Changes in poly(A) tail length of maternal transcripts during in vitro maturation of bovine oocytes and their relation with developmental competence

Brevini, Tiziana A. L.; Favetta, Laura A.; Mauri, Luca; Luciano, A.M.; Cillo, F.; Gandolfi, F.

doi:10.1002/(sici)1098-2795(199904)52:4<427::aid-mrd12>3.0.co;2-g

Cited by 106 publications

(79 citation statements)

References 39 publications

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“…In the course of bovine IVM, the length of poly-(A) tails for cyclins B1 and B2 were reported to increase (Lastro et al, 1999). In our study, polyadenylation levels peaked in meiotically arrested oocytes, and showed decreases after IVM, which is consistent with a shortening of poly-(A) tails as reported with most genes analyzed in oocytes matured in vitro (Brevini-Gandolfi et al, 1999). These findings are in contrast with a recently described poly-(A) mRNA accumulation until metaphase II (Tomek et al, 2002) and with no apparent changes in poly-A-polymerase transcripts abundance during IVM .…”

Section: Discussionsupporting

confidence: 92%

Retinoid‐dependent mRNA expression and poly‐(A) contents in bovine oocytes meiotically arrested and/or matured in vitro

Gómez

Rodríguez

Dd́ez

et al. 2004

Molecular Reproduction Devel

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The presence of retinoic acid (RA) during in vitro maturation (IVM) improves bovine oocyte quality and developmental potential. In this work, we investigated the underlying molecular mechanisms. Cumulus-oocyte complexes were meiotically arrested by roscovitine and/or matured in defined medium containing RA, 1% ethanol (vehicle), or no additives. Cumulus-free oocytes were analyzed for poly-(A) mRNA contents and relative mRNA expression of genes involved in cell cycle regulation (cyclin B1 and H1) and antioxidative defence (Mn-superoxide dismutase and glucose-6-phosphate dehydrogenase). Poly-(A) mRNA increased after meiotic inhibition and decreased with IVM completion, both in meiotically arrested and permissively matured oocytes, i.e., matured without previous meiotic arrest. RA dramatically increased poly-(A) mRNA in meiotically arrested oocytes, but more than half of the poly-(A) mRNA disappeared during maturation. Irrespective of oocyte origin, transcripts were detected for all the genes analyzed. IVM, with or without previous meiotic inhibition, increased expression of cyclin B1 and glucose-6-phosphate dehydrogenase, and decreased cyclin H1 and Mn-superoxide dismutase. Except for a decreasing of Mn-superoxide dismutase in meiotically arrested and matured oocytes, RA did not affect mRNA expression. Ethanol led to an abnormal poly-(A) mRNA profile and expression of all the genes analyzed. RA does not modify expression of cyclin B1 and HI genes in the bovine oocyte, and probably does not generate oxidative stress. In addition, RA enhanced mRNA amount as measured by poly-(A) mRNA contents.

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Section: Discussionsupporting

confidence: 92%

Retinoid‐dependent mRNA expression and poly‐(A) contents in bovine oocytes meiotically arrested and/or matured in vitro

Gómez

Rodríguez

Dd́ez

et al. 2004

Molecular Reproduction Devel

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“…It is characterized by ultrastructural and spatial rearrangements of the ooplasm as well as by chemical changes of the molecules stored therein [58,59]. For this reason, in our recent studies, a set of experiments was designed to examine the effects of the exposure of bovine oocytes to a dose of Aroclor-1254 known to be detrimental to nuclear maturation and subsequent embryo development, on two important aspects of the cytoplasmic maturation of bovine oocytes: modulation of maternal mRNA polyadenylation [60] and cortical granules (CGs) migration and exocytosis [61].…”

Section: Pcbs and Cytoplasmic Maturationmentioning

confidence: 99%

“…The storage of mRNA takes place during oocyte growth and the extent of poly(A) tail at the 3 end of the transcripts has emerged as an important regulatory element for determining their stability [63] and represents a key regulatory step for early embryonic development [60,64]. Our results demonstrate that A-1254 induces changes in the polyadenylation pattern of 5 out of 10 genes examined indicating a perturbing effect exerted by this contaminant on the translational regulation of these transcripts.…”

