Changes in Renal Tissue Proteome Induced by Mesenteric Lymph Drainage in Rats After Hemorrhagic Shock With Resuscitation

Zhao, Zi‐Gang; Zhang, Limin; Lv, Yong-Zhuang; Si, Yong-Hua; Niu, Chun‐Yu; Li, Jicheng

doi:10.1097/shk.0000000000000214

Cited by 9 publications

(4 citation statements)

References 32 publications

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“…During sepsis, refractory hypotension, extensive microthrombus, abnormal hemodynamics, and microcirculatory disturbance lead to organ dysfunction and damage and/or death (Angus and van der Poll, 2013; Maloney, 2013). Numerous studies have shown that pre-treatment or post-administration of resveratrol (Res) could alleviate organ injury in animal models of endotoxic shock or sepsis induced by cecal ligation and puncture (Kolgazi et al, 2006), or administration of lipopolysaccharide (LPS) by either intravenous injection (Sebai et al, 2011), intraperitoneal injection (Zhao et al, 2014), or airway instilment (Knobloch et al, 2011). These beneficial effects of Res treatment are associated with anti-inflammatory response and relief from oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

Effect of Resveratrol on Blood Rheological Properties in LPS-Challenged Rats

et al. 2018

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Objectives: Abnormal rheological properties induce adverse effects during sepsis. This study sought to investigate the hypothesis that resveratrol (Res) improves blood rheological properties in rats following a lipopolysaccharide (LPS) challenge, and provide a novel approach for treatment of sepsis.Methods: The rats were intraperitoneally or intramuscularly injected with vehicle, LPS (8 mg/kg), Res (30 mg/kg), or both to yield four groups: control, Res, LPS, and LPS + Res. After 6 h of LPS and/or Res injection, the mean arterial pressure (MAP), regional blood flow, erythrocyte and leukocyte parameters, and blood viscosity were observed.Results: LPS administration had no significant effects on the erythrocyte parameters and plasma viscosity. LPS administration reduced the MAP, whole blood viscosity at low and medium shear rates, the blood flow in the spleen and kidney, and the leukocyte content in whole blood when compared to control group, and increased the myeloperoxidase (MPO) activity in lung. Treatment with Res alone had no effects on most of parameters observed except increasing the whole blood relative viscosity. However, Res treatment after LPS resulted in further decrease in whole blood viscosity at high and medium shear rates. Furthermore, Res treatment conversely decreased the red blood cell distribution width-CV, blood flow of stomach, whole blood relative viscosity and MPO activity in lung, and increased the leukocyte content, but did not restore LPS-induced decrease in MAP and the blood flow in the spleen and kidney.Conclusion: The Res treatment partly reduce the whole blood viscosity and regional blood flow, and increase WBC content in peripheral blood following the LPS challenge, suggesting a favorable role in expanding the quasi-sympathetic effects of LPS in blood viscosity at early stages.

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Section: Introductionmentioning

confidence: 99%

Effect of Resveratrol on Blood Rheological Properties in LPS-Challenged Rats

et al. 2018

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“…Twelve animals were used to establish a model of hemorrhagic shock model (40 mm Hg for 90 min, followed by resuscitation with shed whole blood and equal Ringer solution) under anesthesia, and mesenteric lymph was drained after fluid resuscitation for 3 h and collected under anesthesia as previously described (17,18). To confirm the intestinal lymphatic mechanism of E2 therapy on hemorrhagic shock-induced ALI, partial rats with acute hemorrhage received subcutaneous injection of E2 (2 mg/kg) upon resuscitation.…”

Section: Posthemorrhagic Shock Mesenteric Lymph Collection From Anest...mentioning

confidence: 99%

Estrogen Alleviates Posthemorrhagic Shock Mesenteric Lymph-Mediated Lung Injury Through Autophagy Inhibition

