Changes in protein profile detected in seedlings of Caesalpinia peltophoroides (Fabaceae) after exposure to high concentration of cadmium

Abstract

ABSTRACT. Sibipiruna (Caesalpinia peltophoroides Benth) is a tree of the Brazilian Atlantic Forest. It is a flowering ornamental tree widely planted throughout Brazil and indicated for restoration of degraded areas. We examined protein profile changes in leaves of seedlings of C. peltophoroides grown in nutrient solution under greenhouse conditions, after exposure to cadmium (Cd; 32 mg/L). A two-dimensional gel was used to analyze proteins expressed in response to stress 24 and 72 h after initiation of treatment with Cd. Various protein bands were identified that were related to stress response and/or metabolic adjustments, including proteins involved with resistance to stress, including detoxification, degradation, antioxidant, transport, signal transduction, photosynthesis, electron transport, biosynthesis reactions, and ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 11 (3): 2694-2707 (2012) Effect of cadmium on protein profile of C. peltophoroides 2695 transcription regulation. After 24 h of Cd exposure, the genes of most of these proteins were upregulated. These putative proteins were associated with resistance to stress, including heat shock proteins, heat stress transcriptional factor and other transcriptional factors, aquaporins, glutathione transferase and choline monooxygenase. Most of the putative proteins observed after 72 h of exposure to Cd were downregulated. They were mainly photosynthetic process proteins, such as NAD(P)H-quinone oxidoreductase, photosystem I assembly, and photosystem II CP47 chlorophyll apoprotein. There were also proteins involved with degradation, biosynthesis and antioxidant activity, such as ATP-dependent Clp protease, methylthioribose-1-phosphate and glutathione peroxidase 2. Based on preliminary proteomic analysis, we conclude that proteins related to photosynthetic activity are inhibited, decreasing plant performance under stress conditions and that several proteins related to defense mechanisms are activated, inducing the plant defense response.

Morphological, biochemical, molecular and ultrastructural changes induced by Cd toxicity in seedlings of Theobroma cacao L.

“…For living in environments contaminated with toxic metals, some plant species have developed survival strategies such as the exclusion of metals through the selective absorption of mineral elements; chelation of toxic metal ions by means of a specific high affinity ligand (Patra et al, 2004); accumulation of phytochelatins and metallothioneins ( [Pietrini et al, 2010,Schützendübel andPolle, 2002]); retention of toxic metals in the roots, preventing its translocation to the aerial part; compartmentalization of toxic metals for vacuoles, and adsorption to the cell wall; activation of antioxidant enzymes; differential expression of proteins (Gomes et al, 2012); exclusion and accumulation of large amounts of toxic metals in their tissues; and cellular repair mechanisms .…”

Electrophoresis and spectrometric analyses of adaptation-related proteins in thermally stressed Chromobacterium violaceum

“…Many of the stress responses overlap with one another in bacteria (Ishibashi et al, 2010) and adaptive reactions involve general changes in metabolism as well as in gene transcript levels (Jozefczuk et al, 2010;Gomes et al, 2012). The expression of heat shock genes in Gram-negative proteobacteria is positively modulated by the transcriptional regulator RpoH, the σ 32 subunit of RNA polymerase, which induces the expression of proteins involved in the response to heat and cold stress (Yura et al, 1993;El-Samad et al, 2005).…”