Section: Pcbs and Cytoplasmic Maturationmentioning

confidence: 99%

“…For instance, when reduction of developmental competence is induced by removing gonadotrophins from the maturation medium, only those transcripts that would normally undergo deadenylation during the maturation process display an alteration of the poly(A) tail. Transcripts that either become adenylated or do not change during maturation are not affected by the absence of gonadotrophins [60]. On the contrary, oocyte exposure to A-1254 during IVM induces polyadenylation changes in a more varied way: as described for gonadotrophins, PCBs induced a more pronounced deadenylation of some of the genes that would deadenylate in control conditions (i.e., GT-Ty1, Cx43, Plako); however, at the same time, a longer poly(A) tail was observed at the 3 end of Cx32, a gene that normally readenylates during maturation.…”

Section: Pcbs and Cytoplasmic Maturationmentioning

confidence: 99%

See 1 more Smart Citation

Impact of endocrine disrupters on ovarian function and embryonic development

Gandolfi

Pocar²,

Brevini

et al. 2002

Domestic Animal Endocrinology

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“…Molecular maturation refers to the transcription of the mRNA blueprint (i.e. transcriptome) as well as storage of transcripts through the incorporation and extension of a 3' poly(A) tail (Brevini-Gandolfi et al, 1999). Maternal mRNAs are rapidly transcribed and stored beginning at the secondary follicle stage and throughout the period of rapid oocyte growth up to the 3 mm follicular size (Fair et al, 1995).…”

Section: Oocyte Determinants Of Fertilitymentioning

confidence: 99%

Effect of preovulatory follicle maturity on pregnancy establishment in cattle: the role ofoocyte competence and the maternal environment

Dickinson¹,

Geary²,

Monnig³

et al. 2016

Anim Reprod

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Reproductive technologies to synchronize estrus and ovulation in cattle have enhanced the ability to practically utilize artificial insemination to increase both genetic merit and reproductive management of beef and dairy herds. The ability to successfully synchronize a follicular wave and ovulation, in heifers and cows, has improved substantially in recent years. Consequently, pregnancy rates to a single fixed-time artificial insemination (FTAI) can approximate that of insemination following spontaneous estrus. Despite these advances, a subset of heifers and cows often has a physiologically immature dominant follicle at the time of GnRH-induced ovulation. These animals will exhibit reduced pregnancy rates and decreased embryonic survival if a pregnancy happens to become established. The physiological mechanisms underlying the preceding decreased fertility have been a focus of our laboratories and may include an effect of the follicular microenvironment on both oocyte competence and the maternal environment. Oocytes must have adequate opportunity to complete cytoplasmic and molecular maturation during the final stages of oocyte maturation that occur within the preovulatory follicle. Follicular status, during the proestrus period, must be such that adequate circulating concentrations of estradiol are present before FTAI to increase oviductal transport of gametes and enhance both the luteinizing capacity of granulosa cells and progesterone receptor population in the post-ovulatory uterus. Following ovulation, the follicle's transformation to a functional corpus luteum to secrete adequate amounts of progesterone is essential for the establishment of pregnancy. The physiological status of the preovulatory follicle, prior to FTAI, greatly affects the concepts discussed above and has an important impact on pregnancy establishment and maintenance in cattle.

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Changes in poly(A) tail length of maternal transcripts during in vitro maturation of bovine oocytes and their relation with developmental competence

Cited by 106 publications

References 39 publications

Retinoid‐dependent mRNA expression and poly‐(A) contents in bovine oocytes meiotically arrested and/or matured in vitro

Retinoid‐dependent mRNA expression and poly‐(A) contents in bovine oocytes meiotically arrested and/or matured in vitro

Impact of endocrine disrupters on ovarian function and embryonic development

Effect of preovulatory follicle maturity on pregnancy establishment in cattle: the role ofoocyte competence and the maternal environment

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