Changes in microbial community during hydrogen and methane production in two-stage thermophilic anaerobic co-digestion process from biowaste

Zahedi, S.; Solera, Rosario; Mıcoluccı, Federico; Cavınato, Cristina; Bolzonella, David

doi:10.1016/j.wasman.2016.01.016

Cited by 104 publications

(30 citation statements)

References 37 publications

(75 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A pH range of <6.5 or >8 has been reported to cause the total failure of the anaerobiosis/methanogenic stage in anaerobic digestion process (Ennouri et al, 2016). Therefore, maintaining appropriate pH status in anaerobic digesters is a major factor in ensuring adequate microbial and/or enzymatic substrate degradation (Dai et al, 2016;Zahedi et al, 2016). Maintenance of suitable temperature in Table 3 Test of significance and Analysis of variance (ANOVA) for all regression coefficient terms for biogas generation from Carica papayas fruit peels (I ¼ Temperature ( C); J ¼ pH; K ¼ Retention time (Day); L ¼ Total solids (g/kg): M ¼ Volatile solids (g/kg); df ¼ degree of freedom; SS ¼ Sum of square; MS ¼ Mean square).…”

Section: Discussionmentioning

confidence: 99%

Cleaner energy for cleaner production: Modeling and optimization of biogas generation from Carica papayas (Pawpaw) fruit peels

Dahunsi

Oranusi

Efeovbokhan

2017

Journal of Cleaner Production

View full text Add to dashboard Cite

In this study, the potentials of pretreated and untreated Carica papayas fruit peels for biogas generation was evaluated alongside its process optimization after employing the combination of mechanical and thermo-alkaline pretreatment methods. The peel was anaerobically digested using a consortium of microorganisms from cattle rumen content as inoculum. A batch system designed by the Central Composite Design (CCD) was employed. The physicochemical and microbial characteristics of the substrates and inoculum as well as the constituent of the generated biogas were evaluated using standard methods and results showed elevated levels of most elements after anaerobic digestion. The most feasible experimental biogas yields were 0.1839 m 3 /kg VS and 0.1361 m 3 /kg VS for the pretreated and untreated experiments respectively. Microbiologically, members of the genus Clostridium dominated the microbial flora and their succession patterns were affected by temperature and pH. The methane and carbon dioxide content of biogas from both experiments were 61.5± 2.5%; 24± 1% and 52 ± 2%; 25± 1.5% respectively. The Response Surface Methodology (RSM) and the Artificial Neural Networks (ANNs) were employed in data optimization. Based on the optimized values, the predicted biogas yield for RSM was 0.1895 m 3 /kg VS and 0.1839 m 3 /kg VS for ANNs in the pretreated experiment. For the experiment without pretreatment, the RSM predicted yield was 0.1361 m 3 /kg VS while that of ANNs was 0.1392 m 3 / kg VS. In all, there was a 26.5% increase in predicted biogas yield in the pretreated experiment over the untreated. Further usage of pawpaw peels for biofuels generation is advocated.

show abstract

Section: Discussionmentioning

confidence: 99%

Cleaner energy for cleaner production: Modeling and optimization of biogas generation from Carica papayas (Pawpaw) fruit peels

Dahunsi

Oranusi

Efeovbokhan

2017

Journal of Cleaner Production

View full text Add to dashboard Cite

show abstract

“…The pH level reached a constant value of 7.8 ± 0.4 in all BMP tests. This has been identified as the optimal pH for enhanced acetogenic and methanogenic activity (De la Rubia et al, 2009;Zahedi et al, 2014bZahedi et al, , 2016. At the end of the assay, VFA concentrations were lower than 0.2 g/g VS and no propionic acid was detected under any pre-treatment tested.…”

Section: Effect Of Fna Pre-treatment On the Final Characteristics Of mentioning

confidence: 93%

Assessment of free nitrous acid pre-treatment on a mixture of primary sludge and waste activated sludge: Effect of exposure time and concentration

Zahedi¹,

Icaran

Yuan

et al. 2016

Bioresource Technology

Self Cite

View full text Add to dashboard Cite

“…There have been quite a few culture-independent techniques employed for the investigation of the methanogenic consortia like: a) Fluorescence In situ Hybridization (FISH) [11-13] b) Terminal Restriction Fragment Length Polymorphism (T-RFLP) [14] c) community fingerprinting by Denaturing Gradient Gel Electrophoresis (DGGE) [15] d) real-time quantitative PCR (qPCR) [16-18] e) Single Strand Conformation Polymorphism (SSCP) [19]. As suggested in the past studies conducted in several countries, anaerobic sludge digester (ASD) treatment systems in the UAE are susceptible to several operational problems, potentially reducing their overall efficiency.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, more detailed and fundamental understanding of microbial populations is required for effective long-term control of ASD conditions. Molecular techniques have shown promise for gaining a better understanding of microbial community members in ASD treatment systems and quantitative data provided by molecular techniques such, as FISH [11-13] and Q-PCR [16-18] have been successfully utilized in several studies to validate engineered models and to optimize biogas production and for this reason these two molecular approaches have been used in the current study.…”

Section: Introductionmentioning

confidence: 99%

Assessing Methanogenic Archaeal Community in Full Scale Anaerobic Sludge Digester Systems in Dubai, United Arab Emirates

et al. 2018

View full text Add to dashboard Cite

Introduction:Anaerobic digestion for methane production comprises of an exceptionally diverse microbial consortium, a profound understanding about which is still constrained. In this study, the methanogenic archaeal communities in three full-scale anaerobic digesters of a Municipal Wastewater Treatment Plant were analyzed by Fluorescence in situ hybridization and quantitative real-time Polymerase Chain Reaction (qPCR) technique.Methods & Materials:Fluorescence in situ hybridization (FISH) was performed to detect and quantify the methanogenic Archaea in the sludge samples whereas qPCR was carried out to support the FISH analysis. Multiple probes targeting domain archaea, different orders and families of Archaea were used for the studies.Results and Discussion:In general, the aceticlastic organisms (Methanosarcinaceae & Methanosaetaceae) were more abundant than the hydrogenotrophic organisms (Methanobacteriales, Methanomicrobiales, Methanobacteriaceae & Methanococcales). Both FISH and qPCR indicated that family Methanosaetaceae was the most abundant suggesting that aceticlastic methanogenesis is probably the dominant methane production pathway in these digesters.Conclusion:Future work involving high-throughput sequencing methods and correlating archaeal communities with the main operational parameters of anaerobic digesters will help to obtain a better understanding of the dynamics of the methanogenic archaeal community in wastewater treatment plants in United Arab Emirates (UAE) which in turn would lead to improved performance of anaerobic sludge digesters.

show abstract

Changes in microbial community during hydrogen and methane production in two-stage thermophilic anaerobic co-digestion process from biowaste

Cited by 104 publications

References 37 publications

Cleaner energy for cleaner production: Modeling and optimization of biogas generation from Carica papayas (Pawpaw) fruit peels

Cleaner energy for cleaner production: Modeling and optimization of biogas generation from Carica papayas (Pawpaw) fruit peels

Assessment of free nitrous acid pre-treatment on a mixture of primary sludge and waste activated sludge: Effect of exposure time and concentration

Assessing Methanogenic Archaeal Community in Full Scale Anaerobic Sludge Digester Systems in Dubai, United Arab Emirates

Contact Info

Product

Resources

About