Changes in Some Biochemical
 Parameters of Mulberry (Morus sp.)
 Leaves after Infected with Leaf Spot Disease

Ghosh, Lipika; Alam, M. S.; Ali, Muhammad; Shohael, Abdullah Mohammad; Alam, F.; Islam, Rafiqul

doi:10.3923/jbs.2003.508.514

Cited by 8 publications

(1 citation statement)

References 3 publications

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“…Pectin, a natural polymer, accounts for 4.6% of mulberry leaf (Ghosh et al, 2003). Its digestion in the silkworm digestive system, like cellulose digestion, it also been driven by pectinase synthesised by the microbial community (Taylor, 1982).…”

Section: Resultsmentioning

confidence: 99%

Effect of spirulina fortification on the digestive protease, carbohydrases and cocoons economic traits of mulberry silkworm Bombyx mori

2023

Int.J.Zool.Appl.Biosci

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Bombyx mori, the silkworm, is a voracious feeder that relies on digestive enzymes for mulberry digestion and its conversion in to silk. In the current study, fifth-instar larvae were given 2% spirulina supplementation with commercially graded spirulina samples, and a few digestive enzymes and cocoon traits were examined. The treatment groups of silkworms T1-control, T2-supplemented with spirulina powder graded for animal dietary consumption, T3- graded for human dietary consumption and T4-spirulina graded for medicinal applications. Spirulina supplementation was tested in silkworms for digestive protease, carbohydrases and cocoon traits. The results shows that the total protein content percentage difference (PD) in the gut wall was increased by 2.79% in T3, 1.96% in T4, and 0.78% in T2 compared to T1 (control). A similar trend was noticed in the gut content, with the PD of 15.81% in T3, 7.51% in T4, and 3.95% in T2. In total carbohydrate content, observed PD of 16.35% in T2, 11.27% in T3, and 9.38% in T4, while the gut content was 13% both in T2 and T3, and 1.22% in T4. The protease activity PD of gut wall tissue has 21.99% in T3, 5.13% in T2, and 4.30% in T4; similarly, the gut content has a PD of 112.38% in T3, 106.12% in T4, and 86.41% in T2. The amylase activity have shown highest PD in T4 (33.43%), T3 (23.37%), and moderate in T2 (19.23%) in the gut wall, and 93.10% in T3, 66.44% in T2, and 14.92% in T4 of gut content. Enzymes such as sucrase, cellulase, and pectinase have also improved with spirulina fortification. The sucrase activity in the gut wall and gut content were, respectively, 7.99% and 80.73% in T3 treatment. Noteworthy, the T2 showed 37.69% in the gut wall and 24.54% in the gut content for the cellulase activity. Unlike cellulase, pectinase activity has been observed with a PD of 93.15% in the gut wall and 64.63% in gut content. The study further confirms that the additional fortification of spirulina will have a beneficial effect on the digestive cells of the silkworm, Bombyx mori, resulting in the highest rate of enzyme activity and in turn, increasing survivability.

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Section: Resultsmentioning

confidence: 99%

Effect of spirulina fortification on the digestive protease, carbohydrases and cocoons economic traits of mulberry silkworm Bombyx mori

2023

Int.J.Zool.Appl.Biosci

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Synthesis and Toxicity of Silver Nanoparticles

Pandiarajan¹,

Balaji²,

Mahendran³

et al. 2016

Sustainable Agriculture Reviews

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Isolation and Characterization of Bacteria from the Gut ofBombyx morithat Degrade Cellulose, Xylan, Pectin and Starch and Their Impact on Digestion

Anand

Vennison

Sankar

et al. 2010

Journal of Insect Science

275

191

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Bombyx mori L. (Lepidoptera: Bombycidae) have been domesticated and widely used for silk production. It feeds on mulberry leaves. Mulberry leaves are mainly composed of pectin, xylan, cellulose and starch. Some of the digestive enzymes that degrade these carbohydrates might be produced by gut bacteria. Eleven isolates were obtained from the digestive tract of B. mori, including the Gram positive Bacillus circulans and Gram negative Proteus vulgaris, Klebsiella pneumoniae, Escherichia coli, Citrobacter freundii, Serratia liquefaciens, Enterobacter sp., Pseudomonas fluorescens, P. aeruginosa, Aeromonas sp., and Erwinia sp.. Three of these isolates, P. vulgaris, K. pneumoniae, C. freundii, were cellulolytic and xylanolytic, P. fluorescens and Erwinia sp., were pectinolytic and K. pneumoniae degraded starch. Aeromonas sp. was able to utilize the CMcellulose and xylan. S. liquefaciens was able to utilize three polysaccharides including CMcellulose, xylan and pectin. B. circulans was able to utilize all four polysaccharides with different efficacy. The gut of B. mori has an alkaline pH and all of the isolated bacterial strains were found to grow and degrade polysaccharides at alkaline pH. The number of cellulolytic bacteria increases with each instar.

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Changes in Some Biochemical Parameters of Mulberry (Morus sp.) Leaves after Infected with Leaf Spot Disease

Cited by 8 publications

References 3 publications

Effect of spirulina fortification on the digestive protease, carbohydrases and cocoons economic traits of mulberry silkworm Bombyx mori

Effect of spirulina fortification on the digestive protease, carbohydrases and cocoons economic traits of mulberry silkworm Bombyx mori

Synthesis and Toxicity of Silver Nanoparticles

Isolation and Characterization of Bacteria from the Gut ofBombyx morithat Degrade Cellulose, Xylan, Pectin and Starch and Their Impact on Digestion

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