Sun

Zhang

et al. 2023

Shock

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Background: Hemorrhagic shock–induced acute lung injury (ALI) is commonly associated with the posthemorrhagic shock mesenteric lymph (PHSML) return. Whether excessive autophagy is involved in PHSML-mediated ALI remains unclear. The relationship between estrogen treatment and PHSML or autophagy needs to verify. The current study will clarify the role of estrogen in reducing PHSML-mediated ALI through inhibition of autophagy. Methods: First, a hemorrhagic shock model in conscious rats was used to observe the effects of 17β-estradiol (E2) on intestinal blood flow, pulmonary function, intestinal and pulmonary morphology, and expression of autophagy marker proteins. Meanwhile, the effect of PHSML and autophagy agonist during E2 treatment was also investigated. Secondly, rat primary pulmonary microvascular endothelial cells were used to observe the effect of PHSML, PHSML plus E2, and E2-PHSML (PHSML obtained from rats treated by E2) on the cell viability. Results: Hemorrhagic shock induced intestinal and pulmonary tissue damage and increased wet/dry ratio, reduced intestinal blood flow, along with pulmonary dysfunction characterized by increased functional residual capacity and lung resistance and decreased inspiratory capacity and peak expiratory flow. Hemorrhagic shock also enhanced the autophagy levels in intestinal and pulmonary tissue, which was characterized by increased expressions of LC3 II/I and Beclin-1 and decreased expression of p62. E2 treatment significantly attenuated these adverse changes after hemorrhagic shock, which was reversed by PHSML or rapamycin administration. Importantly, PHSML incubation decreased the viability of pulmonary microvascular endothelial cells, while E2 coincubation or E2-treated lymph counteracted the adverse roles of PHSML. Conclusions: The role of estrogen reducing PHSML-mediated ALI is associated with the inhibition of autophagy.

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“…Under inhalation anesthesia with 2% isoflurane (Shenzhen Ruiwald Life Technology Co., Ltd., Shenzhen, China) by the inhalation anesthesia machine for small animals (MATRX VMR; Midmark Corporation, Dayton, OH), before the surgical and hemorrhage procedures, either the authentic or sham SGB procedures were performed according to previous reports, with minor alterations (12,(15)(16)(17). After hemorrhagic shock (40 mm Hg for 90 min, followed by resuscitation with shed whole blood and equal Ringer's solution), the PHSML and PHSML-SGB were harvested from rats under anesthetic conditions (18)(19)(20).…”

Section: Experimental Design and Protocolsmentioning

confidence: 99%

STELLATE GANGLION BLOCK REVERSES PHSML-INDUCED VASCULAR HYPOREACTIVITY THROUGH INHIBITING AUTOPHAGY-MEDIATED PHENOTYPIC TRANSFORMATION OF VSMCs

Li,

Du,

Zhao

et al. 2023

Shock

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Post-hemorrhagic shock mesenteric lymph (PHSML) return-contributed excessive autophagy of vascular smooth muscle cells (VSMCs) is involved in vascular hypo-reactivity, which is inhibited by stellate ganglion block (SGB) treatment. The contractile phenotype of VSMCs transforms into a synthetic phenotype after stimulation with excessive autophagy. Therefore, we hypothesized that SGB ameliorates PHSML-induced vascular hypo-reactivity by inhibiting autophagy-mediated phenotypic transformation of VSMCs. To substantiate this hypothesis, a hemorrhagic shock model in conscious rats was employed to observe the effects of SGB intervention or intravenous infusion of the autophagy inhibitor 3-MA on intestinal blood flow and the expression of autophagy- and phenotype-defining proteins in mesenteric secondary artery tissues. We also investigated the effects of intraperitoneal administration of PHSML intravenous infusion and the autophagy agonist rapamycin (RAPA) on the beneficial effect of SGB. The results showed that hemorrhagic shock decreased intestinal blood flow and enhanced the expression of LC3 II/I, Beclin1, and matrix metalloproteinase 2, which were reversed by SGB or 3-MA treatment. In contrast, RAPA and PHSML administration abolished the beneficial effects of SGB. Furthermore, the effects of PHSML or PHSML obtained from rats treated with SGB (PHSML-SGB) on cellular contractility, autophagy, and VSMC phenotype were explored. Meanwhile, the effects of 3-MA on PHSML and RAPA on PHSML-SGB were observed. The results showed that PHSML, but not PHSML-SGB, incubation decreased VSMCs contractility and induced autophagy activation and phenotype transformation. Importantly, 3-MA administration reversed the adverse effects of PHSML, and RAPA treatment attenuated the effects of PHSML-SGB incubation on VSMCs. Taken together, the protective effect of SGB on vascular reactivity is achieved by inhibiting excessive autophagy-mediated phenotypic transformation of VSMCs to maintain their contractile phenotype.

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Changes in Renal Tissue Proteome Induced by Mesenteric Lymph Drainage in Rats After Hemorrhagic Shock With Resuscitation

Cited by 9 publications

References 32 publications

Effect of Resveratrol on Blood Rheological Properties in LPS-Challenged Rats

Effect of Resveratrol on Blood Rheological Properties in LPS-Challenged Rats

Estrogen Alleviates Posthemorrhagic Shock Mesenteric Lymph-Mediated Lung Injury Through Autophagy Inhibition

STELLATE GANGLION BLOCK REVERSES PHSML-INDUCED VASCULAR HYPOREACTIVITY THROUGH INHIBITING AUTOPHAGY-MEDIATED PHENOTYPIC TRANSFORMATION OF VSMCs

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