Changes in the Solid-, Liquid-, and Epithelium-Associated Bacterial Communities in the Rumen of Hu Lambs in Response to Dietary Urea Supplementation

Li, Zhipeng; Mu, Chunlong; Xu, Yixuan; Shen, Junshi; Zhu, Weiyun

doi:10.3389/fmicb.2020.00244

Cited by 32 publications

(40 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In line with previous studies [15,18,26], the RE bacterial diversity was lower than that in the RS or the RL fraction (Table 2), and the bacterial composition was greatly distinct from that of the RS or the RL (Table 3). At the phylum level, contrary to the finding in the RS or the RL, Firmicutes was the first while Bacteroidetes the second largest phyla in the RE microbiota.…”

Section: Differences In Microbiota Between Epithelial and The Solid Osupporting

confidence: 91%

“…Consistent with previous studies using PCR-DGGE [17] or high-throughput sequencing [15,18,26], RS and RL shared more bacteria when compared to RE. In addition, the predicted functions and the qPCR results of the microbial groups further demonstrated that there were great differences in the structure and function of the microbiota occupying different REN.…”

Section: Effects Of Ruminal Ecological Niche On Microbiota Structure supporting

confidence: 91%

“…The bacterial richness and taxonomic composition in the RS and RL were similar, suggesting continuous exchange between these two fractions [15]. However, some taxa had different relative abundance in these two niches, a finding consistent with other reports [15,18,26] and likely reflecting specialized niches related to digestion of soluble components vs. dietary fiber [23].…”

Section: Differences In Microbiota Between Rumen Solid and Liquid Frasupporting

confidence: 89%

“…Corroborating the finding of previous studies [26,48,49], Desulfobulbus, one genus of SRB [50], was the predominant SRB genus (5.22%) in RE microbiota (Table 3). In line with the high-throughput sequencing result, the qPCR results also demonstrated that SRB were more predominant on RE than in the RS or the RL fraction.…”

Section: Differences In Microbiota Between Epithelial and The Solid Osupporting

confidence: 83%

“…To our knowledge, most of these studies using dairy cattle or steers as experimental animals, and only one study, which used PCR-DGGR [17], investigated the bacterial community occupying the different REN of growing lambs. Recently, we also evaluated the changes of the bacterial communities at the three different REN of growing Hu lambs in response to dietary urea supplementation [26]. However, most of the studies focused only on the community structure and function of rumen bacteria, without considering the eukaryotes [27].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Effects of dietary replacement of soybean meal with dried distillers grains with solubles on the microbiota occupying different ecological niches in the rumen of growing Hu lambs

Shen

et al. 2020

J Animal Sci Biotechnol

Self Cite

View full text Add to dashboard Cite

Background: Diet has a profound impact on the rumen microbiota, and the impact can vary among the different rumen ecological niches (REN). This study investigated the effects of dietary replacement of soybean meal (SBM) with dried distillers grains with solubles (DDGS) on the rumen microbiota occupying different REN of growing Hu lambs. After a 9-week feeding trial, 6 lambs from each dietary treatment (SBM vs. DDGS-based diets) were slaughtered for sample collection. The microbiota of the rumen solid, liquid, and epithelium fractions was examined using amplicon sequencing analysis of bacterial 16S rRNA gene, functional prediction, and qPCR. Results: No interaction of dietary protein source (PS) and REN were detected for virtually all the measurements made in this study. The DDGS substitution resulted in very limited influence on bacterial community structure. However, the metabolic pathways predicted from 16S rRNA gene sequences varied greatly between SBM-and DDGS-based diets. The populations of rumen total bacteria, fungi, sulfate-reducing bacteria (SRB), and methanogens were not influenced by DDGS substitution, but the population of protozoa was reduced. The bacterial communities in rumen solid (RS) and liquid (RL) were similar in taxonomic composition but were different in relative abundance of some taxa. In contrast, the bacterial composition and relative abundance of rumen epithelium (RE) were greatly distinct from those of the RS and the RL. In alignment with the bacterial relative abundance, the metabolic pathways predicted from 16S rRNA genes also varied greatly among the different REN. The populations of total bacteria, protozoa, and methanogens attached to the RE were smaller than those in the RS and RL, and the fungal population on the rumen epithelium was smaller than that in the RS but similar to that in the RL. On the contrary, the SRB population on the RE was greater than that in the RS and RL.

show abstract

Section: Differences In Microbiota Between Epithelial and The Solid Osupporting

confidence: 91%

Section: Effects Of Ruminal Ecological Niche On Microbiota Structure supporting

confidence: 91%

Section: Differences In Microbiota Between Rumen Solid and Liquid Frasupporting

confidence: 89%

Section: Differences In Microbiota Between Epithelial and The Solid Osupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effects of dietary replacement of soybean meal with dried distillers grains with solubles on the microbiota occupying different ecological niches in the rumen of growing Hu lambs

Shen

et al. 2020

J Animal Sci Biotechnol

Self Cite

View full text Add to dashboard Cite

show abstract

In vitro protein protection of protein meals using Bioprotect and tannin extract from red grape marc

Tunkala,

DiGiacomo,

Alvarez Hess

et al. 2024

Animal Research and One Health

View full text Add to dashboard Cite

Protecting crude protein in the rumen may reduce extensive protein degradation and ammonia emission and increase available bypass protein in ruminants. This experiment was conducted to determine the effect of two Bioprotect (15 and 30 mL/kg dry matter (DM)) and two tannin extract (TE) (20 and 40 g/kg DM) inclusion rates on protein protection and in vitro fermentation characteristics of canola and soybean meals incubated for 24 h using an ANKOM in vitro gas production system. The treated canola and soybean meals produced lower soluble protein (fraction ‘a’) and larger slowly degradable protein (fraction ‘b’) than its untreated counterparts, p < 0.01. However, the 20 g/kg DM TE inclusion showed lowest effect on the amount of protein fractions ‘a’ and ‘b’ in both meals compared to their other treated counterparts. The increasing concentration of additives reduced the total volatile fatty acids (VFA), p < 0.001. The effects of additives differed between the treatments as 15 mL/kg DM Bioprotect and 20 g/kg DM TE did not affect the acetic to propionic acid ratio (A:P) and the time before gas production began. The increase in fraction ‘b’ and reduction in protein fraction ‘a’ confirm successful protein protection in this experiment. However, the extensive reduction in ammonia‐N and in vitro degradable protein after using 30 mL/kg DM Bioprotect suggests possible toxicity to the microbes responsible for protein digestion in higher doses. Therefore, 15 mL/kg DM Bioprotect and 40 g/kg DM TE could be promising protein protection doses for in vitro experiments.

show abstract

Exploring the rumen microbiota of Hu lambs in response to diet with paper mulberry

Wang

Guo

et al. 2023

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Changes in the Solid-, Liquid-, and Epithelium-Associated Bacterial Communities in the Rumen of Hu Lambs in Response to Dietary Urea Supplementation

Cited by 32 publications

References 72 publications

Effects of dietary replacement of soybean meal with dried distillers grains with solubles on the microbiota occupying different ecological niches in the rumen of growing Hu lambs

Effects of dietary replacement of soybean meal with dried distillers grains with solubles on the microbiota occupying different ecological niches in the rumen of growing Hu lambs

In vitro protein protection of protein meals using Bioprotect and tannin extract from red grape marc

Exploring the rumen microbiota of Hu lambs in response to diet with paper mulberry

Contact Info

Product

Resources

About