Changes in red blood cell membrane structure in type 2 diabetes: a scanning electron and atomic force microscopy study

Buys, Antoinette V.; Rooy, Mia-Jeanne van; Soma, Prashilla; Papendorp, Dirk van; Lipiński, Bogusław; Pretorius, Etheresia

doi:10.1186/1475-2840-12-25

Cited by 166 publications

(133 citation statements)

References 14 publications

(25 reference statements)

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“…The surface roughness was computed from the AFM height images of the local areas. The cell membrane roughness has been shown to be a biomarker in the cellular physiological activity (e.g., the membrane roughness of red blood cells indicates the disease status of diabetes patients 39 and cell membrane roughness can identify oxidative stressinduced cellular apoptosis 40 and also the expression of tumor suppressor protein 41 ). Hence, investigating cell membrane roughness is useful for understanding the cell biological activity.…”

Section: Resultsmentioning

confidence: 99%

Nanoscale Imaging and Mechanical Analysis of Fc Receptor-Mediated Macrophage Phagocytosis against Cancer Cells

Liu

et al. 2014

Langmuir

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Fc receptor-mediated macrophage phagocytosis against cancer cells is an important mechanism in the immune therapy of cancers. Traditional research about macrophage phagocytosis was based on optical microscopy, which cannot reveal detailed information because of the 200-nm-resolution limit. Quantitatively investigating the macrophage phagocytosis at micro-and nanoscale levels is still scarce. The advent of atomic force microscopy (AFM) offers an excellent analytical instrument for quantitatively investigating the biological processes at single-cell and singlemolecule levels under native conditions. In this work, we combined AFM and fluorescence microscopy to visualize and quantify the detailed changes in cell morphology and mechanical properties during the process of Fc receptor-mediated macrophage phagocytosis against cancer cells. Lymphoma cells were discernible by fluorescence staining. Then, the dynamic process of phagocytosis was observed by time-lapse optical microscopy. Next, AFM was applied to investigate the detailed cellular behaviors during macrophage phagocytosis under the guidance of fluorescence recognition. AFM imaging revealed the distinct features in cellular ultramicrostructures for the different steps of macrophage phagocytosis. AFM cell mechanical property measurements indicated that the binding of cancer cells to macrophages could make macrophages become stiffer. The experimental results provide novel insights in understanding the Fc-receptor-mediated macrophage phagocytosis.

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Section: Resultsmentioning

confidence: 99%

Nanoscale Imaging and Mechanical Analysis of Fc Receptor-Mediated Macrophage Phagocytosis against Cancer Cells

Liu

et al. 2014

Langmuir

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“…Here, the Laser-Assisted Optical Rotational Red Cell Analyzer (LORRCA) [455][456][457][458] (marketed by Mechatronics) provides a convenient measurement, while atomic force microscopy assesses deformability at the level of the single cell. [459][460][461][462][463] Similar comments apply to the changes in red cell distribution width also commonly seen in inflammatory diseases (e.g. ref.…”

Section: Effects Of Non-ionic Substancesmentioning

confidence: 99%

The simultaneous occurrence of both hypercoagulability and hypofibrinolysis in blood and serum during systemic inflammation, and the roles of iron and fibrin(ogen)

Kell

Pretorius

2014

Integrative Biology

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Although the two phenomena are usually studied separately, we summarise a considerable body of literature to the effect that a great many diseases involve (or are accompanied by) both an increased tendency for blood to clot (hypercoagulability) and the resistance of the clots so formed (hypofibrinolysis) to the typical, 'healthy' or physiological lysis. We concentrate here on the terminal stages of fibrin formation from fibrinogen, as catalysed by thrombin. Hypercoagulability goes hand in hand with inflammation, and is strongly influenced by the fibrinogen concentration (and vice versa); this can be mediated via interleukin-6. Poorly liganded iron is a significant feature of inflammatory diseases, and hypofibrinolysis may change as a result of changes in the structure and morphology of the clot, which may be mimicked in vitro, and may be caused in vivo, by the presence of unliganded iron interacting with fibrin(ogen) during clot formation. Many of these phenomena are probably caused by electrostatic changes in the iron-fibrinogen system, though hydroxyl radical (OH ) formation can also contribute under both acute and (more especially) chronic conditions. Many substances are known to affect the nature of fibrin polymerised from fibrinogen, such that this might be seen as a kind of bellwether for human or plasma health. Overall, our analysis demonstrates the commonalities underpinning a variety of pathologies as seen in both hypercoagulability and hypofibrinolysis, and offers opportunities for both diagnostics and therapies.

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“…[1][2][3][4] Pathological alterations in RBC deformability have been associated with various diseases 5 such as malaria, 6,7 sickle cell anemia, 8 diabetes, 9 hereditary disorders, 10 myocardial infarction, 11 and paroxysmal nocturnal hemoglobinuria (PNH). 12 Because of its pathophysiological importance, measurement of RBC deformability has been the focus of numerous studies over the past decades.…”

Section: Introductionmentioning

confidence: 99%

Biomechanical properties of red blood cells in health and disease towards microfluidics

2014

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Red blood cells (RBCs) possess a unique capacity for undergoing cellular deformation to navigate across various human microcirculation vessels, enabling them to pass through capillaries that are smaller than their diameter and to carry out their role as gas carriers between blood and tissues. Since there is growing evidence that red blood cell deformability is impaired in some pathological conditions, measurement of RBC deformability has been the focus of numerous studies over the past decades. Nevertheless, reports on healthy and pathological RBCs are currently limited and, in many cases, are not expressed in terms of well-defined cell membrane parameters such as elasticity and viscosity. Hence, it is often difficult to integrate these results into the basic understanding of RBC behaviour, as well as into clinical applications. The aim of this review is to summarize currently available reports on RBC deformability and to highlight its association with various human diseases such as hereditary disorders (e.g., spherocytosis, elliptocytosis, ovalocytosis, and stomatocytosis), metabolic disorders (e.g., diabetes, hypercholesterolemia, obesity), adenosine triphosphate-induced membrane changes, oxidative stress, and paroxysmal nocturnal hemoglobinuria. Microfluidic techniques have been identified as the key to develop state-of-the-art dynamic experimental models for elucidating the significance of RBC membrane alterations in pathological conditions and the role that such alterations play in the microvasculature flow dynamics. V C 2014 AIP Publishing LLC.

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Changes in red blood cell membrane structure in type 2 diabetes: a scanning electron and atomic force microscopy study

Cited by 166 publications

References 14 publications

Nanoscale Imaging and Mechanical Analysis of Fc Receptor-Mediated Macrophage Phagocytosis against Cancer Cells

Nanoscale Imaging and Mechanical Analysis of Fc Receptor-Mediated Macrophage Phagocytosis against Cancer Cells

The simultaneous occurrence of both hypercoagulability and hypofibrinolysis in blood and serum during systemic inflammation, and the roles of iron and fibrin(ogen)

Biomechanical properties of red blood cells in health and disease towards microfluidics

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