S-Methyl-(2-Methoxycarbonylamino-Benzimidazole-5) Thiosulfonate as a Potential Antiparasitic Agent—Its Action on the Development of Ascaris suum Eggs In Vitro

Abstract

Ascaris suum is a soil-transmitted parasite causing ascariasis in pigs, largely limiting livestock production globally. Searching for new drugs affecting all stages of nematode development is necessary and widely postulated. The in vitro activity of S-methyl-(2-methoxycarbonylamino-benzoimidasole-5) thiosulfonate on A. suum developing eggs was studied. Five concentrations of the drug were used—0.625, 1.25, 2.5, 5 and 10 mM during 24, 48 and 72 h of exposure. After drug treatment, the eggs were washed and cultured in 0.05 M HCl at 27 °C for 20 days. Both the concentration and duration of the drug exposure had an inhibitory impact on the percentage of L2 larvae developed. The best effect was obtained after 72 h of incubation in 5 mM drug solution, only 1.9 ± 3.3% of the larvae developed to the L2 stage. Moreover, no SNP was detected at codon 167, which is correlated with benzimidazole resistance, in the tested samples. For the first time, it has been demonstrated that S-M-(2-MKA-BZ-5)TS seems to be a potential ovicidal anti-helminthic agent. It may lead to the elimination of parasites and reduce environmental contamination from roundworm eggs. The ovicidal effects of the drug should be additionally confirmed by further infection studies using experimental animals.

Prooxidant-antioxidant profile in tissues of rats under the action of thiosulfonate esters

“…Under normal physiological conditions, LPO is at a low level. It is maintained thanks to the balance of pro-and antioxidants, which, in turn, are important components of the body's homeostasis [23]. LPO is normally a vital link in the regulation of many membrane-dependent processes: the regulation of the permeability and transport of substances through the membrane, the regulation of membrane-associated enzymes and the lipid composition of membranes, the synthesis of prostaglandins, leukotrienes, thromboxanes, steroid hormones, cholesterol, and catecholamine metabolism [37].…”

“…They are of particular interest as biologically active substances in pharmaceutical and agrochemistry, as well as reagents in organic synthesis [17]. It is known that synthetic esters of thiosulfonic acids exhibit a wide range of biological activity [18][19][20][21][22][23]. In particular, their antimicrobial activity often exceeds the effectiveness of their natural counterparts [18].…”

“…However, esters of thiosulfonic acids, exhibiting antimicrobial [19], antiparasitic [23] and antitumor [22] effects, can also affect normal cells. When using antioxidants in feed, it is necessary to know whether they react with free radicals and neutralize them and what effect they have on the antioxidant system in various animal organs.…”

The Effects of Allium sativum L., Artemisia absinthium L., Cucurbita pepo L., Coriandrum sativum L., Satureja hortensis L. and Calendula officinalis L. on the Embryogenesis of Ascaris suum Eggs during an In Vitro Experimental Study

“…Finally, a suspension of non-embryonated eggs (12,000 eggs/mL) was obtained. The exposure of eggs to alcoholic plant extracts occurred in the same day [54][55][56].…”

Biocidal preparations based on thiosulphonates and biosurfactants

“…Thiosulfonates exhibit a wide spectrum of antimicrobial activity and are more stable than allicin. These compounds are already promising as preservatives, antimicrobial, insecticidal [3,4], antitumor [5], antithrombotic [6] substances.…”