Changes in rumen fermentation and bacterial profiles after administering Lactiplantibacillus plantarum as a probiotic

Abstract

Background and Aim: Lactiplantibacillus plantarum is one of the lactic acid bacteria that is often used as probiotics. This study aimed to evaluate the effects of Lactiplantibacillus plantarum TSD10 as a probiotic on rumen fermentation and microbial population in Ongole breed cattle. Materials and Methods: This study adopted an experimental crossover design, using three-fistulated Ongole breed cattle. Treatments were as follows: T0, control without probiotic; T1, 10 mL probiotic/day; T2, 20 mL probiotic/day; and T3, 30 mL probiotic/day. The basal diet of the cattle comprised 70% concentrate: 30% elephant grass (Pennisetum purpureum). The concentration of probiotic used was 1.8 × 1010 colony-forming unit (CFU)/mL. Results: We observed significantly lower acetate production compared with control (64.12%), the lowest values being in the T3 group (55.53%). Contrarily, propionate production significantly increased from 18.67% (control) to 23.32% (T2). All treatments yielded significantly lower acetate–propionate ratios than control (3.44), with the lowest ratio in the T3 group (2.41). The protozoal number decreased on probiotic supplementation, with the lowest population recorded in the T2 group (5.65 log cells/mL). The population of specific rumen bacteria was estimated using a quantitative polymerase chain reaction. We found that the population of L. plantarum, Ruminococcus flavefaciens, and Treponema bryantii, did not change significantly on probiotic supplementation, While that of Ruminococcus albus increased significantly from 9.88 log CFU/mL in controls to 12.62 log CFU/mL in the T2 group. Conclusion: This study showed that the optimum dosage of L. plantarum TSD10 as a probiotic was 20 mL/day. The effect of L. plantarum as a probiotic on feed degradation in rumen was not evaluated in this experiment. Therefore, the effect of L. plantarum as a probiotic on feed degradation should be performed in further studies.

Lactic acid bacteria and yeast strains isolated from fermented fish (Budu) identified as candidate ruminant probiotics based on in vitro rumen fermentation characteristics

“…Astuti et al . [ 15 ] demonstrated that increased feed digestibility can confirm that lactic acid bacteria could be applied as a probiotic by stimulating the activity of rumen bacteria. Improved digestibility indicates that supplementing lactic acid bacteria can stimulate fibrolytic bacteria in the rumen [ 39 ].…”

“…Probiotics, especially the beneficial bacteria and yeast, can restore the microbial balance of the digestive tract and against pathogenic bacteria [ 14 ]. Lactic acid bacteria can interact with the rumen microorganisms, increase propionate and total volatile fatty acid (VFA) production, enhance feed efficiency and growth performance, and reduce methane gas production [ 15 ] and the incidence of diarrhea [ 16 ]. Meanwhile, yeast supplementation in livestock rations can increase productivity, health, use of cellulose material, and reproduction [ 17 , 18 ].…”

New probiotics (Lactobacillus plantarum and Saccharomyces cerevisiae) supplemented to fermented rice straw-based rations on digestibility and rumen characteristics in vitro

“…Among the commonly used probiotics are Lactobacillus plantarum (lactic acid bacteria; LAB) and Saccharomyces cerevisiae (yeast). LAB as probiotics can increase feed efficiency, interact with rumen microorganisms, increase propionate production and total volatile fatty acid (VFA) [ 12 ], and have the potential to act as growth promoters [ 10 ]. Supplementation of S. cerevisiae can increase the use of cellulose (CLD), consume oxygen, and prevent the overproduction of lactic acid [ 13 ].…”