Changes in the structures of motile sperm subpopulations in dog spermatozoa after both cryopreservation and centrifugation on PureSperm® gradient

Dorado, J.; Alcaráz, L.; Duarte, N.; Portero, J. M.; Acha, D.; Hidalgo, M.

doi:10.1016/j.anireprosci.2011.03.013

Cited by 39 publications

(38 citation statements)

References 63 publications

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“…Our study identified four discrete sperm subpopulations in unselected and selected samples from chilled dog semen, which agrees with previous studies in dogs (Dorado and others 2011b, Dorado and others 2011c). In our study, SLC through AND significantly modified the distribution of spermatozoa within subpopulations.…”

Section: Discussionsupporting

confidence: 92%

“…Recent studies have used multistep procedures (non-hierarchical and hierarchical methods) to study the sperm population structure in the dog (Núñez-Martínez and others 2006, Dorado and others 2011c). Besides, the presence of separate motile sperm subpopulations in the dog has been associated with the ability to withstand the adverse effects of cryopreservation (Dorado and others 2011b, Dorado and others 2013c) and storage (Dorado and others 2011c). The characterisation of sperm subpopulations in canine ejaculates has been applied in various studies to compare different cooling extenders (Dorado and others 2011c) or different sperm selection techniques (Dorado and others 2013b, Dorado and others 2013c).…”

Section: Introductionmentioning

confidence: 99%

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Should single layer centrifugation of dog semen be done before or after the semen is cooled?

et al. 2015

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The aim of this study was to assess the effectiveness of sperm selection by single layer centrifugation (SLC) on canine sperm quality when SLC was performed before or after the cooling process, or when double SLC (before and after cooling) was performed. Twenty ejaculates from four dogs were divided into four aliquots as follows: unselected: no SLC was performed; SLC prior to cooling (SLC-PC): sperm selection was carried out before cooling; SLC after cooling (SLC-AC): sperm selection was performed after cooling; and double SLC: sperm selection was carried out before and after cooling. Sperm motility (by computer-assisted semen analysis), morphology (Diff-Quick staining), sperm membrane integrity (Vital-Test kit) and acrosome integrity (double fluorescent stain) were assessed in re-warmed semen samples. Four sperm subpopulations (sP) were detected using a pattern analysis technique (sP1: highly active, non-progressive; sP2: low velocity, highly progressive; sP3: less vigorous, poorly progressive; sP4: highly progressive motility). A higher proportion of sperm were classified as sP4 in SLC-AC samples. Most of the sperm parameters assessed showed higher values in the SLC-AC group. We conclude that SLC-AC is the best protocol to improve sperm quality in chilled canine semen in comparison to the other procedures tested.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Should single layer centrifugation of dog semen be done before or after the semen is cooled?

et al. 2015

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“…Comparable results were also found in experiments with goats (Jimenez-Rabadan et al, 2012), equines (Anja et al, 2013) and dogs (Dorado et al, 2011), which employed speciespecific SLC methods.…”

Section: Discussionsupporting

confidence: 66%

“…TM and PM have a positive correlation with sperm viability (Maia et al, 2009) and can interfere in the fertility of semen because these parameters are closely related to the capacity of the sperm cell to achieve the fecundation site (Dorado et al, 2011). Significant improvements in the TM, PM and %RAP of the samples after selection in silica colloidal-silane were found.…”

Section: Discussionmentioning

confidence: 81%

Kinematic and spermatic recovery after selection by centrifugation in colloid solutions of ovine cryopreserved semen

Bergstein

Bicudo

Rodello

et al. 2016

Arq. Bras. Med. Vet. Zootec.

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Frozen and thawed ovine semen undergo morphological and functional changes that prevent or decrease the efficiency of fertilization. Sperm selection methods seek to improve the quality and viability of the fertilizing materials. Four sperm selection methods were employed, using two silica colloidal solutions coated with silane (silica colloidal-silane) or by polyvinylpyrrolidone (silica colloidal-PVP), and varying the volume of colloidal solution. Sperm kinematic and sperm recovery were evaluated by means of CASA. The protocols using silica colloidal-silane showed higher total motility (TM), progressive motility (PM) and percentage of rapid sperm (%RAP) compared to the methods employing silica colloidal-PVP and to the samples prior to sperm selection. The silica colloidal-PVP had greater sperm recovery compared to the silica colloidal-silane. Only the method using 4mL of silica colloidal-PVP was not efficient in selecting samples with better quality compared to the samples analyzed prior to sperm selection. The methods using lower volumes of colloidal solution did not differ from those using higher volumes and the best results were shown by the method with 1mL silica colloidal-silane. The results found in the study indicated greater efficiency of the silica colloidal-silane solution for sperm selection of thawed ovine semen when compared to selection using silica colloidal-PVP. The method using 1mL of silica colloidal-silane was equally efficient to the method with higher volume, presenting itself as an alternative to process samples with lower sperm concentration.

