Changes in malondialdehyde content and in superoxide dismutase, catalase and glutathione reductase activities in sunflower seeds as related to deterioration during accelerated aging

Bailly, Christophe; Benamar, Abdelilah; Corbineau, Françoise; Côme, D.

doi:10.1111/j.1399-3054.1996.tb00485.x

Cited by 438 publications

(262 citation statements)

References 35 publications

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“…Seeds were subsequently rinsed with distilled water and then dried at room temperature for 3 days on filter paper. Accelerated ageing was accomplished by storing seeds in plastic boxes containing water (presumably 100% RH) for 5 days at 45°C (Bailly et al 1996). In such ageing conditions, the seed moisture content was 30% dry weight (DW), which corresponded to a water potential of À5 MPa (data not shown).…”

Section: Seed Treatmentsmentioning

confidence: 99%

Organization of lipid reserves in cotyledons of primed and aged sunflower seeds

Walters¹,

Landré

Hill³

et al. 2005

Planta

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Imbibing sunflower (Helianthus annuus L., cv. Briosol) seeds at water potentials between -2 MPa and -5 MPa leads to faster (priming) or slower (accelerated ageing) germination depending on the temperature and duration of treatment. Mobilization of food reserves may be associated with the changes in seed vigor. To study this, morphological, biochemical and phase properties of lipid, the major food reserve in sunflower, were compared in freshly harvested (i.e., control), primed and aged sunflower cotyledons using electron microscopy, biochemical analyses and differential scanning calorimetry, respectively. Lipid bodies became smaller and more dispersed throughout the cytoplasm during priming and ageing. Despite ultrastructural changes, there were few measured changes in biochemistry of the neutral lipid component; lipid content, proportion of saturated and unsaturated fatty acids and level of free fatty acids were unchanged in primed and slightly aged seeds, with only severely aged seeds showing a net decrease in polyunsaturated fatty acids and an increase in free fatty acids. Subtle changes in the calorimetric behavior of lipids within sunflower cotyledons were observed. Sunflower lipids exhibited polymorphic crystalline and amorphous solid phases when cooled to <-100 degrees C, but priming decreased the rate of crystallization in vivo and ageing increased the rate of crystallization, but decreased percentage crystallinity. The observed changes in thermal behavior in vivo are consistent with losses and gains, respectively, of interacting non-lipid moieties in the triacylglycerol matrix.

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Section: Seed Treatmentsmentioning

confidence: 99%

Organization of lipid reserves in cotyledons of primed and aged sunflower seeds

Walters¹,

Landré

Hill³

et al. 2005

Planta

Self Cite

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show abstract

“…12 The homogenate was filtered through two layers of miracloth and centrifuged at 20 000 × g for 15 min, and a 2.5 ml aliquot was loaded into a Sephadex G-25 column (PD 10, Pharmacia, Madrid, Spain) equilibrated with 10 ml 0.1 M phosphate buffer (pH 7.8). The enzymes were eluted with 3.5 ml of the same buffer.…”

Section: Extraction Of the Enzymes Involved In Antioxidant Metabolismmentioning

confidence: 99%

Oxidative behaviour of Blanquilla pears treated with 1‐methylcyclopropene during cold storage

et al. 2004

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The increasing use of 1-methylcyclopropene (1-MCP) to extend the commercial life of fruit constitutes an attractive way of improving packing house competitiveness. This compound prevents the effects of ethylene in a wide range of fruit and vegetables. However, despite the extensive literature relating to this action on ethylene, little is known about its other physiological effects. In this work, pears (Pyrus malus L cv Blanquilla) were treated with 100 ppb 1-MCP immediately after harvest and stored in air for 5 months. Differences in oxidative stress and in antioxidant potential between controls and 1-MCP-treated fruits were established, determining the changes in the levels of hydrogen peroxide, ascorbate content and ionic leakage during storage. Activities of the H 2 O 2 -generating enzyme superoxide dismutase (EC 1.15.11) and the H 2 O 2 -scavenging enzymes catalase (EC 1.11.1.6), ascorbate peroxidase (EC 1.11.1.11) and unspecific peroxidase (EC 1.11.1.7) were also determined. 1-MCP-treated fruits exhibited lower levels of hydrogen peroxide, ascorbate and lower ionic leakage during storage. In accordance with this result, 1-MCP-treated fruits also exhibited higher enzymatic antioxidant potential. These results challenge the hypothesis that the beneficial effects of 1-MCP on ripening were not exclusively due to its action on ethylene but also to an increase in antioxidant potential in pear.

