Changes in plasma osmolality and Na+/K+ ATPase activity of juvenile obscure puffer Takifugu obscurus following salinity challenge

Li, Jiajia; Wang, Jun; Yang, Lijun; Chen, Yafen; Yang, Zhou

doi:10.1016/j.bse.2014.05.006

Cited by 20 publications

(14 citation statements)

References 32 publications

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“…Transcriptome differences among 8 psu, 16 psu and the freshwater environment are not sufficiently stressful to activate the pathways that are involved in the stable-then-change category. These regulatory pathways can adapt to a low salinity environment when the salinity reaches a threshold that these paths cannot withstand the change and result in functional adaptation at the transcriptional level [ 73 , 74 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Profiling and Molecular Pathway Analysis of Genes in Association with Salinity Adaptation in Nile Tilapia Oreochromis niloticus

Gan

et al. 2015

PLoS ONE

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Nile tilapia Oreochromis niloticus is a freshwater fish but can tolerate a wide range of salinities. The mechanism of salinity adaptation at the molecular level was studied using RNA-Seq to explore the molecular pathways in fish exposed to 0, 8, or 16 (practical salinity unit, psu). Based on the change of gene expressions, the differential genes unions from freshwater to saline water were classified into three categories. In the constant change category (1), steroid biosynthesis, steroid hormone biosynthesis, fat digestion and absorption, complement and coagulation cascades were significantly affected by salinity indicating the pivotal roles of sterol-related pathways in response to salinity stress. In the change-then-stable category (2), ribosomes, oxidative phosphorylation, signaling pathways for peroxisome proliferator activated receptors, and fat digestion and absorption changed significantly with increasing salinity, showing sensitivity to salinity variation in the environment and a responding threshold to salinity change. In the stable-then-change category (3), protein export, protein processing in endoplasmic reticulum, tight junction, thyroid hormone synthesis, antigen processing and presentation, glycolysis/gluconeogenesis and glycosaminoglycan biosynthesis—keratan sulfate were the significantly changed pathways, suggesting that these pathways were less sensitive to salinity variation. This study reveals fundamental mechanism of the molecular response to salinity adaptation in O. niloticus, and provides a general guidance to understand saline acclimation in O. niloticus.

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Section: Discussionmentioning

confidence: 99%

Transcriptome Profiling and Molecular Pathway Analysis of Genes in Association with Salinity Adaptation in Nile Tilapia Oreochromis niloticus

Gan

et al. 2015

PLoS ONE

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“…Similarly, a considerable number of genes were identified encoding energy-dependent ATP-binding transporters (atp1a3, atp2a2, atp2a3) and voltagegated ion channels (scn1b, kcna2/3/10).. Among these genes, atp1a3 that codes Na + /K + -ATPase plays a key role in the Na + and K + gradient balance, and this gene has been reported as being differentially expressed in salinity-varying environments in T. obscurus [18]. The voltage-gated ion channel genes (scn1b and kcna family) have been discovered by differential gene expression during spawning [34] and selective sweeps adapting freshwater and alkaline waters in Amur Ide [35,36].…”

Section: Genome-wide Selective Sweep Analysismentioning

confidence: 99%

Adaptive evolution of low salinity tolerance and hypoosmotic regulation in a euryhaline teleost, Takifugu obscurus

