Changes in the activities of enzymes of kidney gluconeogenesis during development of the rat

Hauser, Christine A.; Bailey, E.

doi:10.1016/0020-711x(75)90046-4

Cited by 6 publications

(8 citation statements)

References 12 publications

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“…Clearly, these hormones are unlikely to influence renal PEP carboxykinase activity. It is possible that the response of renal PEP carboxykinase to cAMP is age-dependent and Hommes and Beere [16] have shown that cAMP levels in kidney rise at 5-10 days post partum which is also the period of the second developmental increase in kidney PEP carboxykinase [14], However, the results presented in this paper suggest that cAMP does not affect the activity of PEP carboxy kinase in the kidney of the rat at any age. cAMP has been shown to increase glucose formation in kidney cortex slices [22], isolated perfused kidney [4] and kidney cortex tubules [12,25].…”

Section: Discussioncontrasting

confidence: 55%

“…Gluconeogenesis does take place to some extent in fetal kidney and extramitochondrial PEP carboxykinase activity is present in the late fetal kidney [14]. The activity of the enzyme increases rapidly at birth, remains constant until about 10 days of age and then increases rapidly again [14].…”

Section: Introductionmentioning

confidence: 98%

“…Liver and kidneys were removed from experimental animals and tissue homogenates prepared and fractionated for PEP carboxykinase assay as described by Hauser and Bailey [14]. PEP carboxykinase activity was assayed as described by Ballard and Hanson [2], Enzyme activities are expressed as units/g wet weight of tissue, 1 unit being defined as 1 [xmole of substrate transformed per minute at 25 °C.…”

Section: Methodsmentioning

confidence: 99%

“…The activity of the enzyme increases rapidly at birth, remains constant until about 10 days of age and then increases rapidly again [14]. Nothing is known concerning the factors involved in the two marked in creases in renal PEP carboxykinase activity during development.…”

Section: Introductionmentioning

confidence: 99%

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Hormonal Control of Renal Phosphoenolpyruvate Carboxykinase Activity during Development of the Rat

Hauser¹,

Bailey²

1976

Enzyme

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The effect of injections of hormones in utero on fetal rat kidney and liver extramitochondrial phosphoenolpyruvate carboxykinase activity has been studied. Glucagon and thyroxine induced the liver enzyme but none of the hormones tested affected the renal enzyme. In the postnatal rat, the hepatic phosphoenolpyruvate carboxykinase activity is increased after triamcinolone or thyroxine injection but only triamcinolone injection increases the activity of the kidney enzyme. It is suggested that the rise in renal phosphoenolpyruvate carboxykinase activity at about 10 days of age is due to the increase in blood corticosterone content occurring at the same age.

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Section: Discussioncontrasting

confidence: 55%

Section: Introductionmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Hormonal Control of Renal Phosphoenolpyruvate Carboxykinase Activity during Development of the Rat

Hauser¹,

Bailey²

1976

Enzyme

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“…These results suggest that there may be a shift in relative importance of lipid and carbohydrate degradation in the liver during the mid-suckling period despite the continued intake of a high-fatcontent diet. It is noteworthy that at this time there is also an increase in renal gluconeogenesis (Hauser & Bailey, 1975). With regard to acylcarnitine oxidation (P. C. Foster & E. Bailey, unpublished results), it should be noted that the rates obtained are at all ages considerably lower than are the activities of any of the enzymes reported here, e.g.…”

Section: Resultsmentioning

confidence: 55%

Changes in the activities of the enzymes of hepatic fatty acid oxidation during development of the rat

Foster

Bailey

1976

Biochemical Journal

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1. Changes in the activities of several enzymes involved in mitochondrial fatty acid oxidation were measured in the livers of developing rats between late foetal life and maturity. The enzymes studied are medium-and long-chain ATP-dependent acyl-CoA synthetases of the outer mitochondrial membrane and matrix, GTP-dependent acyl-CoA synthetase, camitine acyltransferase, enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, general 3-oxoacyl-CoA thiolase and acetoacetyl-CoA thiolase. Developmental changes in the activity of cytoplasmic acetoacetyl-CoA thiolase were also investigated. 2. The activities of all the mitochondrial enzymes studied increase markedly at birth and, except for enoyl-CoA hydratase, decrease at weaning. However, all the developmental patterns other than those of enoyl-CoA hydratase and acetoacetyl-CoA thiolase exhibit a fall followed by a rise in enzyme activity during the mid-suckling period. 3. The cytoplasmic acetoacetyl-CoA thiolase activity is low throughout suckling, but high before birth and after weaning. 4. The results are discussed in relation to changes in fatty acid supply to the liver and hormonal changes occurring during development.

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Development of ammonia and glucose productions from glutamine in foetal rat kidney; Effects of metabolic acidosis

1979

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Ammoniagenesis and gluconeogenesis have been studied in foetal rat kidneys during the five last days of gestation by measuring in vitro NH3 and glucose productions from glutamine and by assaying activities of soluble phosphoenolpyruvate carboxykinase(PEPCK) and glucose 6-phosphatase (G6Pase). These studies were carried out in normal (mean blood pH: 7.30) and acidotic (pH: 7.12) foetuses. In normal foetuses, NH3 production by kidney cortex slices remains constant throughout the studied period of development, at a level 10-fold lower than the maternal one. On day 20 of gestation, a low glucose production (20-fold lower than the maternal one) appears for the first time. This may be related to an increase of PEPCK and G6Pase activities which occurs between day 19 and 20. In 20 days old foetuses, NH4Cl induced acidosis stimulates NH3 production but has no effect on PEPCK activity and glucose production. A response of gluconeogenesis to acidosis is observed one day later (day 21).

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Changes in the activities of enzymes of kidney gluconeogenesis during development of the rat

Cited by 6 publications

References 12 publications

Hormonal Control of Renal Phosphoenolpyruvate Carboxykinase Activity during Development of the Rat

Hormonal Control of Renal Phosphoenolpyruvate Carboxykinase Activity during Development of the Rat

Changes in the activities of the enzymes of hepatic fatty acid oxidation during development of the rat

Development of ammonia and glucose productions from glutamine in foetal rat kidney; Effects of metabolic acidosis

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