Nanoscale Imaging and Mechanical Analysis of Fc Receptor-Mediated Macrophage Phagocytosis against Cancer Cells

Li, Mi; Liu, Lianqing; Xi, Ning; Wang, Yuechao; Xiao, Xiubin; Zhang, Weijing

doi:10.1021/la4042524

Cited by 45 publications

(34 citation statements)

References 64 publications

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“…To date, a challenge to this type of research has been posed by the occasional difficulty of exactly discerning one specific type of cell from others when only cellular Young's modulus is used (Li et al, 2015). When researchers have used AFM to quantify the Young's modulus of diverse types of cells, the overlap in Young's modulus among them (Kuznetsova et al, 2007;Yu et al, 2011;Li et al, 2014a;Hayashi and Iwata, 2015) has proved to be an important issue. Our results, as seen in Figure 5, show that cellular relaxation time can be a useful complement to discern different types of cells when they display considerable overlap in cellular Young's modulus.…”

Section: Rusults and Discussionmentioning

confidence: 99%

Atomic force microscopy studies on cellular elastic and viscoelastic properties

Liu

et al. 2017

Sci. China Life Sci.

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In this work, a method based on atomic force microscopy (AFM) approach-reside-retract experiments was established to simultaneously quantify the elastic and viscoelastic properties of single cells. First, the elastic and viscoelastic properties of normal breast cells and cancerous breast cells were measured, showing significant differences in Young's modulus and relaxation times between normal and cancerous breast cells. Remarkable differences in cellular topography between normal and cancerous breast cells were also revealed by AFM imaging. Next, the elastic and viscoelasitc properties of three other types of cell lines and primary normal B lymphocytes were measured; results demonstrated the potential of cellular viscoelastic properties in complementing cellular Young's modulus for discerning different states of cells. This research provides a novel way to quantify the mechanical properties of cells by AFM, which allows investigation of the biomechanical behaviors of single cells from multiple aspects.

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Section: Rusults and Discussionmentioning

confidence: 99%

Atomic force microscopy studies on cellular elastic and viscoelastic properties

Liu

et al. 2017

Sci. China Life Sci.

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“…Much more systematic experimental and theoretical work should be investigated in this area. (iii) The enclosed environment for ODEP-based cell manipulations restricts direct scanning of cell membrane surface by cell patch clamp [126] or AFM [127]. The combination of an OEK device and a traditional microfluidic chip provides a possible way to solve this problem.…”

Section: Discussionmentioning

confidence: 99%

Micro and Nano Manipulation and Assembly by Optically Induced Electrokinetics

Lai

et al. 2015

Micro‐ and Nanomanipulation Tools

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IntroductionIn the past 25 years, integrated circuit (IC) and microelectromechanical systems (MEMSs) technologies have moved much beyond the microdomain, and into the submicro-, nanoscopic, and, even, the molecular scales. Today, we have the capability to fabricate, manipulate, and assemble matters at the micro-, nano-, or molecular scales. This progress into "seeing" and manipulating matters that are smaller than visible light wavelength scale is impacting a range of fields including semiconductor physics, biological studies, pharmaceutical development, and many other scientific and technological applications. A range of new methods to generate physical forces have been developed in the process. For example, mechanical forces can now be applied to micro-, nano-, and biological entities using atomic force microscope probes [1], microgrippers [2], or pipettes [3]. However, disadvantages of these approaches, including inflection of mechanical damages on objects being manipulated and their relatively low manipulation speed, make manipulation of large quantities of objects difficult. In order to address these disadvantages, noncontact or noninvasive methods for micro-, nano-, and biological manipulation have also been explored in the past two decades.Manipulation devices based on magnetic forces [4], acoustic waves [5,6], hydrodynamic flows [7], light waves [8], or electrokinetic forces [9] are among the major noninvasive technologies available today. Each of them has its own advantages and disadvantages. Methods based on magnetic fields need premodification on objects with magnetic beads which constrains their application domain. In addition, movement controlled by permanent magnets is not precise enough for many proposed applications. Also, the high resistance of the magnetic coil generates significant heat during manipulation [10]. Acoustic traps show good selectivity but cannot achieve parallel manipulation of single entities. Microfluidic devices exploiting hydrodynamic flows are suitable for cell population sorting, but have difficulties in trapping or controlling specific single objects.Electrokinetic forces generated from patterned electrodes capable of inducing the desired spatial distribution of electric field have been exploited to control

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“…There has been a tendency within the field to believe that NK cells are the most potent effectors according to the results in vitro, but some scholars have proposed that perhaps in vivo macrophages are much more important [7]. In order to explore the nanoscale situations taking place in phagocytosis, we have used AFM to visualize the quantification of the dynamical changes in cellular nanostructures and mechanical properties during the process of rituximab-mediated macrophage phagocytosis against lymphoma cells [32], as shown in Fig. 4.…”

Section: Adcc Mechanismmentioning

confidence: 99%

“…Traditional studies about the rituximab's ADCC mechanisms were based on ensemble results from many cells Fig. 4 Changes of cellular nanostructures and mechanical properties during the ADCC mechanism (macrophage phagocytosis) of rituximab revealed by AFM [32]. a-f AFM deflection images of a, c, e whole cells and b, d, f local areas (denoted by the squares) during the macrophage phagocytosis.…”

Section: Adcc Mechanismmentioning

confidence: 99%

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Applications of Atomic Force Microscopy in Exploring Drug Actions in Lymphoma-Targeted Therapy at the Nanoscale

Liu

et al. 2015

BioNanoSci.

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Rituximab, a monoclonal antibody against the CD20 molecule expressed on B cells, has revolutionized the treatment of B cell lymphomas. The use of rituximab significantly improves the long-term survival rates of lymphoma patients. However, there are still many patients who develop resistance to rituximab. Hence, the urgent need is enhancing the potency of anti-CD20 antibodies beyond that achieved with rituximab to provide effective therapies for more patients. Traditional studies about the killing mechanisms of rituximab are commonly based on optical microscopy, which cannot reveal the detailed situations due to the limit of 200-nm resolution. Quantitatively investigating the actions of rituximab at the nanoscale is still scarce. The advent of atomic force microscopy (AFM) provides a powerful and versatile platform for in situ investigating the nanoscale behaviors of individual live cells. The applications of AFM in life sciences in the past decade have yielded a lot of novel insights into cell biology and related diseases. In this article, the typical applications (e.g., ultra-microstructures, mechanical properties, and molecular recognition) of AFM in investigating the three killing mechanisms of rituximab at the nanoscale are summarized; the challenges facing AFM single-cell assay and its future directions are also discussed.

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Nanoscale Imaging and Mechanical Analysis of Fc Receptor-Mediated Macrophage Phagocytosis against Cancer Cells

Cited by 45 publications

References 64 publications

Atomic force microscopy studies on cellular elastic and viscoelastic properties

Atomic force microscopy studies on cellular elastic and viscoelastic properties

Micro and Nano Manipulation and Assembly by Optically Induced Electrokinetics

Applications of Atomic Force Microscopy in Exploring Drug Actions in Lymphoma-Targeted Therapy at the Nanoscale

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