2016
DOI: 10.1590/1678-4162-8912
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Kinematic and spermatic recovery after selection by centrifugation in colloid solutions of ovine cryopreserved semen

Abstract: Frozen and thawed ovine semen undergo morphological and functional changes that prevent or decrease the efficiency of fertilization. Sperm selection methods seek to improve the quality and viability of the fertilizing materials. Four sperm selection methods were employed, using two silica colloidal solutions coated with silane (silica colloidal-silane) or by polyvinylpyrrolidone (silica colloidal-PVP), and varying the volume of colloidal solution. Sperm kinematic and sperm recovery were evaluated by means of C… Show more

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Cited by 4 publications
(4 citation statements)
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“…The method that used 1 ml colloidal silica–silane was the most effective in selecting semen with desirable characteristics, and reduced the incidence of spermatozoa with undesirable characteristics, such as damaged plasma membranes or reacted acrosomes. The method that used 1 ml colloidal silica–silane was the best method identified in this study corroborating the results reported by (Bergstein, Bicudo, Rodello, Weiss, & Bicudo, ) who studied the kinematics of ovine cryopreserved semen selected by colloidal silica–silane.…”
Section: Discussionsupporting
confidence: 90%
“…The method that used 1 ml colloidal silica–silane was the most effective in selecting semen with desirable characteristics, and reduced the incidence of spermatozoa with undesirable characteristics, such as damaged plasma membranes or reacted acrosomes. The method that used 1 ml colloidal silica–silane was the best method identified in this study corroborating the results reported by (Bergstein, Bicudo, Rodello, Weiss, & Bicudo, ) who studied the kinematics of ovine cryopreserved semen selected by colloidal silica–silane.…”
Section: Discussionsupporting
confidence: 90%
“…The results obtained after centrifugation and different fractions isolation suggest that SLC does not separate the best motility quality subpopulation, whether in C800 or in C300 sperm samples, not being SP2 the most abundant subpopulation in F3, as expected. It was observed that many spermatozoa belonging to SP2 (considered as the best subpopulation) were retained in the second interface; one possible explanation for this observation is that the high number of spermatozoa included in the sample may impede other optimal spermatozoa from swimming to the bottom of the tubes (30) . As previously recommended (31) , SLC it is better to use for processing of large volumes of semen, suggesting that the increment of the sperm concentration was an impediment to the efficient operation of this method.…”
Section: Discussionmentioning
confidence: 99%
“…Ovine ejaculate is composed of a heterogeneous population of sperm cells (Bergstein et al, 2016;García-Álvarez et al, 2010). The…”
Section: Discussionmentioning
confidence: 99%