Changes in the population of polyribosomal containing red cells of peripheral blood of rainbow trout, <i>Salmo gairdneri</i> Richardson, following starvation and bleeding

Lane, H. C.; Tharp, T. P.

doi:10.1111/j.1095-8649.1980.tb02743.x

Cited by 29 publications

(16 citation statements)

References 9 publications

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“…Their shape is less elyptic than that of mature cells, they have higher numbers of organelles and contain less haemoglobin (Lane and Tharp, 1980). During maturation their haemoglobin concentration increases and the organelles decrease in number (Keen et al, 1989).…”

Section: Discussionmentioning

confidence: 99%

Seasonal changes in blood cells of Arctic char (Salvelinus alpinus L.) from a high mountain lake

et al. 2000

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Seasonal changes in blood erythrocytes and lymphocytes were investigated in Arctic char from a remote high mountain lake at an altitude of 2344 m. Despite striking seasonal differences of environmental conditions the erythrocyte numbers remained constant during the year. Lymphocytes, however, displayed a distinct seasonal pattern with low abundance during the period of ice-cover (about 7 months) and high abundance in summer. During winter the circulating blood contained lymphocytes with damaged organelles and membranes, and almost exclusively mature red cells with a high density. With rising water temperature in July/August (11°C), however, high-density (old) cells almost disappeared, the proportion of immature cells dramatically increased, and the capacity to responding to handling stress decreased. We conclude that in summer the accelerated erythropoiesis (recruitment of red blood cells) compensates for the reduced life span of red blood cells.

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Section: Discussionmentioning

confidence: 99%

Seasonal changes in blood cells of Arctic char (Salvelinus alpinus L.) from a high mountain lake

et al. 2000

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“…The proportion of cells which contained this organelle (28.3%), located in fractions 6-10, was found to be the same proportion of polyribosomal-containing red cells in the whole blood of fed trout (Lane & Tharp, 1980). 1978) and by air-dried smears of enriched fractions of separated cells (Weaver, 1980).…”

Section: Recovery Rates and Cell Dimensionsmentioning

confidence: 95%

“…The separated cell fractions were pelleted and resuspended in 2Yo agar, solidified rapidly in a refrigerator and processed for transmission electron microscopy as described previously (Lane & Tharp, 1980).…”

Section: Electron Microscopymentioning

confidence: 99%

Some age related changes of adult rainbow trout, Salmo gairdneri Rich., peripheral erythrocytes separated by velocity sedimentation at unit gravity

Lane

Weaver

Benson

et al. 1982

Journal of Fish Biology

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Velocity sedimentation at unit gravity separated peripheral erythrocytes of adult rainbow trout according to age and size. The cells located at the top of the gradient contained numerous polyribosomes, few mitochondria and an essentially electronlucent nucleus. The cells at the bottom of the gradient no longer contained these cytoplasmic organelles and had an essentially electron-dense nucleus. The middle of the gradient contained cells with either very few polyribosomes or cells devoid of this organelle.The length, width and mean corpuscular volume (MCV) ofthe average cell increased and the width to length ratio decreased progressively from the top of the gradient to the bottom.No statistical significance could be determined in the changes of the mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) although they appeared to follow theoretical projections.The use of haematocrit, red cell count, haemoglobin concentration, MCV, MCH and MCHC in assessing erythropoietic activity and the size and haemoglobin content of the maturing cell is discussed.

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“…Indeed, fish erythrocytes have been demonstrated to possess the complete cellular machinery with functional ribosomes [9] and mitochondria [10-13], thus allowing protein synthesis and full cellular activity [14]. In amphibians, mature erythrocytes retain occasionally a few mitochondria, which are often of aberrant morphology [15] even if there is some functional evidence of their presence [16].…”

Section: Introductionmentioning

confidence: 99%

Avian erythrocytes have functional mitochondria, opening novel perspectives for birds as animal models in the study of ageing

et al. 2013

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BackgroundIn contrast to mammalian erythrocytes, which have lost their nucleus and mitochondria during maturation, the erythrocytes of almost all other vertebrate species are nucleated throughout their lifespan. Little research has been done however to test for the presence and functionality of mitochondria in these cells, especially for birds. Here, we investigated those two points in erythrocytes of one common avian model: the zebra finch (Taeniopygia guttata).ResultsTransmission electron microscopy showed the presence of mitochondria in erythrocytes of this small passerine bird, especially after removal of haemoglobin interferences. High-resolution respirometry revealed increased or decreased rates of oxygen consumption by erythrocytes in response to the addition of respiratory chain substrates or inhibitors, respectively. Fluorometric assays confirmed the production of mitochondrial superoxide by avian erythrocytes. Interestingly, measurements of plasmatic oxidative markers indicated lower oxidative stress in blood of the zebra finch compared to a size-matched mammalian model, the mouse.ConclusionsAltogether, those findings demonstrate that avian erythrocytes possess functional mitochondria in terms of respiratory activities and reactive oxygen species (ROS) production. Interestingly, since blood oxidative stress was lower for our avian model compared to a size-matched mammalian, our results also challenge the idea that mitochondrial ROS production could have been one actor leading to this loss during the course of evolution. Opportunities to assess mitochondrial functioning in avian erythrocytes open new perspectives in the use of birds as models for longitudinal studies of ageing via lifelong blood sampling of the same subjects.

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Changes in the population of polyribosomal containing red cells of peripheral blood of rainbow trout, Salmo gairdneri Richardson, following starvation and bleeding

Cited by 29 publications

References 9 publications

Seasonal changes in blood cells of Arctic char (Salvelinus alpinus L.) from a high mountain lake

Seasonal changes in blood cells of Arctic char (Salvelinus alpinus L.) from a high mountain lake

Some age related changes of adult rainbow trout, Salmo gairdneri Rich., peripheral erythrocytes separated by velocity sedimentation at unit gravity

Avian erythrocytes have functional mitochondria, opening novel perspectives for birds as animal models in the study of ageing

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