Changes in the Proportion of Type I and Type III Collagen in the Developing and Retained Bovine Placentome

Sharpe, Kathy L.; Eiler, Hugo; Hopkins, Fred M.

doi:10.1095/biolreprod43.2.229

Cited by 25 publications

(16 citation statements)

References 30 publications

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“…In the bovine estrous cycle, fibronectin was distributed diffusely throughout the endometrial tissue, and integrin β1 subunit was present in the endometrial stroma [17]. The bovine placentome is composed of an interdigitation of cotyledonary and caruncular villi, and the rich vascular system and the majority of types I and III collagens are localized within the maternal villi [25]. The present study demonstrated that there was a parallel decrease in the expression of type I collagen and fibronectin during the early implantation period and that the feature of collagen fibers remarkably changed from thick and dense to thin and rough.…”

Section: Discussionmentioning

confidence: 99%

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The Dynamic Expression of Extracellular Matrix in the Bovine Endometrium at Implantation.

Yamada

Todoroki

Takahashi

et al. 2002

The Journal of Veterinary Medical Science

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ABSTRACT. Remodeling of uterine endometrial extracellular matrix (ECM) is pivotal to successful implantation and placentation, and has been well described in the rodents and humans. However, bovine endometrial ECM remodeling is still vaguely defined, especially at the time of implantation. Therefore, this study investigated the distribution of four ECMs namely, types I and IV collagen, laminin and fibronectin, from days 0 to 30 of gestation in bovine endometrium by immunofluorescence microscopy. A change in the distribution pattern of ECMs was evident by day 14 of gestation as features at this stage were clearly different from those of day 14 of the estrous cycle. The immunoreactivity of type I collagen, fibronectin and laminin decreased from day 14 of gestation and was obscured by day 24 of gestation. The type I collagen fibers formed were of thinner consistency than those of the estrous cycle and showed a coarser meshwork within the epithelium sites during the implantation period. In addition, the type IV collagen and laminin immunoreactivities of epithelial basement membrane also remarkably declined at exactly the same time. By day 30 of gestation, the four ECMs had regenerated with the formation of the placentome. In conclusion, this study reveals that remodeling of ECM is essential for the successful establishment of pregnancy in the bovine.

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Section: Discussionmentioning

confidence: 99%

“…The type IV collagen and laminin constitute customarily the basement membrane of epithelium, blood vascular vessels and various kinds of gland [5,25]. The structure of type IV collagen is expressed as a network of mesh sheets and serves as multiple cell-binding sites.…”

Section: Discussionmentioning

confidence: 99%

The Dynamic Expression of Extracellular Matrix in the Bovine Endometrium at Implantation.

Yamada

Todoroki

Takahashi

et al. 2002

The Journal of Veterinary Medical Science

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“…Se o sistema de ancoragem não é enzimaticamente degradado, as membranas fetais ficam retidas. Vacas com RP apresentam uma colagenólise diminuída (Gross et al 1985, Melendez et al 2006, ou ainda um sistema anti-colagenase mais ativo (Eiler & Hopkins 1993), sendo incapazes de degradar o colagénio tipo III, que diminui significativamente em vacas normais no final da gestação (Sharpe et al 1990).…”

Section: Discussionunclassified

Apoptose e maturação placentária bovina: um estudo imunohistoquímico e morfométrico

et al. 2016

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Placental release after birth involves loss of maternal-fetal adhesion and occurs only after complete maturation of the placentoma related to the decrease in cellularity of fetal and maternal tissues. Apoptosis is required for both the normal maturation and release of the placenta after birth. The aim of this study was to evaluate the occurrence of apoptosis in samples of the placenta of cows in different stages of gestation. Samples of 15 healthy cow placentomas at 4 (n=5), 6 (n=5) and 9 (n=5) months of gestation were harvested and processed for routine histology, immunohistochemistry and histochemistry. The slides were stained with HE, PicroSirius Red and subjected to immunohistochemical analysis of proteins Caspase 3, Caspase 8, Bax and Bid. Increase in number of vessels was not associated with increase in vascular area during progression of gestation. The results of histomorphometry revealed increased labeling for Bax and Caspases 3 and 8 in trophoblastic binucleated cells in late pregnancy, where the Bid remained without significant change. Histomorphometry analyzing the mononuclear trophoblast cells showed a high expression for Bax in early pregnancy, but decreased at 6 months of gestation. Immunolabeling revealed increased Caspases 3/8 and Bid with advancing of gestation. Further evaluation of type I and III collagen showed a decrease of both types of collagens at the end of gestation, what is very important for the reduction of maternal-fetal adhesion. These results confirm that Caspases 3 and 8 and Bax are involved in the mechanisms of activation of apoptosis through mitochondrial intrinsic and/or extrinsic pathway during pregnancy in trophoblastic binucleated cells. In mononuclear trophoblast cells Bax looses importance in the apoptosis process, awhile Bid and caspases 3 and 8 become the most significant.

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“…Microscopicamente, a grade contendo 25 pontos equidistantes foi sobreposta a 80 campos microscópicos escolhidos aleatoriamente, totalizando 2000 pontos por lâmina. O número de vezes em que os pontos atingiram uma determinada estrutura foi utilizado para calcular a proporção volumétrica dela, segundo a fórmula de Sharpe et al (1989).…”

Section: Methodsunclassified

Características histomorfométricas do placentomo de vacas Nelore nos terços inicial, médio e final de gestação

Machado¹,

Xavier²,

Júnior³

2010

Arq. Bras. Med. Vet. Zootec.

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RESUMOAvaliaram-se as características histomorfométricas do placentomo da vaca Nelore nos terços inicial, médio e final da gestação utilizando-se 52 úteros gestantes. Para a estimativa do estádio gestacional, foram utilizados aspectos do desenvolvimento fetal. Foram coletados fragmentos de um placentomo localizado no corno uterino ipsilateral ao corpo lúteo, os quais foram imersos em solução de formol e, posteriormente, processados histologicamente, incluídos em parafina e corados com hematoxilina-eosina. O estudo das proporções volumétricas foi feito utilizando-se microscópio de luz equipado com ocular integradora. Foi feita, também, a quantificação das células epiteliais nas criptas das carúnculas por meio de microscopia de luz e uma ocular micrométrica com 100 divisões. Foi verificado decréscimo no número das células maternas do epitélio das criptas no final da gestação. Os números médios das células binucleadas do trofoblasto, nos terços inicial, médio e final da gestação, mantiveram-se semelhantes, bem como a proporção volumétrica dos componentes dos tecidos conjuntivos materno e fetal e dos vasos no tecido materno. No tecido fetal, ocorreu aumento gradativo do tecido conjuntivo nos terços inicial, médio e final.Palavras-chave: vaca, Nelore, placentomo ABSTRACT Histomorphometric characteristics of the placentome of the Nelore cow in the initial

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Changes in the Proportion of Type I and Type III Collagen in the Developing and Retained Bovine Placentome

Cited by 25 publications

References 30 publications

The Dynamic Expression of Extracellular Matrix in the Bovine Endometrium at Implantation.

The Dynamic Expression of Extracellular Matrix in the Bovine Endometrium at Implantation.

Apoptose e maturação placentária bovina: um estudo imunohistoquímico e morfométrico

Características histomorfométricas do placentomo de vacas Nelore nos terços inicial, médio e final de gestação

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