Expression of transforming growth factor-β isoforms and their receptors in utero-vaginal junction of hen oviduct in presence or absence of resident sperm with reference to sperm storage

Das, S. C.; Isobe, Naoki; Nishibori, Masahide; Yoshimura, Y.

doi:10.1530/rep.1.01177

Cited by 65 publications

(62 citation statements)

References 43 publications

(30 reference statements)

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“…Mallmann and Diedrich [33] and Lu et al [34] demonstrated that the female sperm allergy is an important cause leading to the problem of sperm survival, this allergy was present in the infertile groups. Normal sperm survival and fertility may be maintained by suppression of antisperm immunoreactions through TGF-beta and its receptor (accession no: NM_001031045 and NM_205428) in the UV junction [35,36]. All of these immunoreactions may indicate that an allergic response of an EVL-mediated T-cell reaction happened in the birds from the short-fertileperiod group and that sperm cannot survive in the UV junction for a long period of time.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Transcript Expression Analysis in the Uterovaginal Junction in Association with Fertile Period in Tsaiya Ducks

Huang

Cheng

Yang

et al. 2011

Journal of Reproduction and Development

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Abstract. We performed the first genome-wide expression analysis to compare the differences in gene expression in the female sperm reservoir of the duck reproductive tract between two groups with long and short fertile periods to identify factors that may be associated with the fertile period using an oligonucleotide microarray. RNA was extracted from the uterovaginal junction (UV junction) of the two groups. Affymetrix chips containing comprehensive coverage of 32773 transcripts were hybridized with biotin-labeled cRNA, and three biological repeats were performed. We identified 27 transcripts as being differentially regulated. Interestingly, by mapping the differentially expressed transcripts to annotated pathways, we found that Neuropeptide Y (NPY), the RNA expression of which was increased by 2.96-fold in the short-fertile-period group as compared with the long-fertile-period group in our experiment, has been shown to reduce blood flow and substance supply to local tissues. Enah/Vasp-like (EVL), the RNA expression of which was significantly increased by 1.77-fold in the short-fertile-period group as compared with the long-period group, has been demonstrated to be important in activated T-cells. In contrast, trafficking kinesin-binding protein 1 (TRAK1), the expression of which was increased by 2.33-fold in the long-period group as compared with its counterparts, has been suggested to inhibit precocious activation of sperm and prolong sperm life in the female sperm reservoir. The results of real-time PCR confirmed the data obtained by microarray analysis. Our study demonstrated that combining global gene expression investigation with annotated pathway resources contributes to the understanding of sperm life when sustained in the UV junction.Key words: Differentially expressed transcripts, Duck, Fertile period, Uterovaginal junction (J. Reprod. Dev. 57: [731][732][733][734][735][736] 2011) A fter the entry of sperm into a female genital tract before ovulation, sperm accumulate to a certain amount and are stored in the female sperm reservoir [1], which is a widespread phenomenon among numerous avian, reptilian and mammalian species [2][3][4]. The sperm reservoir then releases sperm gradually for successful fertilization after a single insemination, the period of which varies from hours to weeks in different species [2,[4][5][6]. This period is defined as the fertile period. A long fertile period means prolonged sperm survival and leads to a high fertilization rate [7].The process of sperm migration in the female genital tract is more than simply transportation from the position of insemination to the position of fertilization. Rather, it is a complex continuum: entry into the lower genital tract, defense against the tract environment and immunological response, retention in the storage reservoir, activation, taxiing of activated sperm towards oocytes [8], and phagocytosis of nonfertilizing sperm. During sperm storage in the reservoir of the female reproductive tract, there is a close interaction between sperm...

