Changes in phenols contents from buckwheat sprouts during growth stage

Abstract

Germinated buckwheat is buckwheat seeds soaked in water just until it begins to bud. Buckwheat sprouts are seedling plants of buckwheat grown up to 10-15 cm. The purpose of this study was to determine the optimal growth period for accumulating the most abundant functional phenol(s) in germinated buckwheat that had been soaked in darkness and buckwheat sprouts cultivated by hydroponic culture. The rutin contained in germinated buckwheat was analyzed by CE (capillary electrophoresis). Phenols, including isoorientin, orientin, isovitexin, vitexin, and rutin were separated from buckwheat sprouts by HPLC and identified by LC-MS. The highest rutin content in germinated buckwheat was found to be 15.8 mg/100 g DW at 20 h after germination. Buckwheat sprouts contained five kinds of major phenols. The highest amounts of isoorientin, orientin, isovitexin, and vitexin were measured at day 3, with the exception of rutin, and then a gradual decrease was observed as the sprouts grew. The quantities of isoorientin, orientin, isovitexin, and vitexin at day 3 were 5.8, 11.7, 26.2, and 28.9 mg/100 g FW, respectively. The rutin content rapidly increased to 109.0 mg/100 g FW until day 6. The highest total phenols in buckwheat sprouts were 162.9 mg/100 g FW at day 6. Germinated buckwheat soaked for 20 h and buckwheat sprouts cultivated for 6 days were rich in dietary phenol(s), which makes these plants a valuable functional food for human consumption.

Effects of Nitrogen Fertilizer and Planting Density on the Lignin Synthesis in the Culm in Relation to Lodging Resistance of Buckwheat

“…These results suggested that the lignin content is closely related to the lodging resistance of buckwheat, and a higher lignin content could strengthen the lodging resistance of buckwheat. The increase of nitrogen fertilizer and planting density significantly increased the risk of lodging by decreasing the lignin content and its related enzymes activities at the bottom of the 2nd internode.Key words: Buckwheat, Lignin, Lodging, Nitrogen fertilizer, Planting density.Buckwheat (Fagopyrum esculentum), which belongs to the family Polygonaceae, genus Fagopyrum, is commonlyconsumed in arid and cold regions in the world (Koyama et al, 2013). Buckwheat species native to China have been systematically classified since 1913 and it has been praised as one of the most faddish green function in the 21st century (Guo et al, 2007).…”

“…Buckwheat (Fagopyrum esculentum), which belongs to the family Polygonaceae, genus Fagopyrum, is commonlyconsumed in arid and cold regions in the world (Koyama et al, 2013). Buckwheat species native to China have been systematically classified since 1913 and it has been praised as one of the most faddish green function in the 21st century (Guo et al, 2007).…”

Characterization of bioactive agents in five types of marketed sprouts and comparison of their antihypertensive, antihyperlipidemic, and antidiabetic effects in fructose-loaded SHRs

“…Buckwheat seeds constitute a rich source of rutin in cereals. We previously reported that rutin is more abundant in buckwheat sprouts than in seeds (Koyama et al 2011). Moreover, rutin has antihypertensive (Ajay et al 2003) and antihyperlipidemic effects (Lee et al 2010).…”

Optimization of Conditions for Organic Acid Extraction from Edible Plant Material as Applied to Radish Sprouts

“…It is essential to determine the concentrations of organic acids in edible sprouts in order to be able to classify them as a functional food. For example, sprouted buckwheat grains may constitute a valuable component of any healthy diet (Koyama et al 2013;Kim et al 2004).…”