Changes in Oral Microbial Ecology of C57BL/6 Mice at Different Ages Associated with Sampling Methodology

Hernández-Arriaga, Angélica; Baumann, Anja; Witte, Otto W.; Frahm, Christiane; Bergheim, Ina; Camarinha‐Silva, Amélia

doi:10.3390/microorganisms7090283

Cited by 19 publications

(17 citation statements)

References 32 publications

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“…A recently proposed species of the Streptococcus genus, S. danieliae MOT 10 was originally described based on a murine cecal isolate for which the authors suggested an oral/upper respiratory tract origin (25). There have been few other reports of the organism so far (26)(27)(28), all of them of murine origin. The organism has also been reported to be one of the key drivers in the establishment of the oral microbial community in laboratory SPF mice after the eruption of teeth (8).…”

Section: Discussionmentioning

confidence: 99%

A 16S rRNA Gene and Draft Genome Database for the Murine Oral Bacterial Community

et al. 2021

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A curated murine oral microbiome database to be used as a reference for mouse-based studies has been constructed using a combination of bacterial culture, 16S rRNA gene amplicon, and whole-genome sequencing. The database comprises a collection of nearly full-length 16S rRNA gene sequences from cultured isolates and draft genomes from representative taxa collected from a range of sources, including specific-pathogen-free laboratory mice, wild Mus musculus domesticus mice, and formerly wild wood mouse Apodemus sylvaticus. At present, it comprises 103 mouse oral taxa (MOT) spanning four phyla—Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetes—including 12 novel undescribed species-level taxa. The key observations from this study are (i) the low diversity and predominantly culturable nature of the laboratory mouse oral microbiome and (ii) the identification of three major murine-specific oral bacterial lineages, namely, Streptococcus danieliae (MOT10), Lactobacillus murinus (MOT93), and Gemella species 2 (MOT43), which is one of the novel, still-unnamed taxa. Of these, S. danieliae is of particular interest, since it is a major component of the oral microbiome from all strains of healthy and periodontally diseased laboratory mice, as well as being present in wild mice. It is expected that this well-characterized database should be a useful resource for in vitro experimentation and mouse model studies in the field of oral microbiology. IMPORTANCE Mouse model studies are frequently used in oral microbiome research, particularly to investigate diseases such as periodontitis and caries, as well as other related systemic diseases. We have reported here the details of the development of a curated reference database to characterize the oral microbial community in laboratory and some wild mice. The genomic information and findings reported here can help improve the outcomes and accuracy of host-microbe experimental studies that use murine models to understand health and disease. Work is also under way to make the reference data sets publicly available on a web server to enable easy access and downloading for researchers across the world.

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Section: Discussionmentioning

confidence: 99%

A 16S rRNA Gene and Draft Genome Database for the Murine Oral Bacterial Community

et al. 2021

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“…Library preparation was performed according to the Illumina protocol described by [16]. Briefly, primers 27F (slight modification) and 338R reported by [17,18] were used to target the V1-2 region of the 16S rRNA gene.…”

Section: Sample Collection Dna Extraction and Illumina Library Preparationmentioning

confidence: 99%

“…Raw sequence reads obtained from Illumina MiSeq system (Illumina, Inc., San Diego, CA, USA) were analyzed using QIIME v1.9.1 pipeline (http://qiime.org/) [19], following a subsampled open-reference OTUs (operational taxonomic units) calling approach [20]. Demultiplexing and trimming of sequencing reads were done using the default parameters of the pipeline [16], with a maximum sequence length of 360 bp. The reads were merged into one fasta file and aligned using the SILVA Database (Release 132) (https://www.arb-silva.de/) [21].…”

Section: Bioinformatics and Stratistical Analysismentioning

confidence: 99%

Effects on the Ileal Microbiota of Phosphorus and Calcium Utilization, Bird Performance, and Gender in Japanese Quail

Borda-Molina

Roth

Hernández-Arriaga

et al. 2020

Animals

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In this study, we aimed to investigate the ileum digesta of a large cohort of Japanese quail fed the same diet, with similar environmental conditions. We also address how P utilization (PU), Ca utilization (CaU), and bird performance (feed intake (FI), feed conversion (FC), and body weight gain (BWG)) modify intestinal microbiota of male and female quail. Despite the great number of samples analyzed (760), a core microbiome was composed of five bacteria. The Unc. Lactobacillus, Unc. Clostridaceae 1, Clostridium sensu stricto, Escherichia coli, and Streptococcus alactolyticus were detected in all samples and contributed to more than 70% of the total community. Depending on the bird predisposition for PU, CaU, FI, BWG, and FC, those species were present in higher or lower abundances. There was a significant gender effect on the ileal microbial community. While females had higher abundances of Lactobacillus, males were more colonized by Streptococcus alactolyticus. The entire cohort was highly colonized by Escherichia coli (8%–15%), an enteropathogenic bacteria. It remains unclear, if microbiota composition followed the mechanisms that caused different PU, CaU, FI, FC, and BWG or if the change in microbiota composition and function caused the differences in PU, CaU, and performance traits.

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“…The abundance of the bacterial phyla assessed in this report have been shown make-up greater than 90% of the murine oral bacteriome. 38 The authors acknowledge the possibility that stability or changes of lower abundance bacterial phyla may play a role in antimicrobial-induced alveolar bone loss. This highlights the need for future microbiome investigations that comprehensively investigate antimicrobial induced changes across bacterial phyla and lower-level taxa communities.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial‐induced oral dysbiosis exacerbates naturally occurring alveolar bone loss

et al. 2021

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Periodontitis-mediated alveolar bone loss is caused by dysbiotic shifts in the commensal oral microbiota that upregulate proinflammatory osteoimmune responses.The study purpose was to determine whether antimicrobial-induced disruption of the commensal microbiota has deleterious effects on alveolar bone. We administered an antibiotic cocktail, minocycline, or vehicle-control to sex-matched C57BL/6T mice from age 6-to 12 weeks. Antibiotic cocktail and minocycline had catabolic effects on alveolar bone in specific-pathogen-free (SPF) mice. We then administered minocycline or vehicle-control to male mice reared under SPF and germ-free conditions, and we subjected minocycline-treated SPF mice to chlorhexidine oral antiseptic rinses. Alveolar bone loss was greater in vehicle-treated SPF versus germ-free mice, demonstrating that the commensal microbiota drives naturally occurring alveolar bone loss. Minocycline-versus vehicle-treated germfree mice had similar alveolar bone loss outcomes, implying that antimicrobialdriven alveolar bone loss is microbiota dependent. Minocycline induced phylum-level shifts in the oral bacteriome and exacerbated naturally occurring

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Changes in Oral Microbial Ecology of C57BL/6 Mice at Different Ages Associated with Sampling Methodology

Cited by 19 publications

References 32 publications

A 16S rRNA Gene and Draft Genome Database for the Murine Oral Bacterial Community

A 16S rRNA Gene and Draft Genome Database for the Murine Oral Bacterial Community

Effects on the Ileal Microbiota of Phosphorus and Calcium Utilization, Bird Performance, and Gender in Japanese Quail

Antimicrobial‐induced oral dysbiosis exacerbates naturally occurring alveolar bone loss

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