Changes in the expression of estrogen receptor mRNA in the utero-vaginal junction containing sperm storage tubules in laying hens after repeated artificial insemination

Das, S. C.; Nagasaka, Naohiro; Yoshimura, Y.

doi:10.1016/j.theriogenology.2005.07.004

Cited by 18 publications

(12 citation statements)

References 19 publications

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“…In addition, these authors also noted that the mucosa surrounding the SST possessed increased numbers of antigen-presenting cells expressing MHC class II, CD4 + , and CD8 + T phenotypes (Das et al, 2005a). Concurrent with the increased numbers of these immunocompetent cells and the abnormal appearance of the SST was a decrease in the abundance of mRNA for estrogen receptor-α (Das et al, 2006b). Their observations prompted the suggestion that because SST structure and function is estrogen dependent, the decreased mRNA expression of estrogen receptor-α, coupled with the increased numbers of immunocompetent cells in and around the SST, may be directly related to the absence of significant sperm storage within the SST in low fertility hens.…”

Section: Immunological Aspects Of Sperm Selection and Storagementioning

confidence: 90%

PHYSIOLOGY AND ENDOCRINOLOGY SYMPOSIUM: Role of the oviduct in maintaining sustained fertility in hens1

Bakst

2011

Journal of Animal Science

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Section: Immunological Aspects Of Sperm Selection and Storagementioning

confidence: 90%

PHYSIOLOGY AND ENDOCRINOLOGY SYMPOSIUM: Role of the oviduct in maintaining sustained fertility in hens1

Bakst

2011

Journal of Animal Science

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“…Nowadays, the chicken ( Gallus domesticus ) and the Guinea fowl ( Numida meleagris ) are important sources of eggs and meat. However, the intensification of the poultry industry has increased the interest in artificial insemination (AI) techniques and, therefore, the sperm fertilizing capacity of these species 34…”

Section: Introductionmentioning

confidence: 99%

“…In addition to this, lack of knowledge, age, housing, breeding management, artificial incubation, nutrition, photoperiod, sex determination, environment, and many other factors severely limit the reproductive capacity of these avian species 12345. Thus, semen quality and quantity could be the important limiting factors for an AI program, especially because all efforts related to the increase in reproductive performance should be focused on improving reproductive ability 6.…”

Section: Introductionmentioning

confidence: 99%

Sperm subpopulations in avian species: a comparative study between the rooster (Gallus domesticus) and Guinea fowl (Numida meleagris)

García-Herreros

2016

Asian J Androl

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The main aims of this research were to study possible differences in objective morphometric sperm characteristics, establish normative sperm morphometry standards, and evaluate the presumed different subpopulation distribution of avian spermatozoa from the rooster (Gallus domesticus) and Guinea fowl (Numida meleagris) as model avian species. Seventy-two ejaculates (36 per species studied) were obtained manually, following a training period involving gently combined dorso-abdominal and lumbo-sacral massage of the birds. Ejaculates were processed for volume, sperm concentration, viability, motility, and morphology. Moreover, samples were submitted for sperm morphometric assessment using objective Computer-Assisted Semen Analysis for Morphometry (CASA-Morph) methods, with sperm morphometric descriptors evaluated by Principal Component Analysis (PCA) and multivariate clustering analyses. There were several differences observed between the avian species in values obtained for ejaculate volume and sperm concentration (P < 0.001). Irrespective of species, PCA revealed two Principal Components (PCs) explaining more than 80% of the variance. In addition, the number of subpopulations differed with species (three and five subpopulations for rooster and Guinea fowl, respectively). Moreover, the distribution of the sperm subpopulations was found to be structurally different between species. In conclusion, our findings from using CASA-Morph methods indicate pronounced sperm morphometric variation between these two avian species. Because of the strong differences observed in morphometric parameter values and their subpopulation distribution, these results suggest that application of objective analytical methods such as CASA-Morph could substantially improve the reliability of comparative studies and help establish valid normative sperm morphological values for avian species.

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“…The SST are formed by the invaginations of mucosal surface, where sperm are found to be close contact with the epithelium (Ashizawa & Nishiyama 1983). A number of studies have examined the features of sperm survivability in SST, and suggested that quiescence of spermatozoa during the storage (Holm et al 2000), interaction of SST fluid outflow from SST cells and sperm mobility (Froman 2003, Zaniboni & Bakst 2004, and protection of sperm from immunoreaction (Das et al 2005a(Das et al , 2006 may be important events in this process. However, the precise mechanism of sperm survivability in the SST has not been established.…”

Section: Introductionmentioning

confidence: 99%

Expression of transforming growth factor-β isoforms and their receptors in utero-vaginal junction of hen oviduct in presence or absence of resident sperm with reference to sperm storage

Das

Isobe²,

Nishibori³

et al. 2006

Reproduction

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Our goal was to determine whether transforming growth factor b (TGFb) isoforms were involved in the process of sperm survivability in the sperm-storage tubules (SST) in the utero-vaginal junction (UVJ) of hen oviduct. The birds were artificially inseminated. The mRNA expressions of three types of TGFb isoforms (TGFb2, TGFb3, and TGFb4) and three types of receptors (TbR1, TbR2, and TbR3) were examined in the presence or in the absence of resident sperm in SST by semi-quantitative reverse transcriptase-PCR. The mRNA expression of TGFbs and TbRs in sperm was also examined. Immunocytochemistry and western blot were performed for TbR2 to confirm its localization in UVJ. The sperm were observed at least 10 days after insemination by histology. The mRNA expressions of TGFbs and TbRs were significantly increased in UVJ in the presence of resident sperm in SST. The mRNA expressions of TGFbs and TbRs were also observed in sperm. Immunohistochemistry revealed that TbR2 were located in lymphocytes in UVJ and SST cells. The presence of TbR2 in UVJ was also confirmed by western blot. These results suggest that enhanced expressions of TGFbs and TbRs in UVJ may protect sperm in SST, probably by suppressing anti-sperm immunoreactions.

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Changes in the expression of estrogen receptor mRNA in the utero-vaginal junction containing sperm storage tubules in laying hens after repeated artificial insemination

Cited by 18 publications

References 19 publications

PHYSIOLOGY AND ENDOCRINOLOGY SYMPOSIUM: Role of the oviduct in maintaining sustained fertility in hens1

PHYSIOLOGY AND ENDOCRINOLOGY SYMPOSIUM: Role of the oviduct in maintaining sustained fertility in hens1

Sperm subpopulations in avian species: a comparative study between the rooster (Gallus domesticus) and Guinea fowl (Numida meleagris)

Expression of transforming growth factor-β isoforms and their receptors in utero-vaginal junction of hen oviduct in presence or absence of resident sperm with reference to sperm storage

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