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“…In these conditions, AOS attack vital biomolecules and disturb the cell metabolism and ultimately the cell causes its own death (Sakihama et al, 2002). Malondialdehyde (MDA), a decomposition product of polyunsaturated fatty acids hydroperoxides, has been utilized very often as a suitable biomarker for lipid per oxidation (Bailly et al, 1996) which is an effect of oxidative damage. Nonetheless, lipids are not the only targets for MDA action; in fact MDA damages DNA, forming adducts to deoxyguanosine and deoxyadenosine (Marnett, 1999).…”

Section: Introductionmentioning

confidence: 99%

Effect of Potassium Levels on Antioxidant Enzymes and Malondialdehyde Content under Drought Stress in Sunflower (Helianthus annuus L.)

Soleimanzadeh¹

2010

American Journal of Agricultural and Biological Sciences

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Problem statement: Drought stress as a major adverse factor can lower leaf water potential, leading to reduced turgor and some other responses and ultimately lower crop productivity in arid and semi arid zones. Sunflower is one of the main oil seed crops in Iran, where drought stress is the most limiting factor. Drought stress tolerance requires the activation of complex metabolic activities including antioxidative pathways, especially Activated Oxygen Species (AOS) and scavenging systems within the cells which can contribute to continued growth under drought stress. Approach: To evaluate the effect of limited irrigation systems and potassium fertilizer on grain yield, antioxidant enzymes and lipid peroxidation biomarker (MDA), the crop was sown in the Research Farm of College of Agriculture, Islamic Azad University, Karaj Branch in 2009. The experimental treatments were arranged as split plots based on a Randomized Complete Block Design with three replications. The main plots were allocated to three different irrigation regimes. The irrigation regimes comprised of: Full Irrigation (IR 1 ), Moderate drought stress (IR 5 ) and Severe drought stress (IR 2 ). The subplots were allocated to four potassium chemical fertilizer (Potassium nitrate) consisting of K 1 = 25, K 2 = 50, K 3 = 75 and K 4 = 100% recommended. Results: Plants under drought stress and potassium levels showed a significant increase and decrease, respectively, in SOD, CAT and GPX activity and MDA in compared to control plants. In this context, plants with higher levels of potassium showed higher resistance to drought stress conditions and higher yield and dry matter allocation to grain filling process i.e. harvest index. Results of this study suggested that drought stress leads to production of oxygen radicals, which results in increased lipid peroxidation (MDA biomarker) and oxidative stress in the plant. Conclusion: The scavenging of AOS by the scavenging system especially by SOD, CAT and GPX was done well and damage to membranes or MDA was controlled at higher levels of potassium.

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Changes in malondialdehyde content and in superoxide dismutase, catalase and glutathione reductase activities in sunflower seeds as related to deterioration during accelerated aging

Cited by 438 publications

References 35 publications

Organization of lipid reserves in cotyledons of primed and aged sunflower seeds

Organization of lipid reserves in cotyledons of primed and aged sunflower seeds

Oxidative behaviour of Blanquilla pears treated with 1‐methylcyclopropene during cold storage

Effect of Potassium Levels on Antioxidant Enzymes and Malondialdehyde Content under Drought Stress in Sunflower (Helianthus annuus L.)

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