low-temperature centrifugation proved to be very efficient in defatting, reducing the concentration of triglycerides by 85% and cholesterol by 50%. The macronutrients (proteins, albumin, and Immunoglobulin A) did not undergo significant changes due to defatting and pasteurization procedures, while iron decreased by 36%. However, as the majority of iron is retained, this result does not remarkably change the milk composition. Furthermore, oxidative stress markers and antioxidant levels were unchanged, and the milk result was microbiologically safe. Conclusions: Cold milk centrifugation proved to be an effective technique that allows the reduction of human milk lipids. The determination of triglycerides and cholesterol can be used as an indicator of skimming. This procedure is not accompanied by substantial modifications of other components present in the milk.

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Changes in Total Homocysteine and Glutathione Levels After Laparoscopic Sleeve Gastrectomy in Children with Metabolic-Associated Fatty Liver Disease

Cited by 6 publications

References 37 publications

Study of the Association between Thiols and Oxidative Stress Markers in Children with Obesity

Study of the Association between Thiols and Oxidative Stress Markers in Children with Obesity

Contribution of vitamin D3 and thiols status to the outcome of COVID-19 disease in Italian pediatric and adult patients

Influence of Defatting and Pasteurization on Nutrients and Oxidative Stress Markers in Human Milk

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