Exploring the rumen microbiota of Hu lambs in response to diet with paper mulberry

Wang, Xiaotian; Li, Xiaomei; Guo, Linna; Fang, Yang; Xiong, Yi

doi:10.1007/s00253-023-12614-0

Cited by 5 publications

(3 citation statements)

References 44 publications

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“…Compared with the sheep in groups AZ and BZ (164), the shared ASVs between AZ and CZ (109), AZ (113), DZ (118), and AZ and EZ were found to be fewer (Figure 1), which indicated a difference in the microbiota of the ST sheep fed with concentrated feed. No remarkable differences in the Alpha diversity index were found among the ST sheep groups, which is in accordance with Hu lambs fed with different diets [26] and dogs fed with different diets [27]. However, these results are not in line with those reported in Tibetan sheep fed with different forage types [28].…”

Section: Discussioncontrasting

confidence: 57%

Effect of Different Feeds on the Fungi Microbiome of Suffolk Crossed with Tibetan Sheep

Ren,

Ciwang,

Wang

et al. 2023

Life

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The gut microbiome plays an important role in the metabolism, nutrient absorption and immunocompetency of animals. The dynamics of the microbiota can be influenced by modulatory factors that involve nutrition, environment, health, diseases, etc. Few reports have been documented regarding the effects of different feeds on the fungi microbiome of Suffolk crossed with Tibetan sheep. A total of 30 Suffolk crossed with Tibetan sheep (ST sheep) were selected for the study and randomly divided into five equal groups (n = 6): AZ, BZ, CZ, DZ and EZ. Group AZ was fed with alfalfa and oat grass, whereas group BZ was fed with mixture of concentrated feed, alfalfa and oat grass. Groups CZ, DZ and EZ were fed with concentrated feed #1, #2 and #3, respectively. All experimental animals were fed twice a day for four months, and rectum samples were collected for microbiota analysis. Results revealed that 2,781,461 raw reads and 2,333,239 clean reads were achieved in the ST sheep. When compared with the sheep of groups AZ and BZ (164), the shared amplicon sequence variants (ASVs) between AZ and CZ (109), AZ (113) and DZ (118) as well as AZ along with EZ were fewer. Conspicuous different phyla (8) and genera (56) were examined and compared with free-range sheep in AZ. Genera including Xeromyces, Kazachstania, Cordyceps, Rhodotorula, Pichia, Spor, etc. were found higher in animals in the CZ, DZ and EZ groups. The results of this study provide new insights regarding the effects of different feeds on the fungi microbiome of sheep farmed on the plateau. We concluded that the differences in feed in Suffolk crossed with Tibetan sheep altered their gut microbiota.

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Section: Discussioncontrasting

confidence: 57%

Effect of Different Feeds on the Fungi Microbiome of Suffolk Crossed with Tibetan Sheep

Ren,

Ciwang,

Wang

et al. 2023

Life

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“…Prevotella , the dominant bacterium in the YC group, plays a role in carbohydrate utilization, nitrogen metabolism, and fiber degradation ( Li et al, 2020 ; Xiong et al, 2023 ). It belongs to the phylum Bacteroidetes and is the core bacterium with the highest richness in the rumen ( Henderson et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Mechanistic insights into rumen function promotion through yeast culture (Saccharomyces cerevisiae) metabolites using in vitro and in vivo models

Chen,

Xiao,

Zhao

et al. 2024

Front. Microbiol.