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Transcript Expression Analysis in the Uterovaginal Junction in Association with Fertile Period in Tsaiya Ducks

Huang

Cheng

Yang

et al. 2011

Journal of Reproduction and Development

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“…Their observations prompted the suggestion that because SST structure and function is estrogen dependent, the decreased mRNA expression of estrogen receptor-α, coupled with the increased numbers of immunocompetent cells in and around the SST, may be directly related to the absence of significant sperm storage within the SST in low fertility hens. These authors also observed that transforming growth factor-β (TGF-β) and their receptors (TβR) increased in the UVJ when sperm reside in the SST (Das et al, 2006a). Given the immunosuppressive properties of TGF-β, and that lymphocytes in the UVJ mucosa possess TβR, Das et al (2006a) suggested that TGF-β may suppress immune responses to resident sperm in the SST by UVJ lymphocytes.…”

Section: Immunological Aspects Of Sperm Selection and Storagementioning

confidence: 99%

“…These authors also observed that transforming growth factor-β (TGF-β) and their receptors (TβR) increased in the UVJ when sperm reside in the SST (Das et al, 2006a). Given the immunosuppressive properties of TGF-β, and that lymphocytes in the UVJ mucosa possess TβR, Das et al (2006a) suggested that TGF-β may suppress immune responses to resident sperm in the SST by UVJ lymphocytes. Das et al (2008) concluded that this suppression of UVJ lymphocytes by TGF-β may contribute to successful sperm storage in the SST.…”

Section: Immunological Aspects Of Sperm Selection and Storagementioning

confidence: 99%

PHYSIOLOGY AND ENDOCRINOLOGY SYMPOSIUM: Role of the oviduct in maintaining sustained fertility in hens1

Bakst

2011

Journal of Animal Science

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“…To determine sperm migration in oviducts, Das et al studied the rate of SST filling after a single insemination event in chickens. Sperm artificially introduced into the vagina of a chicken reached the SSTs within an hour and the rate of SST filling tended to increase until 24 h after insemination (Das et al 2006). Although the mechanisms underlying the process of sperm selection are not known, the intrinsic mobility of sperm may be an important factor in the adovarian transport of sperm in the vagina and the uptake of sperm by the SSTs (Froman 2003).…”

Section: Discussionmentioning

confidence: 99%

Sperm activation by heat shock protein 70 supports the migration of sperm released from sperm storage tubules in Japanese quail (Coturnix japonica)

Hiyama¹,

Matsuzaki²,

Mizushima³

et al. 2014

Reproduction

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Systems for maintaining the viability of ejaculated sperm in the female reproductive tract are widespread among vertebrates and invertebrates. In birds, this sperm storage function is performed by specialized simple tubular invaginations called sperm storage tubules (SSTs) in the uterovaginal junction (UVJ) of the oviduct. Although the incidence and physiological reasons for sperm storage in birds have been reported extensively, the mechanisms of sperm uptake by the SSTs, sperm maintenance within the SSTs, and control of sperm release from the SSTs are poorly understood. In this study, we demonstrated that the highly conserved heat shock protein 70 (HSP70) stimulates sperm motility in vitro and also that HSP70 expressed in the UVJ may facilitate the migration of sperm released from the SSTs.Quantitative RT-PCR analysis demonstrated that the expression of HSP70 mRNA in the UVJ increases before ovulation/oviposition. Gene-specific in situ hybridization and immunohistochemical analysis with a specific antibody to HSP70 demonstrated that HSP70 is localized in the surface epithelium of the UVJ. Furthermore, injection of anti-HSP70 antibody into the vagina significantly inhibited fertilization in vivo. In addition, we found that recombinant HSP70 activates flagellar movement in the sperm and that the binding of recombinant HSP70 to the sperm surface is mediated through an interaction with voltage-dependent anion channel protein 2 (VDAC2). Our results suggest that HSP70 binds to the sperm surface by interacting with VDAC2 and activating sperm motility. This binding appears to play an important role in sperm migration within the oviduct.

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Expression of transforming growth factor-β isoforms and their receptors in utero-vaginal junction of hen oviduct in presence or absence of resident sperm with reference to sperm storage

Cited by 65 publications

References 43 publications

Genome-Wide Transcript Expression Analysis in the Uterovaginal Junction in Association with Fertile Period in Tsaiya Ducks

Genome-Wide Transcript Expression Analysis in the Uterovaginal Junction in Association with Fertile Period in Tsaiya Ducks

PHYSIOLOGY AND ENDOCRINOLOGY SYMPOSIUM: Role of the oviduct in maintaining sustained fertility in hens1

Sperm activation by heat shock protein 70 supports the migration of sperm released from sperm storage tubules in Japanese quail (Coturnix japonica)

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