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“…However, it has been pointed out that the sperm of some species is not a homogeneous mixture, and different sperm subpopulations coexist in the same sample. Although this topic has mostly been studied in mammals (Quintero-Moreno et al, 2003;Dorado et al, 2011), there are some reports in fish which have clearly shown the coexistence of distinct motility-based sperm subpopulations within the same sample : Solea senegalensis (Martinez-Pastor et al, 2008), Oncorhynchus mykiss (Kanuga et al, 2012) or Anguilla anguilla (Gallego el al. , submitted b).…”

Section: General Introduction 21mentioning

confidence: 99%

Sperm physiology and quality in two marine teleosts: Anguilla anguilla & Takifugu niphobles

Albiach¹

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ACKNOWLEDGEMENTSMuchos años han pasado desde que en aquel verano de 2007, cuando aún era un biólogo casi recién salido del horno, empecé mi carrera investigadora gracias a una beca en el grupo de investigación en el que hoy, unos años más viejuno, presento mi pequeña aportación a la ciencia. Durante todo este periodo he pasado buenos y malos momentos, mejores y peores rachas, pero sobretodo, he vivido una experiencia difícil de olvidar, o más bien inolvidable, en la que he podido madurar como persona y como investigador gracias a todas las personas que me han acompañado durante estos maravillosos años. Son muchos los agradecimientos que tengo que dar y poca la memoria, así que pido perdón por si alguien cae en el olvido, aunque no hay nada que no se pueda solucionar con una cerveza fresquita! En primer lugar, quería dar gracias a mi familia, que aunque nunca se lo diga y muchísimas veces parezca un "desapegao"… les quiero un montón. Ya hacen 33 primaveras que "el pare" y "la máaaama" (si, con acento) me trajeron al mundo, porque ya sabemos que lo que va primero es el oocito y el espermatozoide (Chapter 5… jjjjj), así que mil gracias por el cariño y el apoyo incondicional que me habéis dado durante todos estos años. En el mismo pack entra mi querida hermana "mongola" (uooooooooo… esto queda publicado en libro teta!!!), que también ha estado ahí y siempre me ha echado un cable cuando ha podido, aunque sea para decir… "tete no llegues tarde que mañana tienes laboratorio!!!" (si el microscopio o el citómetro hablaran...).A Paty (mi monito), por tu amor, comprensión y apoyo infinito durante todos estos años. Aún recuerdo el primer día que te conocí andando por el departamento y tu sonrisa se quedó grabada en mi memoria (que por cierto te invité a comer y me dijiste que… noooooooo!! toma xafun!!). Supongo que nos empezamos a querer en Monfragüe viendo y descubriendo pajarines mientras oíamos como cantaba el búhito, así que mil gracias por estar conmigo en los buenos y malos momentos, por ser un apoyo incondicional para mí, por quererme, y por todos estos años de sonrisas que hemos pasado juntos… que han sido muchos y muy bonitos.A mis amigos de toda la vida!!! Of course!!! que han estado ahí y estarán durante muchos años. Realmente es un grupo heterogéneo, que va desde los gorilillas de mi equipo de balonmano hasta algunos con los que empecé a gatear en preescolar. Que bien me lo he pasado en los torneos balonmaneros de San Esteve, donde dimos nuestros primeros pasos internacionales en materia de balonmano y en materia de bebercios!!! Mención especial merece mi amigo Caniggi, que a pesar de su estresante vida (tiene una pizzería, un huerto, un trabajo, un perro… y encima novia!!!) aun saca ratillos para jugar a pádel (hombre él jugar jugar… no juega… jjjjj) y recordad viejos tiempos de la infancia cuando aún éramos unos críos.Mucha gente es con la que he compartido laboratorio y a la que quería dar las gracias de manera especial. Realmente no estaría bien hablar de compañeros de laboratorio, porque ahora muchos de ell...

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Changes in the structures of motile sperm subpopulations in dog spermatozoa after both cryopreservation and centrifugation on PureSperm® gradient

Cited by 39 publications

References 63 publications

Should single layer centrifugation of dog semen be done before or after the semen is cooled?

Should single layer centrifugation of dog semen be done before or after the semen is cooled?

Kinematic and spermatic recovery after selection by centrifugation in colloid solutions of ovine cryopreserved semen

Sperm physiology and quality in two marine teleosts: Anguilla anguilla & Takifugu niphobles

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