Changes in Midgut Endopeptidase Activity of &lt;I&gt;Spodoptera frugiperda&lt;/I&gt; (Lepidoptera: Noctuidae) are Responsible for Adaptation to Soybean Proteinase Inhibitors

Paulillo, Luis Cesar Maffei Sartini; Lopes, Adriana Rios; Cristofoletti, Plı́nio T.; Parra, José Roberto Postali; Terra, Walter R.; Silva-Filho, Marcio C.

doi:10.1603/0022-0493-93.3.892

Cited by 78 publications

(60 citation statements)

References 26 publications

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“…In the present work, the relative metabolic rate and the metabolic costs were increased by the addition of the extracts to the diet, which could be explained by the presence of a trypsin inhibitor, as S. frugiperda larvae has been shown to overproduce digestive enzymes when feeding on substrates containing such molecules (Paulillo et al 2000), which leads to an increase in energy expenditure due to a higher metabolic rate. Conversion of consumed food into biomass was also affected by the extracts as indicated by the lower ECI and ECD, while displaying higher RCR values, indicating that the larvae feeding on castor bean extracts required a much higher amount of food to gain the same weight if compared with control larvae.…”

Section: Discussionmentioning

confidence: 55%

Biochemical Analysis of a Castor Bean Leaf Extract and its Insecticidal Effects Against Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae)

Rossi¹,

Santos

Carvalho

et al. 2012

Neotrop Entomol

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Section: Discussionmentioning

confidence: 55%

Biochemical Analysis of a Castor Bean Leaf Extract and its Insecticidal Effects Against Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae)

Rossi¹,

Santos

Carvalho

et al. 2012

Neotrop Entomol

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“…This observation has allowed us to obtain transgenic plants with increased resistance to the sugarcane borer (manuscript in preparation). However, insects have evolved different strategies to overcome the adverse effects of host proteinase inhibitors (Paulillo et al, 2000, Brito et al, 2001. Our results indicate that bioassays employing proteinase-inhibitor-supplemented diets might be useful in the selection of effective inhibitors for a partic-ular insect, but validation of feeding trials in transformed plants are also needed.…”

Section: Proteinase Inhibitorsmentioning

confidence: 99%

Mechanisms of sugarcane response to herbivory

et al. 2001

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Deciphering plant-insect interactions at the molecular level is one of the major topics of interest in contemporary plant biology research. In the last few years, various aspects of the plant response to insect damage have been investigated, including the characterization of direct and indirect responses, the regulation of gene expression resulting from insect attack and the signal transduction pathways. Such research has resulted in the proposal of new methods to enhance host resistance to insect pests, including the use of insecticidal genes that can be transferred by genetic engineering into target crops. By integrating the understanding of how plants react to insect damage with the techniques of molecular biology researchers should be able to increase the wide range of methods available for the control of insect pests. The sugarcane transcriptome project (SUCEST) has allowed the identification of several orthologues genes involved in the plant response to insect damage. In this paper we summarize several aspects of the complex interaction between plants and insects and describe the use of in silico analysis to provide information about gene expression in different sugarcane tissues in response to insect attack.

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“…Faced with the prospect and consequences of reduced nutrient uptake, the larva responds by quantitatively and/or qualitatively . altering the gut proteases to compensate the loss of protease activity due to PI binding [6,[74][75][76]. Quantitative changes include generalized or specific increases in the levels of gut proteases to attain optimal rate of protein digestion [2,57,77,78].…”

Section: Evolution and Adaptation: Lessons From The Past But Can We mentioning

confidence: 99%

Structural and functional diversities in lepidopteran serine proteases

Srinivasan

Giri

Gupta

2006

Cellular and Molecular Biology Letters

211

164

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Primary protein-digestion in Lepidopteran larvae relies on serine proteases like trypsin and chymotrypsin. Efforts toward the classification and characterization of digestive proteases have unraveled a considerable diversity in the specificity and mechanistic classes of gut proteases. Though the evolutionary significance of mutations that lead to structural diversity in serine proteases has been well characterized, detailing the resultant functional diversity has continually posed a challenge to researchers. Functional diversity can be correlated to the adaptation of insects to various host-plants as well as to exposure of insects to naturally occurring antagonistic biomolecules such as plant-derived protease inhibitors (PIs) and lectins. Current research is focused on deciphering the changes in protease specificities and activities arising from altered amino acids at the active site, specificity-determining pockets and other regions, which influence activity. Some insight has been gained through in silico modeling and simulation experiments, aided by the limited availability of characterized proteases. We examine the structurally and functionally diverse Lepidopteran serine proteases, and assess their influence on larval digestive processes and on overall insect physiology.

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Changes in Midgut Endopeptidase Activity of <I>Spodoptera frugiperda</I> (Lepidoptera: Noctuidae) are Responsible for Adaptation to Soybean Proteinase Inhibitors

Cited by 78 publications

References 26 publications

Biochemical Analysis of a Castor Bean Leaf Extract and its Insecticidal Effects Against Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae)

Biochemical Analysis of a Castor Bean Leaf Extract and its Insecticidal Effects Against Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae)

Mechanisms of sugarcane response to herbivory

Structural and functional diversities in lepidopteran serine proteases

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