Changes in the Canine Plasma Lipidome after Short- and Long-Term Excess Glucocorticoid Exposure

Sieber-Ruckstuhl, Nadja S; Burla, Bo; Spoerel, Susanne; Schmid, Florence; Venzin, Claudio; Cazenave-Gassiot, Amaury; Bendt, Anne K.; Torta, Federico; Wenk, Markus R.; Boretti, Felicitas S

doi:10.1038/s41598-019-42190-1

Cited by 19 publications

(32 citation statements)

References 63 publications

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“…Lipids were extracted using a single-phase, single-step liquid–liquid extraction method with butanol:methanol as previously described (Alshehry et al 2015; Sieber-Ruckstuh et al 2019). Serum samples were thawed on ice and 10 µL aliquots were each mixed with 90 µL extraction mix, containing 1-butanol:methanol (1:1, v:v) and the internal standards (ISTDs): Cer d18:1/17:0 (Avanti Polar Lipids, 860517P), DG 12:0_12:0 (Avanti 800812P), LPC 20:0 (Avanti 855777P), LPE 14:0 (Avanti 856735P), PC 14:0/14:0 (Avanti 850345P), PE 14:0/14:0 (Avanti 850745P), PG 14:0/14:0 (Avanti 840445), PS 14:0/14:0 (Avanti 840033P), SM d18:1/C12:0 (Avanti 860583P), TAG 16:0_16:0_16:0 d5 (CDN Isotopes, D-5815), LacCer d18:1/16:0 d3 (Matreya LLC, 1533).…”

Section: Methodsmentioning

confidence: 99%

“…In veterinary medicine as well, the S1P pathway has been suggested as a target in studying the pathogenesis of certain diseases and in developing new therapeutic interventions (Nakazawa et al 2019; Rodriguez et al 2015). Administration of glucocorticoids has been shown to induce S1P in dogs (Sieber-Ruckstuh et al 2019). The various influences, however, of diets on S1P have not yet been evaluated in veterinary medicine.…”

Section: Introductionmentioning

confidence: 99%

“…Knowledge about the plasma or serum lipidome, S1P and the influence of diets and diet composition on the lipidomic profile in dogs is only sparse (Sieber-Ruckstuh et al 2019; Lloyd et al 2017; Yin et al 2012). One study evaluated inter-breed signatures in the plasma lipidomic profiles of dogs (Lloyd et al 2017).…”

Section: Introductionmentioning

confidence: 99%

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Serum lipidome analysis of healthy beagle dogs receiving different diets

et al. 2019

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IntroductionFood and dietary ingredients have significant effects on metabolism and health.ObjectiveTo evaluate whether and how different diets affected the serum lipidomic profile of dogs.MethodsSixteen healthy beagles were fed a commercial dry diet for 3 months (control diet). After an overnight fasting period, a blood sample was taken for serum lipidomic profile analysis, and each dog was then randomly assigned to one of two groups. Group 1 was fed a commercial diet (Diet 1) and group 2 was fed a self-made, balanced diet supplemented with linseed oil and salmon oil (Diet 2) for 3 months. After an overnight fasting period, a blood sample was taken from each dog. Serum cholesterol and triacylglycerol analyses were performed and the serum lipidomic profiles were analyzed using targeted liquid chromatography–mass spectrometry.ResultsDogs fed the supplemented self-made diet (Diet 2) had significantly higher omega-3 fatty acid-containing lipids species and significantly lower saturated and mono- and di-unsaturated lipid species. Concentrations of sphingosine 1-phosphate species S1P d16:1 and S1P d17:1 were significantly increased after feeding Diet 2.ConclusionThis study found that different diets had significant effects on the dog’s serum lipidomic profile. Therefore, in studies that include lipidomic analyses, diet should be included as a confounding factor.Electronic supplementary materialThe online version of this article (10.1007/s11306-019-1621-3) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Serum lipidome analysis of healthy beagle dogs receiving different diets

et al. 2019

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“…Furthermore, patient-specific variability should be raised and controlled instantly in the study design step, including, but not limited to, age, gender, ethnicity, body mass index, diet, circadian rhythms, smoking habits, physical activity, health status, and concomitant medications [64]. Taken as an example, excess exposure to glucocorticoids, which are drugs that are strictly associated with metabolic control, may affect plasma lipidome [65]. Experts in metabolomics and QA/QC procedures gathered in a meeting entitled "Think Tank on Quality Assurance and Quality Control for Untargeted Metabolomic Studies" to identify appropriate reporting standards of QA/QC activities for publications and databases, leading to the foundation of the metabolomics Quality Assurance and Quality Control Consortium [62,66].…”

