Changes in lipids and free fatty acid fractions in adult Haemonchus contortus during incubation in vitro

Kapur, Jyotika; Sood, M. L.

doi:10.1016/0304-4017(87)90028-8

Cited by 2 publications

(2 citation statements)

References 13 publications

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“…These variations might occur due to the availability of food materials, which is influenced by the food habit of the host, in the different microhabitat of the parasites. Key enzymes of lipid biosynthesis have been observed in Haemonchus contortus (Kapur and Sood 1987), Trichuris globulosa (Sarwal et al 1989) and A. galli (Aggarwal et al 1989). A. galli, being capable of synthesizing lipids into other forms, absorbs less TL than cestode.…”

Section: Discussionmentioning

confidence: 99%

Variation in lipid and fatty acid uptake among nematode and cestode parasites and their host, domestic fowl: host–parasite interaction

Mondal¹,

Kundu

Misra³

2016

J Parasit Dis

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Lipid synthesis is an important process in most organisms as well as in helminths. The present observation shows the variation of lipid and fatty acid uptake among cestode, Raillietina (Fuhrmannetta) echinobothrida; nematode, Ascaridia galli and their host, Gallus domesticus, the common country fowl. Total lipid (TL), neutral lipid (NL), glycolipid (GL), phospholipid (PL) and their fatty acid of cestode, nematode and liver and intestinal fluid of the host were analyzed by thin layer chromatography and gas liquid chromatography respectively. The result shows that liver take more TL, PL and GL except NL. Utilization of lipid from intestinal fluid when compare between the parasites, it is found that TL and PL content of cestode are higher than nematode, whereas, nematode absorbs more NL and GL than cestode. The percent of cholesterol is more in cestode than nematode. Palmitic, stearic, oleic and linoleic are the predominant fatty acids among all the samples. The present study reveals that the cestode having large surface area is more opportunistic in the resource utilization over the nematode as well as the host.

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Section: Discussionmentioning

confidence: 99%

Variation in lipid and fatty acid uptake among nematode and cestode parasites and their host, domestic fowl: host–parasite interaction

Mondal¹,

Kundu

Misra³

2016

J Parasit Dis

View full text Add to dashboard Cite

show abstract

“…Development of suitable chemotherapeutic agents for effective control of parasites depends on our understanding of the composition of biochemical entities and metabolism of the latter and also the differences with the host tissues, if any. However, such information for parasitic nematodes is limited, although certain aspects of lipid metabolism have been worked out for other nematodes, such as Dirofilaria immitis (HUTCHISON et al, 1976), Ascaris lumbricoides (BARRETT et al, 1970), Caenorhabditis briggsae and Panagrellus redivivus (ROTHSTEIN, 1970), Setaria cervi (ANSARI et al, 1973), Litomosoides carinii (SUBRAHAMANYAM, 1967) and Haemonchus contortus (KAPOOR & SOOD, 1987). In most of these studies, however, data on qualitative lipid analysis of the parasite is presented without providing sufficient insight into the dynamics of lipid metabolism.…”

Section: Introductionmentioning

confidence: 99%

Lipid metabolism inTrichuris globulosa(Nematoda)

1989

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Adult males and females ofTrichuris globulosa, an intestinal nematode parasite of goats, were studied for their lipid composition, capability of incorporation of (Na)-1-14C-acetate into different lipid classes and the activity of certain key enzymes of lipid metabolism. The parasite possesses a large variety of lipids including certain complex lipids. These are phosphatidylcholine (PC), diphosphatidylglycerol (cardiolipin), lysophosphatidylcholine (LPC), lysophosphatidylethanolamine (LPE), phosphatidylserine (PS), phosphatidylinositol (PI), plasmalogens (choline+ethanolamine), mono-, di- and triacylglycerols, free and esterified cholesterol, non-esterified fatty acids (NEFA), gangliosides, cerebrosides (glycosyl ceramide) and sulphuric acid esters of cerebrosides (sulphatides). The females contain more lipids than males, particularly the acylglycerols and phospholipids, possibly to meet the energy requirement and structural entities for the daily production of large numbers of eggs. Incorporation studies of labelled substrate, sodium-1-14C acetate demonstrate that the adult female has extremely active mechanisms for biosynthesizing these lipids. Most of the labels are found in PC, PE, SM, acylglycerols, NEFA, gangliosides, cerebrosides and sulphatides. Cholesterol, although a minor component of the parasitic lipids, incorporates large amount of label and also undergoes fast turnover. Kinetic analysis of the incorporation by measuring the rate constant (k) and half life (t½) reveals that gangliosides are the fastest biosynthesizing and turning over lipids, although they constitute only 0·1% of the total lipids. The presence of important enzymes of lipid biosynthesis, glucose-6-phosphate dehydrogenase, malate dehydrogenase and hydroxymethyl glutaryl-CoA reductase and an enzyme of lipid ester hydrolysis, triacylglycerol lipase, is also established inT. globulosa. Michaelis-Menten kinetic characteristics of the parasitic enzymes (Km, Vmax, v and the first order rate constant, k) are comparable with those of rat liver homogenates.

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Changes in lipids and free fatty acid fractions in adult Haemonchus contortus during incubation in vitro

Cited by 2 publications

References 13 publications

Variation in lipid and fatty acid uptake among nematode and cestode parasites and their host, domestic fowl: host–parasite interaction

Variation in lipid and fatty acid uptake among nematode and cestode parasites and their host, domestic fowl: host–parasite interaction

Lipid metabolism inTrichuris globulosa(Nematoda)

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