Changes in the spectrum of proteins and phospholipids in tissues of rats exposed to thiosulfonates

Abstract

Esters of thiosulfoacids demonstrate a wide range of biological activity. One of their effects is the influence on the metabolism of proteins and lipids in the body. Therefore, the purpose of our experiment was to study the impact of synthesized thiosulfonates on the total content of proteins and phospholipids, as well as their spectrum in the blood, liver, and kidney of rats. For the experiment, allyl, ethyl, and methyl esters of thiosulfoacid were used. The protein profile of rat tissues was investigated by electrophoresis, and the ratio of different fractions of phospholipids by thin-layer chromatography. Our results have shown that short-term administration of thiosulfonates in a dose of 300 mg/kg of body weight did not cause significant changes in the content of total protein and its fractions in liver tissue, whereas the effect of allyl and ethyl esters of thiosulfoacid was accompanied by an increase in the total protein and albumin in the blood plasma. The decrease in total protein was found in the kidney tissue of rats injected with allyl- and methyl thiosulfonates. The newly synthesized compounds did not lead to significant changes in the total content of phospholipids in blood plasma and tissues of rats, except for methyl thiosulfonate, the effect of which was accompanied by an increase in the total phospholipids in the liver of rats. These data may indicate an adaptive reaction of the rat’s organism. Tissue-specific features of the phospholipid spectrum were detected in rats after short-term exposure to thiosulfonates. The most significant effect on the phospholipid profile in the blood was shown for allyl- and ethyl esters of thiosulfoacid. Their action was accompanied by a decrease in the phosphatidylserine and phosphatidylinositol fractions, while phosphatidylethanolamine and phosphatidylcholine increased, respectively. Esters of thiosulfoacid significantly influenced the ratio of different fractions of phospholipids in the liver and kidney tissues. The phospholipid composition of the liver was more influenced by the allyl and methyl esters of thiosulfoacid, whereas for the kidney tissue a greater effect was observed for ethyl and methyl esters. Thus, the action of allyl ester of thiosulfoacid caused a decrease in the asymmetry coefficient of hepatocyte membranes, indicating an elevation of the lipid bilayer saturation and the increase of membrane microviscosity. Similar changes were found in the kidney of rats treated with allyl- and ethyl thiosulfonates.

Effects of ethylthiosulfanylate and chromium (VI) on the state of pro/antioxidant system in rat liver

“…Previous studies have shown that ethylthiosulfanylate is involved in regulation of the activity of the lipid metabolism enzymes and performs the redistribution of lipid classes and a decrease in mono-, di-, triglycerides and free fatty acids in the rat liver [8]. Administration of ethylthio sulfanylate induces the increase of total protein and albumin content in rat blood plasma [12]. Also, ethylthiosulfanylate is an effective agent against fungal infections and has antimicrobial effect [9].…”

New Technologies of Effective Protection of Textiles Against Microbiological Damage

“…Тіосульфонатні біоциди ще не були апробовані в легкій промисловості, і нами вперше було вирішено експериментально дослідити антимікробні властивості даних препаратів для текстилю. Це продиктовано не тільки широким спектром дії тіосульфонатних сполук, але й спробою вирішити проблему пошуку малотоксичних та екологічних біоцидів [25,26]. Дані препарати, на нашу думку, можуть ідеально підходити під термінологію «екологічні біоциди», оскільки вони є також діючою субстанцією для лікування різних мікозів шкіри й оніхомікозу нігтів, конкурентоздатного з нізоралом і клотрімазолом.…”

S-Methyl-(2-Methoxycarbonylamino-Benzimidazole-5) Thiosulfonate as a Potential Antiparasitic Agent—Its Action on the Development of Ascaris suum Eggs In Vitro

“…Therefore, ETS affects the metabolism of nucleic acids, which contain amino groups, as well as proteins and amino acids with disulfide and sulfide groups [22]. Thiosulfonates also have a positive influence on metabolic processes in different tissues of rat organisms [23,24]. Furthermore, a thiosulfonate derivative of benzimidazole was tested for its effectiveness as an anticancer agent and showed significant growth inhibition against human tumor cell lines derived from several neoplastic diseases, e.g., leukemia, breast or prostate cancer [25].…”