Changes in lignocellulolytic enzyme activites in six Pleurotus spp. strains cultivated on coffee pulp in confrontation with Trichoderma spp.

Mata, Gerardo; Hernández, Dulce María Murrieta; Iglesias-Andreu, Lourdes Georgina

doi:10.1007/s11274-004-3041-3

Cited by 40 publications

(32 citation statements)

References 29 publications

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“…This fact could be explained by several issues like the efficiency of the extraction method employed; changes in the composition of the substrate during the fermentation since new compounds inhibiting the enzymes can be formed; repression exerted by the products generated during the enzymatic reactions; and synthesis of several isoenzymatic forms of the same enzymes at different times under different substrate conditions leading to different activities. This reduction in the enzyme activities has also been observed by other authors who have cited some of the above-mentioned causes for such decline (Mata et al, 2005).…”

Section: Time Course Of Enzyme Production During Ssfsupporting

confidence: 83%

Production of lignocellulolytic enzymes from three white-rot fungi by solid-state fermentation and mathematical modeling

Montoya¹,

Levin²

2015

Afr. J. Biotechnol.

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Three species of white rot-fungi (Pleurotus ostreatus, Coriolus versicolor, and Lentinula edodes) were grown on 12 solid media based on several lignocellulosic materials (oak sawdust, coconut husks, coffee husks and corn bran) during 49 days. The media had varied carbon/nitrogen ratios and CuSO 4 content. The objective of the work was to evaluate the effect of the media formulation on the production of lignocellulolytic enzymes and degradation of lignocellulosic components by the three fungal species. C. versicolor exhibited the highest ability to degrade the three main polymers of the lignocellulosic waste materials employed and to produce ligninases with titers as high as 107 U/g solid substrate in the case of laccase. In addition, a mathematical model describing the fermentation kinetics of the cell biomass growth, degradation of lignocellulosic components, and lignocellulolytic enzyme production for the fungal species/medium combination exhibiting the best performance under solid-state fermentation conditions was proposed and validated in the case of C. versicolor. The mathematical model could be used to provide valuable information on the process itself as well as to contribute to the development of a future commercial process for lignocellulolytic enzyme production.

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Section: Time Course Of Enzyme Production During Ssfsupporting

confidence: 83%

Production of lignocellulolytic enzymes from three white-rot fungi by solid-state fermentation and mathematical modeling

Montoya¹,

Levin²

2015

Afr. J. Biotechnol.

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“…The only exception to this trend occurred when the Pleurotus strains were placed in confrontation with T. reesei. (Mata et al 2005). In this case, laccase production remained the same until day 16.…”

Section: Discussionmentioning

confidence: 74%

“…Furthermore, they were 2-25 times higher than in pure cultures depending on the species. Laccase activity peaks and the effect of interspecies interactions have also been observed in situ (Mata et al 2005;Velázquez-Cedeño et al 2004). For instance, laccase production during the vegetative growth of six Pleurotus strains in confrontation with three Trichoderma strains cultured on coffee pulp, increased at day 7, showing its maximum value at day 12.…”

Section: Discussionmentioning

confidence: 81%

Interspecific interactions with Trichoderma longibrachiatum induce Pleurotus ostreatus defence reactions based on the production of laccase isozymes

et al. 2007

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Laccases are phenoloxidases involved in aromatic compound transformation but also in stress response towards antagonist species such as Trichoderma sp. In this study intracellular isoforms of laccases produced by Pleurotus ostreatus in liquid cultures with or without Trichoderma longibrachiatum showed five isoforms with various intensities depending on the culture conditions suggesting a basal expression of these enzymes, which can be induced by interspecific interactions. A first attempt to analyse the induction of P. ostreatus laccase-gene expression by a biotic factor was realized using semi-quantitative RT-PCR. We showed that the transcription of a laccase gene of P. ostreatus can be modified by a biotic stress such as T. longibrachiatum.

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“…Therefore, it has been hypothesized that the mixed fungal cultures may result in an efficient pretreatment of woody biomass through synergistic interactions. Previous investigations regarding fungal co-cultures have mainly focused on their interactions with each other and the production of enzymes, as well as lignocellulosic degradation (Baldrian 2004;Boddy 2000;Chi et al 2007;Iakovlev and Stenlid 2000;Mata et al 2005;Savoie and Mata 1999;Score et al 1997). Ma et al (2011) have also reported the influences of a co-fungal culture of Auricularia polytricha AP with Irpex lacteus CD2 on the pyrolysis characteristics of corn stover.…”

Section: Introductionmentioning

confidence: 99%

Biological Pretreatment with White Rot Fungi and Their Co-Culture to Overcome Lignocellulosic Recalcitrance for Improved Enzymatic Digestion

Wang¹,

Yuan²,

BaoKai³

2014

BioResources

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Three white rot fungi (Lenzites betulinus, Trametes orientalis, and Trametes velutina) as well as their respective paired cultures were used to pretreat Populus tomentosa for enhanced lignocellulosic degradation and enzymatic hydrolysis. Hemicellulose and cellulose were slightly degraded, while a maximum lignin degradation of 58% was caused by T. velutina during the 12-week cultivation. After the pretreated samples were subjected to enzymatic hydrolysis for 96 h, the reducing sugar released by T. orientalis at week 12 was as high as 41%, which was in line with the lignin loss at 2.2 times the control sample. Overall, the monocultures of white-rot fungi exhibited better degradation and saccharification of woody biomass than their co-culture. This can be attributed to the partial removal of lignin and hemicellulose, with an associated increase of cellulose accessibility to enzymes.

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Changes in lignocellulolytic enzyme activites in six Pleurotus spp. strains cultivated on coffee pulp in confrontation with Trichoderma spp.

Cited by 40 publications

References 29 publications

Production of lignocellulolytic enzymes from three white-rot fungi by solid-state fermentation and mathematical modeling

Production of lignocellulolytic enzymes from three white-rot fungi by solid-state fermentation and mathematical modeling

Interspecific interactions with Trichoderma longibrachiatum induce Pleurotus ostreatus defence reactions based on the production of laccase isozymes

Biological Pretreatment with White Rot Fungi and Their Co-Culture to Overcome Lignocellulosic Recalcitrance for Improved Enzymatic Digestion

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