Changes in photosynthetic apparatus during dark incubation of detached leaves from control and ultraviolet-B treatedVigna seedlings

The contents of chlorophyll (Chl) and carotenoids (Car) per fresh mass were lower in shade needles than in sun needles. Ribulose-1,5-bisphosphate carboxylase (RuBPC) activity and contents of soluble proteins were also significantly lower in shade needles. In isolated thylakoids, a marked lower rate of whole chain and photosystem (PS) 2 activities were observed in shade needles. Smaller lower rate of PS1 activity was also observed in shade needles. The artificial exogenous electron donors, diphenyl carbazide (DPC) and NH 2 OH, significantly restored the loss of PS2 activity in shade needles. Similar results were obtained when F v /F m was evaluated by Chl fluorescence measurements. The marked lower rate of PS2 activity in shade needles was due to the lower contents of 47, 33, 28-25, 23, and 17 kDa polypeptides. This conclusion was confirmed by immunological studies showing that the content of the 33 kDa protein of the watersplitting complex was diminished significantly in shade needles.Additional key words: chlorophyll fluorescence; diphenyl carbazide; donor side; electron transport; NH 2 OH; photosystems 1 and 2; polypeptides; ribulose-1,5-bisphosphate carboxylase.

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Shade effect alters leaf pigments and photosynthetic responses in Norway spruce (Picea abies L.) grown under field conditions

Bertamini

Muthuchelian

Nedunchezhian

2006

Photosynt.

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Photosynthetic changes that occur during aging of cypress (Cupressus sempervirens L.) needles

Porta¹,

Bertamini²,

Nedunchezhian³

et al. 2006

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2-years-old cypress needles (A2) were physiologically most active with regard to net photosynthetic (P N ) and electron transport rates. Variable to maximum fluorescence (F v /F m ) ratios of dark-adapted needles were higher in A2 needles than in current year (A1) or senescent (A4) needles. Lower F v /F m values in these stages seemed to be caused not by photoinhibition but by a low photochemical capacity as suggested from the chlorophyll (Chl) a/b ratios. In isolated thylakoids, lower rates of whole chain and photosystem 2 (PS2) activities were observed in A4 needles, while higher rates were observed in A2 needles. A similar trend was noticed for contents of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPC) and total soluble proteins. The artificial exogenous electron donor Mn 2+ failed to restore the loss of PS2 activity in 3-year-old (A3) and A4 needles, while diphenylcarbazide and NH 2 OH significantly restored the loss of PS2 activity. The marked loss of PS2 activity in A4 needles was primarily the result of the loss of 33, 28-25, 23, and 17 kDa polypeptides. A marked loss of RuBPC activity in A4 needles is mainly due to the loss of 15 (SSU) and 55 (LSU) kDa polypeptides.Additional key words: chlorophyll fluorescence; diphenylcarbazide; donor side; electron transport; photosystems 1 and 2; polyacrylamide gel electrophoresis; polypeptides; ribulose-1,5-bisphosphate carboxylase/oxygenase.

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The Influence of Increased Solar UV-B Radiation on Magnesium-Deficient Cowpea Seedlings: Changes in the Photosynthetic Characteristics

Premkumar¹,

Kulandaivelu²

1999

Photosynt.

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Changes in photosynthetic apparatus during dark incubation of detached leaves from control and ultraviolet-B treatedVigna seedlings

Cited by 5 publications

References 28 publications

Shade effect alters leaf pigments and photosynthetic responses in Norway spruce (Picea abies L.) grown under field conditions

Shade effect alters leaf pigments and photosynthetic responses in Norway spruce (Picea abies L.) grown under field conditions

Photosynthetic changes that occur during aging of cypress (Cupressus sempervirens L.) needles

The Influence of Increased Solar UV-B Radiation on Magnesium-Deficient Cowpea Seedlings: Changes in the Photosynthetic Characteristics

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