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IntroductionYeast culture (YC) enhances ruminant performance, but its functional mechanism remains unclear because of the complex composition of YC and the uncertain substances affecting rumen fermentation. The objective of this study was to determine the composition of effective metabolites in YC by exploring its effects on rumen fermentation in vitro, growth and slaughter performance, serum index, rumen fermentation parameters, rumen microorganisms, and metabolites in lambs.MethodsIn Trial 1, various YCs were successfully produced, providing raw materials for identifying effective metabolites. The experiment was divided into 5 treatment groups with 5 replicates in each group: the control group (basal diet without additives) and YC groups were supplemented with 0.625‰ of four different yeast cultures, respectively (groups A, B, C, and D). Rumen fermentation parameters were determined at 3, 6, 12, and 24 h in vitro. A univariate regression model multiple factor associative effects index (MFAEI; y) was established to correlate the most influential factors on in vitro rumen fermentation with YC metabolites (x). This identified the metabolites promoting rumen fermentation and optimal YC substance levels. In Trial 2, metabolites in YC not positively correlated with MFAEI were excluded, and effective substances were combined with pure chemicals (M group). This experiment validated the effectiveness of YC metabolites in lamb production based on their impact on growth, slaughter performance, serum indices, rumen parameters, microorganisms, and metabolites. Thirty cross-generation rams (Small tail Han-yang ♀ × Australian white sheep ♂) with good body condition and similar body weight were divided into three treatment groups with 10 replicates in each group: control group, YC group, pure chemicals combination group (M group).ResultsGrowth performance and serum index were measured on days 30 and 60, and slaughter performance, rumen fermentation parameters, microorganisms, and metabolites were measured on day 60. The M group significantly increased the dressing percentage, and significantly decreased the GR values of lambs (p < 0.05). The concentration of growth hormone (GH), Cortisol, insulin (INS), and rumen VFA in the M group significantly increased (p < 0.05).DiscussionThese experiments confirmed that YC or its screened effective metabolites positively impact lamb slaughter performance, rumen fermentation, and microbial metabolism.

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“…Paper mulberry (Broussonetia papyrifera), a novel type of woody forage, possesses desirable characteristics such as strong stress resistance and high crude protein content (15-22% based on dry matter). Additionally, it contains abundant bioactive substances, including flavonoids [10,11]. Ensiling is commonly used for preserving fresh paper mulberry [12].…”

Section: Introductionmentioning

confidence: 99%

Dietary Paper Mulberry Silage Supplementation Improves the Growth Performance, Carcass Characteristics, and Meat Quality of Yangzhou Goose

Wang,

Xiong

et al. 2024

Animals

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There have been few investigations into the health benefits and meat quality of supplementing Yangzhou geese with paper mulberry silage. One hundred and twenty 28-day-old Yangzhou geese were selected for the experiment and randomly divided into two groups: a control group (CON) and a paper mulberry silage group (PM), with six replicates in each group. The experiment lasted for a total of 6 weeks. The experiment found that compared with CON, PM had a promoting effect on the average daily weight gain of Yangzhou geese (p = 0.056). Sensory and nutritional analysis of breast muscles revealed a decrease in a* value (p < 0.05) and an increase in protein content (p < 0.05) following PM treatment. Through untargeted metabolomics analysis of breast muscle samples, it was found that 11 different metabolites, including guanidinoacetic acid and other substances, had a positive effect on amino acid metabolism and lipid antioxidant pathways of PM treatment. Overall, the strategy of feeding Yangzhou geese with paper mulberry silage is feasible, which can improve the sensory quality and nutritional value of goose meat. The experiment provides basic data for the application form of goose breeding, so exploring the impact of substances within paper mulberry on goose meat should be focused on in the future.

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Exploring the rumen microbiota of Hu lambs in response to diet with paper mulberry

Cited by 5 publications

References 44 publications

Effect of Different Feeds on the Fungi Microbiome of Suffolk Crossed with Tibetan Sheep

Effect of Different Feeds on the Fungi Microbiome of Suffolk Crossed with Tibetan Sheep

Mechanistic insights into rumen function promotion through yeast culture (Saccharomyces cerevisiae) metabolites using in vitro and in vivo models

Dietary Paper Mulberry Silage Supplementation Improves the Growth Performance, Carcass Characteristics, and Meat Quality of Yangzhou Goose

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