Zhang

Hou

Liu

et al. 2019

Preprint

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Background The mechanism of osmoregulation is crucial for maintaining growth, development, and life activities in teleosts. Takifugu obscurus, the only euryhaline species in the genus Takifugu, is a proper model organism for studying the mechanism of low salt-tolerance and hypoosmotic regulation.Results In this study, whole genome sequencing data were obtained from 90 pufferfish representing five species within this genus, T. rubripes, T. obscurus, T. flavidus, T. niphobles, and T. bimaculatus. Using a phylogeny, PCA, and population structure analyses, we observed similar amounts of population genetic differentiation among species. The five species are closely related to each other and have differentiated within a relatively short period, while T. bimaculatus and T. flavidus shared the most similar genetic backgrounds. We further identified hundreds of genes under selection related to hypoosmotic regulation between T. obscurus and other Takifugu species, including 16 representative genes involving ion transporters ( atp1a3 , atp2a2 , atp2a3 , slc13a1 , slc5a8 , slc12a2 , slc12a4 , slc26a2 , scn1b , and kcna 2/3/10), genes involved in hormone regulation ( fyn , prlr , and grb2 ), and a gene associated with water absorption ( aqp3 ). Conclusions: Our findings provide preliminary insight into the mechanism of osmoregulation and will facilitate follow-up validation of candidate genes related to osmoregulation in T. obscurus .

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“…voltage-gated ion channels (scn1b, kcna2/3/10). Among these genes, atp1a3 that codes Na + /K + -ATPase plays a key role in the Na + and K + gradient balance, and this gene has been reported as being differentially expressed in salinity-varying environments in T. obscurus [18]. The voltage-gated ion channel genes (scn1b and kcna family) have been discovered by differential gene expression during spawning [34] and selective sweeps adapting freshwater and alkaline waters in Amur Ide [35,36].…”

Section: Genome-wide Selective Sweep Analysismentioning

confidence: 99%

“…Na + /K + -ATPase (NKA), as a key enzyme of ion transport maintaining the 4 Na + and K + gradient, is mainly distributed in the chloride cells of the gills and has a crucial function in the ion exchange and osmoregulation in T. obscurus [17]. The activity of NKA changes dramatically with the variation of environmental salinity [18]. In hypoosmotic environments, the intestine plays a pivotal osmoregulatory role in absorbing water and balancing ions through the intestinal epithelia [14].…”

mentioning

confidence: 99%

Adaptive evolution of low salinity tolerance and hypoosmotic regulation in a euryhaline teleost, Takifugu obscurus

Zhang

Hou

Liu

et al. 2019

Preprint

View full text Add to dashboard Cite

Background: The mechanism of osmoregulation is crucial for maintaining growth, development, and life activities in teleosts. Takifugu obscurus, the only euryhaline species in the genus Takifugu, is a proper model organism for studying the mechanism of low salt-tolerance and hypoosmotic regulation. Results: In this study, whole genome sequencing data were obtained from 90 pufferfish representing five species within this genus, T. rubripes, T. obscurus, T. flavidus, T. niphobles, and T. bimaculatus. Using a phylogeny, PCA, and population structure analyses, we observed similar amounts of population genetic differentiation among species. The five species are closely related to each other and have differentiated within a relatively short period, while T. bimaculatus and T. flavidus shared the most similar genetic backgrounds. We further identified hundreds of genes under selection related to hypoosmotic regulation between T. obscurus and other Takifugu species, including 16 representative genes involving ion transporters (atp1a3, atp2a2, atp2a3, slc13a1, slc5a8, slc12a2, slc12a4, slc26a2, scn1b, and kcna2/3/10), genes involved in hormone regulation (fyn, prlr, and grb2), and a gene associated with water absorption (aqp3). Conclusions: Our findings provide preliminary insight into the mechanism of osmoregulation and will facilitate follow-up validation of candidate genes related to osmoregulation in T. obscurus.

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Changes in plasma osmolality and Na+/K+ ATPase activity of juvenile obscure puffer Takifugu obscurus following salinity challenge

Cited by 20 publications

References 32 publications

Transcriptome Profiling and Molecular Pathway Analysis of Genes in Association with Salinity Adaptation in Nile Tilapia Oreochromis niloticus

Transcriptome Profiling and Molecular Pathway Analysis of Genes in Association with Salinity Adaptation in Nile Tilapia Oreochromis niloticus

Adaptive evolution of low salinity tolerance and hypoosmotic regulation in a euryhaline teleost, Takifugu obscurus

Adaptive evolution of low salinity tolerance and hypoosmotic regulation in a euryhaline teleost, Takifugu obscurus

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