Changes in metabolome and in enzyme activities during germination of<i>Trichoderma atroviride</i>conidia

Kaliňák, Michal; Šimkovič, Martin; Žemla, Peter; Mat'at'a, Matej; Molnár, Tomáš; Liptaj, Tibor; Varečka, Ĺudovı́t; Hudecová, Daniela

doi:10.1111/1574-6968.12516

Cited by 4 publications

(5 citation statements)

References 29 publications

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“…Qualitative analysis of main constituents of ginger extract was measured using nuclear magnetic resonance (NMR) according to Kaliňák et al (2014). Briefly, 1 H-NMR spectra were run on a 600 MHz NMR spectrometer (Agilent) equipped with indirect detection probe.…”

Section: Determinations Of Main Constituents Of Ginger Extract Using mentioning

confidence: 99%

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Antioxidant activity of ginger extract and identification of its active components

Mošovská¹,

Nováková²,

Kaliňák

2015

Acta Chimica Slovaca

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The object of presented study was the evaluation of antioxidant activity of extract from Zingiber officinale. A method for the preparation of the extract was chosen, which enabled to isolate mostly phenolic compounds. According to the measurements with Folin-Ciocalteu reagent, obtained extract comprised total polyphenols (181.41 mg GAE /g of extract) from which flavonoids contributed to 7.8 % (14.15 mg quercetin /g of extract). Nuclear magnetic resonance (NMR) analysis showed that the phenolic ketones were predominant in the extracts of whole phenolic compounds. According to results of two spectrophotometric methods (ABTS and DPPH test), ginger extract showed stronger ability to scavenge DPPH radical than ABTS cation radical.

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Section: Determinations Of Main Constituents Of Ginger Extract Using mentioning

confidence: 99%

“…Determinations of main constituents of studied extract were done by NMR analysis according to Kaliňák et al (2014).…”

Section: Determinations Of Main Constituents Of Ginger Extract Using mentioning

confidence: 99%

Antioxidant activity of ginger extract and identification of its active components

Mošovská¹,

Nováková²,

Kaliňák

2015

Acta Chimica Slovaca

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“…Analyses of turmeric sample using NMR spectroscopy Determination of main constituent of the studied extract was done by NMR analysis according to Kaliňák et al (2014) 1 H-NMR standard spectra were measured and compared with the reference spectra published by Payton et al (2007) that confirmed the presence of curcumin (Fig. 1) in the turmeric extract (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Qualitative analysis of curcuminoids presented in obtained turmeric extract was measured by nuclear magnetic resonance (NMR) according to Kaliňák et al (2014). Briefly, 1 H-NMR spectra were run on a 600 MHz NMR spectrometer (Agilent) equipped with indirect detection probe.…”

Section: Analyses Of Turmeric Sample Using Nmr Spectroscopymentioning

confidence: 99%

Antioxidant properties of curcuminoids isolated from Curcuma longa L.

Mošovská¹,

Petáková²,

Kaliňák

et al. 2016

Acta Chimica Slovaca

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The evaluation of antioxidant potential of food has received much attention in recent years. Antioxidant compounds can scavenge free radicals and thereby can protect the human body from free radicals. This study was focused on the isolation of curcuminoids from the dried turmeric rhizome, and studying their antioxidant activity. The presence of curcuminoids was identified in turmeric sample by Nuclear Magnetic Resonance (NMR) analysis. Since neutral curcumin is known to be poorly soluble, the synthesis of curcumincyclodextrin and curcumin-phospholipid complexes was also performed. The antioxidant activity of isolated curcuminoids was assessed by two methods (ABTS and FRAP assay) and their scavenging activities were compared with those of prepared complexes. The ability to reduce ABTS radical cation decreased as follows: quercetin > trolox > curcuminoids > curcumin-cyclodextrin complex > curcumin-phospholipid complex. The reducing potential of tested samples in descending order was quercetin > trolox > curcumin-cyclodextrin complex > curcuminoids > curcumin-phospholipid complex.

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“…We added the extracts (0.1% and 0.01%) to a suspension of 1 × 10 5 conidia/mL of B. cinerea incubated for 2 h in Sabouraud broth. The suspension was re-incubated for 12 h, and at the end of the period, the lengths of the germ tubes were estimated by digital image analysis (40×) [37]. Treatments were evaluated in triplicate, for a total of 20 units per repetition (N = 60).…”

Section: Effect On Germ Tube Developmentmentioning

confidence: 99%

The Search for Quorum Sensing in Botrytis cinerea: Regulatory Activity of Its Extracts on Its Development

Rosero-Hernández

Echeverri

2020

Plants

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Botrytis cinerea is a phytopathogenic fungus that causes large crop and post-harvest losses. Therefore, new and effective strategies are needed to control the disease and to reduce resistance to fungicides. Modulating pathogenicity and virulence by manipulating microbial communication is a promising strategy. This communication mechanism, called Quorum Sensing (QS), has already been reported in bacteria and yeasts; however, it has not yet been studied in B. cinerea. To establish the existence of this biochemical process in B. cinerea, we prepared extracts at different growth times (D1-D12), which were applied to fresh cultures of the same fungi. The chemical analysis of the extracts obtained from several fermentations showed different compositions and biological activities. We confirmed the presence of several phytotoxins, as well as compounds 1-phenylethanol and 3-phenylpropanol. Day five extract (0.1%) inhibited conidia germination and elongation of germ tubes, day seven extract (1%) produced the greatest phytotoxic effect in tomato leaves, and day nine extract (0.1%) was a sporulation inhibitor. In contrast, the extracts from days 7, 9, and 12 of fermentation (0.1% and 0.01%) promoted pellet and biofilm formation. Sporulation was slightly induced at 0.01%, while at 0.1% there was a great inhibition. At the highest extract concentrations, a biocidal effect was detected, but at the lowest, we observed a QS-like effect, regulating processes such as filamentation, morphogenesis, and pathogenesis. These results of the biological activity and composition of extracts suggest the existence of a QS-like mechanism in B. cinerea, which could lead to new non-biocidal alternatives for its control through interference in the pathogenicity and virulence mechanisms of the fungi.

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Changes in metabolome and in enzyme activities during germination ofTrichoderma atrovirideconidia

Cited by 4 publications

References 29 publications

Antioxidant activity of ginger extract and identification of its active components

Antioxidant activity of ginger extract and identification of its active components

Antioxidant properties of curcuminoids isolated from Curcuma longa L.

The Search for Quorum Sensing in Botrytis cinerea: Regulatory Activity of Its Extracts on Its Development

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