Changes in plasma free and sulfoconjugated catecholamines before and after acute physical exercise: Experimental and clinical studies

Yoshizumi, Masanori; Nakaya, Yutaka; Hibino, Toshiyuki; Nomura, Masahiro; Minakuchi, Kazuo; Kitagawa, Tetsuya; Katoh, Itsuo; Ohuchi, Tomoyuki; Oka, M.

doi:10.1016/0024-3205(92)90079-5

Cited by 19 publications

(11 citation statements)

References 16 publications

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“…The conjugated DA at rest in our study was greater than those in previous reports probably because we deconjugated at the optimal pH of 7.6 for AS, instead of at pH 8.3. In most previous studies, CAs were measured as metabolites by radioenzymatic assay after treatment with COMT, and deconjugation was performed at pH 8.3, which is optimal for COMT, but not for AS ( 5,7,8). This may explain why the ratio of F-DA to T-DA in our study (0.25%) was lower than those (0.5% or 1%) in previous reports.…”

Section: Discussioncontrasting

confidence: 59%

“…Sixty to 70% of plasma epinephrine (E) and norepinephrine (NE) is conjugated, whereas more than 98% of the dopamine (DA) in human plasma is in the conjugated form (3,4). S-CAs, especially 5-DA, have been reported to be converted to FCAs in response to physical exercise (5,6). A recently developed automated HPLC analyzer has made it possible to quantify F-DA with great accuracy (7).…”

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confidence: 99%

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Plasma Free and Sulfoconjugated Dopamine before and after a Half-Marathon

Sakai¹,

Maeda²,

Matsumoto³

et al. 1995

Hypertens Res

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Section: Discussioncontrasting

confidence: 59%

mentioning

confidence: 99%

Plasma Free and Sulfoconjugated Dopamine before and after a Half-Marathon

Sakai¹,

Maeda²,

Matsumoto³

et al. 1995

Hypertens Res

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“…36) And dopamine sulfate is partially hydrolyzed by human arylsulfatase A, B and C in vitro, then converted to dopamine. 14) From the physiological study of free and sulfated dopamine during exercise, Yoshizumi et al found a positive relationship between free dopamine and the conjugated form in plasma, 37) which suggests that dopamine-4-Osulfate, not dopamine-3-O-sulfate, 38) acts as a reservoir of dopamine.…”

Section: Discussionmentioning

confidence: 99%

Electron Paramagnetic Resonance Study on Free Radical Scavenging and/or Generating Activity of Dopamine-4-O-sulfate.

Kirima

Tsuchiya

Yoshizumi

et al. 2001

CHEMICAL & PHARMACEUTICAL BULLETIN

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With the increasing understanding of the cytotoxicity of free radicals and other reactive oxygen species as a major cause of human disease and aging, the roles of endogenous antioxidants have drawn attentions. Uric acid, 1) vitamin E, 2) vitamin C, 3) ubiquinone, 4) SH-compounds, 5) bilirubin, 6) and pyruvate 7) are recognized as endogenous non-enzymatic antioxidants, while catalase, superoxide dismutase and glutathione peroxidase are known to be enzymatic antioxidants.Dopamine is the immediate metabolic precursor of norepinephrine and epinephrine. Dopamine interacts with dopamine receptors, then exerts its pharmacological effects.In human and experimental animals, dopamine is present almost entirely as a sulfate conjugated form in the circulation, 8,9) and very little of the free form is presented in the plasma.10) The physiological roles of sulfate conjugated dopamine are still unclear. Pharmacologically, conjugated dopamine has convulsive effects, 11) inhibits aldosterone secretion from cultured bovine adrenal cells, 12) and induces a constriction of some vascular beds. 13) One study, however, found that sulfate conjugated dopamine has no physiological activity. 14)From the viewpoint of free radical chemistry, dopamine can serve electrons via its oxidation pathway (Chart 1). This enables dopamine to act as both an antioxidant and a prooxidant. It acts as an antioxidant by scavenging free radicals. [15][16][17] Recently, it was found that dopamine spontaneously react with molecular oxygen to produce reactive oxygen species and semiquinone dopamine radical, 18,19) which are toxic to catecholamine neurons. [20][21][22][23] However, there is no evidence that sulfate conjugated dopamine acts as an antioxidant or a prooxidant. Therefore, in the present report, we compared dopamine and dopamine-4-O-sulfate for susceptibility to enzymatic and non-enzymatic oxidization in vitro using alkaline solution, horseradish peroxidase, UV irradiation, hydroxyl radical, superoxide anion radical, and sodium periodate. ExperimentalChemicals Dopamine, hypoxanthine, xanthine oxidase (from butter milk), and peroxidase (from horseradish) were purchased from Sigma Chemical Co., (MO, U.S.A.), hydrogen peroxide from Wako Pure Chemical Industries Ltd. (Osaka, Japan), and sodium metaperiodate from Acros Organics (NJ, U.S.A.). Dopamine-4-O-sulfate was kindly donated by Kyowa Hakko Kogyo Co., Ltd. (Tokyo, Japan). Other reagents were of the highest quality available from Wako Pure Chemical Industries Ltd.Free Radical Analysis Using EPR Spectroscopy The free radical metabolites of dopamine and dopamine-4-O-sulfate were examined with an EPR spectrometer (JES-TE 300, JEOL Co., Ltd., Tokyo, Japan) equipped with a cavity and an aqueous quartz flat cell (JEOL Co., Ltd., Tokyo, Japan) at X-band (9.5 GHz). EPR data were analyzed by a computer (HP Apollo 9000 Series 400) with the software, ESPRIT 432 ( JEOL Co., Ltd., Tokyo, Japan). Typical operating conditions of the EPR spectrometer were: power, 5 mW; center field, 3360 G; sweep width, 20 G; modulat...

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“…29 Detailed mechanisms of free DA increase during stress are not clear. In addition to increased DA synthesis and release, 23 a proposed alternative source of free DA, ie, free DA liberated from DA sulfate by sulfatase-mediated deconjugation 32 remains to be substantiated in vivo. The possibility that DA sulfate represents a reserve pool of free DA released by concomitant glucocorticoid increase-associated stress is supported by the findings that glucocorticoids increase free DA in man.…”

Section: Dopamine Responses To Stressmentioning

confidence: 99%

Peripheral dopamine in hypertension and associated conditions

Küchel

1999

J Hum Hypertens

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The purpose of this study is to review the role of dopamine in hypertension and associated conditions. The analysis of literature indicates that present knowledge is mostly based on poor markers and indirect evidence of dopaminergic activity and only few molecular biological data. Alternative markers such as plasma dopamine sulfate emerge as a possible substitute for the low plasma free dopamine detectability, one of the main obstacles in understanding the relationship between circulating dopamine and its receptor actions in hypertension. Essential hypertension represents a heterogeneous entity: based on evidence in borderline and non-modulating hypertension, the tubular dopamine receptor defect may be compensated by increased dopamine synthesis (dopamine ␤-hydroxylase suppression-mediated?) and release; alternatively, compatible with data in stable, salt-sensitive and low renin-

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Changes in plasma free and sulfoconjugated catecholamines before and after acute physical exercise: Experimental and clinical studies

Cited by 19 publications

References 16 publications

Plasma Free and Sulfoconjugated Dopamine before and after a Half-Marathon

Plasma Free and Sulfoconjugated Dopamine before and after a Half-Marathon

Electron Paramagnetic Resonance Study on Free Radical Scavenging and/or Generating Activity of Dopamine-4-O-sulfate.

Peripheral dopamine in hypertension and associated conditions

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