Changes in myosin heavy chain stoichiometry in pig tracheal smooth muscle during development

Mohammad, Mukhallad A.; Sparrow, Malcolm

doi:10.1016/0014-5793(88)80596-9

Cited by 46 publications

(18 citation statements)

References 14 publications

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“…SM 2 accounted for the majority, if not all, of the 200-kD isoform and was present throughout development, expression increasing~2-fold, whereas SM 1 decreased. Similar alterations have been seen in tracheal smooth muscle from neonatal and large adult pigs (30). In contrast, at~120-d gestation the ovine fetal aorta expressed smaller amounts of SM 1 than the 200-kD MHC isoform, which appears to be predominantly MHC-B.…”

Section: Discussionsupporting

confidence: 66%

“…Although nonmuscle MHC isoforms have been observed in aortic smooth muscle from adult animals and humans (9,12,14), they are not present in adult bovine (15) or rabbit (16) aorta or adult ovine uterine artery, myometrium or bladder (22), which may reflect species differences. During development SM 1 is expressed to varying but significant extent in several smooth muscle tissues from the fetus and neonate (3,5,9,12,(14)(15)(16)30), whereas the composition of the remaining MHC is variable. However, it has become more evident that the predominant lower molecular weight species expressed in fetal and neonatal aorta are the nonmuscle MHC isoforms, which only can be identified using Western analysis because of their similar electrophoretic mobilities (9,12,15,16).…”

Section: Discussionmentioning

confidence: 99%

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Smooth Muscle Myosin Heavy Chain Isoforms Are Developmentally Regulated in Male Fetal and Neonatal Sheep

1995

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Section: Discussionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Smooth Muscle Myosin Heavy Chain Isoforms Are Developmentally Regulated in Male Fetal and Neonatal Sheep

1995

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“…In the rodent ventricle, the slow migrating MHC isoform is predominant in fetal life, whereas the faster migrating MHC is more prevalent during adult life (21). In the pig tracheal smooth muscle, there is a decrease in the ratio of MHCl to MHC2 during maturation (24), whereas similar work on human tracheal smooth muscle failed to show any change in MHC isoform with maturation (25).…”

Section: Newborn Fundusmentioning

confidence: 80%

Characterization of Actin and Myosin in the Developing Stomach

1995

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During growth and development, dietary intake changes from being predominantly liquid in the newborn period to mixed solid liquid meals. These alterations in diet vary the functional demands placed on the stomach. It has been shown that, during development, smooth muscle of the stomach undergoes changes in the mechanism responsible for the contractile process. In this study, we have investigated the possibility that there are structural changes in two of the major proteins that are responsible for generation of force during smooth muscle contraction: actin and myosin. Actin and myosin were identified in newborn kittens (1 wk old) and adult gastric smooth muscle using one-dimensional SDS-PAGE. Although both the antrum and fundus of the kitten have significantly smaller total amounts of actin and myosin per mg protein than the adult, the ratio of actin to myosin is not significantly different between the age groups. Two different The changes in diet ary constitu ents during growth and development from predominantl y liquid to mixed solid liquid meals places an increasing burd en on the stomach that is required to triturate these solids and empty them from the stoma ch. The fundus or proxim al portion of the stomach is respon sibl e for the storage and empty ing of gastric food stuffs, whereas the antrum redu ces the size of ing ested solids and faci litates emptying (1). Th ere is a growing bod y of experiment al ev ide nce suggesting that functional characteristics of smoo th muscle in the sto mac h und ergo changes that parallel these alte rations in dietary mili eu. Th e frequ enc y and amplitude of contractions in the ca nine stoma ch increase during the newborn period (2). Th e in vitro force-generating capacit y of smoo th mu scle from the stomach of newborn rabbit s is less than that of adult gastri c muscl e, and this reduction in forcegeneratin g capacity did not appear to be accounted for by changes in receptor function (3,4). In addition to these quantitative differences in ability to ge nerate force, we have recentl y repo rted that isolated muscle ce lls from the antrum of the adult cat use intr acellular Ca 2 + stores that are not available for kitten antral cells (5). We observed an increase in the amount of MHC2 in the adult, which resulted in a decreased ratio of MHC1 to MHC2 in the adult. We postulate that the decreased quantity of actin and myosin in the kitten stomach and the observed changes in the ratio of the MHC isoforms are related to changes in the gastric motor that occur during growth and development. (Pediatr Res 37: 202-206, 1995) Abbreviations PMSF , phenylmethyl sulfanyl fluoride f3ME, f3-mercaptoethanol MHC , myosin heavy chain Actin and myo sin are two of the major structural proteins involved in the contractil e machinery of the cell. Th e generation of tension during muscle contraction is the result of the interaction between myos in heads and adjacent actin filaments (6). Previous studies have suggested that changes in the absolute amount and relative abundance of isoforms ...

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“…In studies using swine trachealis muscle, myosin content either increased from fetus to adult (23) or decreased from 2 to 10 wk postnatal age (24). Investigators have shown differences in myosin isoforms between embryonic, neonatal, and adult smooth muscle in both airway (23)(24)(25) and nonairway smooth muscle (26,27). Although any or all of these mechanisms may be responsible for the observed increase in force generation with age, the present study did not attempt to identify the causes of increased contractility.…”

Section: Newbornmentioning

confidence: 99%