2000
DOI: 10.1046/j.1365-2672.2000.01183.x
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Changes in the Lactobacillus community during Ricotta forte cheese natural fermentation

Abstract: The loss of microbial biodiversity due to the increase in large‐scale industrial processes led to the study of the natural microflora present in a typical little known dairy product. The community of lactobacilli was studied in order to understand the natural fermentation of Ricotta forte cheese. The combined use of RAPD analysis, 16S rDNA sequencing and physiological tests allowed 33 different strains belonging to 10 species of Lactobacillus to be characterized. RAPD analysis revealed the heterogeneity of bot… Show more

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Cited by 71 publications
(54 citation statements)
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“…Over 90% of the bacteria found were facultatively heterofermentative lactobacilli. This confirms the results found with other cheese types [1,8,14,16] and indicates that these mesophilic lactobacilli can contribute to the flavour of Gruyère cheese.…”
Section: Cheesesupporting
confidence: 91%
“…Over 90% of the bacteria found were facultatively heterofermentative lactobacilli. This confirms the results found with other cheese types [1,8,14,16] and indicates that these mesophilic lactobacilli can contribute to the flavour of Gruyère cheese.…”
Section: Cheesesupporting
confidence: 91%
“…The bacterial colonies were analyzed by using the two-step randomly amplified polymorphic DNA (RAPD)-PCR protocol, as described elsewhere (2), with three different primers: Lac1, 5Ј-AGCAGCGTGG-3Ј; M 13, 5Ј-GAGGGTGG CGGTTCT-3Ј; and Ope 7, 5Ј-AGATGCAGCC-3Ј.…”
Section: Methodsmentioning
confidence: 99%
“…paraplantarum, these two closely related species belonging to the Lb. plantarum group [12]. Chen et al [33] analysed the 5S-23S rRNA intergenic spacer regions (ISRs) of the Lactobacillus group.…”
Section: Sequencingmentioning
confidence: 99%
“…Some of the primers used are listed in Table VI. Randomly amplified polymorphic DNA analysis has been used to monitor population dynamics in food fermentation and to estimate the diversity of Lactobacillus strains in cheeses [12,17,22] whey culture [38], sausage fermentation [137,152] and maize dough [91]. It can also be used to distinguish a particular strain from the natural flora, such as distinguishing Lactobacillus probiotic adjunct from the natural NSLAB population in Cheddar cheese [172].…”
Section: Rapd/ Ap-pcrmentioning
confidence: 99%