Section: The High Demand For Quality Assurance and Quality Control Stmentioning

confidence: 99%

Toward a Standardized Strategy of Clinical Metabolomics for the Advancement of Precision Medicine

et al. 2020

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Despite the tremendous success, pitfalls have been observed in every step of a clinical metabolomics workflow, which impedes the internal validity of the study. Furthermore, the demand for logistics, instrumentations, and computational resources for metabolic phenotyping studies has far exceeded our expectations. In this conceptual review, we will cover inclusive barriers of a metabolomics-based clinical study and suggest potential solutions in the hope of enhancing study robustness, usability, and transferability. The importance of quality assurance and quality control procedures is discussed, followed by a practical rule containing five phases, including two additional “pre-pre-” and “post-post-” analytical steps. Besides, we will elucidate the potential involvement of machine learning and demonstrate that the need for automated data mining algorithms to improve the quality of future research is undeniable. Consequently, we propose a comprehensive metabolomics framework, along with an appropriate checklist refined from current guidelines and our previously published assessment, in the attempt to accurately translate achievements in metabolomics into clinical and epidemiological research. Furthermore, the integration of multifaceted multi-omics approaches with metabolomics as the pillar member is in urgent need. When combining with other social or nutritional factors, we can gather complete omics profiles for a particular disease. Our discussion reflects the current obstacles and potential solutions toward the progressing trend of utilizing metabolomics in clinical research to create the next-generation healthcare system.

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“…By these premises, we considered whether analogous data are reported in domestic animals, finding only a few studies on plasma or other tissues (brain, sperm) and on correlations between dietary uptake and lipidomic profiles of dogs (30)(31)(32)(33)(34). Limited studies presented a small number of samples (35,36), focusing for example on PUFA (omega-6 and omega-3), and not deepening the different fatty acid families, which are instead important for molecular contribution to membrane homeostasis (37).…”

Section: Introductionmentioning

confidence: 99%

The Erythrocyte Membrane Lipidome of Healthy Dogs: Creating a Benchmark of Fatty Acid Distribution and Interval Values

et al. 2020

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Molecular-based approaches are rapidly developing in medicine for the evaluation of physiological and pathological conditions and discovery of new biomarkers in prevention and therapy. Fatty acid diversity and roles in health and disease in humans are topical subjects of lipidomics. In particular, membrane fatty acid-based lipidomics provides molecular data of relevance in the study of human chronic diseases, connecting metabolic, and nutritional aspects to health conditions. In veterinary medicine, membrane lipidomics, and fatty acid profiles have not been developed yet in nutritional approaches to health and in disease conditions. Using a protocol widely tested in human profiling, in the present study erythrocyte membrane lipidome was examined in 68 clinically healthy dogs, with different ages, sex, and sizes. In particular, a cluster composed of 10 fatty acids, present in membrane glycerophospholipids and representative of structural and functional properties of cell membrane, was chosen, and quantitatively analyzed. The interval values and distribution for each fatty acid of the cluster were determined, providing the first panel describing the healthy dog lipidomic membrane profile, with interesting correlation to bodyweight increases. This molecular information can be advantageously developed as benchmark in veterinary medicine for the evaluation of metabolic and nutritional status in healthy and diseased dogs.

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Changes in the Canine Plasma Lipidome after Short- and Long-Term Excess Glucocorticoid Exposure

Cited by 19 publications

References 63 publications

Serum lipidome analysis of healthy beagle dogs receiving different diets

Serum lipidome analysis of healthy beagle dogs receiving different diets

Toward a Standardized Strategy of Clinical Metabolomics for the Advancement of Precision Medicine

The Erythrocyte Membrane Lipidome of Healthy Dogs: Creating a Benchmark of Fatty Acid Distribution and Interval Values

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