Aim: To evaluate the level of knowledge of the students of the Higher Education in the health area of an IES (Higher Education Institution) of a city in the interior of the State of Goiás, about penile cancer and to identify the strategies adopted by the population to prevent carcinoma penile. Method: This is a field-based, evaluative, exploratory, descriptive, cross-sectional, quantitative approach. Results: we found the predominance of students with high level of knowledge (72.5%) on penile cancer, followed by those with a medium level of knowledge (26.1) on the subject. The most frequent attitudes for penile cancer prevention were: cleaning the penis daily with soap and water, using condoms in sexual relations; performing penis hygiene after sexual intercourse and masturbation; trying to have good hygiene habits, taking medical consultation when they felt something and inspect the penile in the shower. Conclusion: we found a high level of knowledge regarding the penile cancer prevention. Early detection of condom use, daily hygiene and non-promiscuity of sexual partners are relevant points in the prevention and detection of the disease.