Changes in Phospholipid Classes and Fatty Acids and Fatty Acid Desaturation and Incorporation into Phospholipids during Temperature Acclimation of Green Sunfish Lepomis cyanellus R

Lipid composition and microsomal ATPase activities in gills and kidneys of warm- and cold-acclimated sea bass (Dicentrarchus labrax L.)

“…An alternative mechanism reported by Prosser (1986) to ensure membrane homeostasis in coldacclimated teleosts in which PUFA increases were not found, such as the sunfish (Christiansen 1984), is an increase in monoene, due to an enhanced A 9 desaturation from saturated precursors. However, in SCA and LCA sea bass tissues there was no monoene increase despite an abundance of the precursor 16:0 and a paucity of 16:1 in WA fish.…”

Cold Adaptation in Ectotherms: Regulation of Membrane Function and Cellular Metabolism

“…The greater degree of thermal compensation exhibited by the terminal desaturases (i.e., delta-five and -four desaturases, those responsible for the production of long chain PUFA; Hazel 1984) of trout liver is consistent with the accumulation of long chain PUFA at cold temperature in this species. Conversely, in species such as carp and green sunfish for which monoenes playa greater role than PUFA in membrane restructuring at cold temperatures, activities of the terminal desatumses are not significantly influenced by thermal acclimation; instead, the activity of the delta-nine desaturase (responsible for the biosynthesis of monoenes) is elevated at cold growth temperatures (Schunke and Wodtke 1983;Christiansen 1984). Thus, patterns of enzymatic adaptation mirror the dichotomy in compositional adjustments previously noted.…”

“…The studies included in this survey include: Cossins (1976), total muscle PC of crayfish (4° and 25°C); Driedzic and Roots (1975), brain PC and PE of rainbow trout (5° and 19°C); Chapelle (1978), muscle and gill PC and PE of Carcinus (7°, 14°, and 27 0C); Miller et al (1976), intestinal PC and PE of goldfish (6° and 30°C); Wodtke (1978), carp liver mitochondrial PC and PE (10° and 26°C); Wodtke (1981), carp red muscle mitochondrial PC and PE (10° and 32°C); Selivonchick et al (1977), goldfish brain mitochondrial and microsomal PC and PE(5°, 15°, and 30°C); Chang and Roots (1985), goldfish brain microsomal PC and PE (5° and 25°C); Cossins (1977), goldfish and rat brain synaptosomal PC and PE (5°, 25°, and 37 0C); Cossins et al (1980), green sunfish and rat liver microsomal and mitochondrial PC and PE (5°, 25°, and 37 0C); Christiansen (1984), green sunfish liver microsomal PC and PE (15° and 25°C); Hazel (1979) rainbow trout liver PC and PE (5° and 20°C). For PC (A) the regression slopes rank mitochondrial > microsomes > synaptosomes.…”

“…The dependence of phosphatidylethanolamine abundance (A) and the ratio of phosphatidy1choline/phosphatidylethanolamine (B) upon average cell or acclimation temperature. The studies included in this figure include: Anderson (1970), goldfish brain, gill and liver mitochondria, and gill microsomes (5° and 25°C); Miller et al (1976), goldfish intestine (6° and 30°C); Caldwell and Vernberg (1970), goldfish gill mitochondria (tOO and 30°C); Wodtke (1978), carp liver mitochondria (10° and 32°C); Wodtke (1981), carp muscle mitochondria (tOO and 32°C); Chang and Roots (1985), goldfish brain microsomes (5° and 25°C); Van den Thillart and de Bruin (1981), goldfish muscle mitochondria (5° and 20°C); Christiansen (1984), green sunfish liver microsomes (5° and 25°C); Hazel (1979), trout liver (5° and 20 0C); Hazel (1985), trout gill (5 ° and 20 ° C). The regression line in A is for the mitochondrial data.…”

Effect of seasonal and experimental temperature on de novo synthesis of fatty acids in C. crangon

“…30,46) This is caused by the need to maintain a state of equilibrium in FAs composition by the action of concrete FAs desaturase and transacetylase of specific FAs to the lipid membrane. 46) In cold acclimation, growing the activity of microsomal Δ9 desaturase of SFAs, 46) whereas the incorporation of the large values of UFAs in membrane is possible by the increase of activity of acyltransferases 47) and phosphotransferases. 48) A significant part of PUFAs are included in the membrane phospholipids.…”