Changes in Levels of Immunoreactive Prolactin Isoforms during a Reproductive Cycle in Turkey Hens

Bédécarrats, Grégoy Y.; Guemene, Daniel D.; Kühnlein, U.; Zadworny, D.

doi:10.1006/gcen.1998.7187

Cited by 24 publications

(8 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For the first time in avian species, different immunoreactive forms of PRL have been detected in the pituitary gland of embryos and poults during development. The isoforms of PRL detected by Western blotting correspond to those previously detected in pituitary gland from adult turkey hens [29]. These isoforms are consistent with the G-and NG-PRL isoforms described by Corcoran and Proudman [28] with the 27-kDa and 24-kDa forms corresponding to the G-and NG-PRL forms, respectively.…”

Section: Discussionsupporting

confidence: 81%

“…The NG-PRL isoform was predominant 5 days before hatching, then the ratio G-PRL:NG-PRL was close to 1 around hatching, and finally the G-PRL appeared to be predominant at 1 wk of age. We previously reported that, in pituitary glands from adult turkey hens, higher percentages of G-PRL were associated with high levels of total PRL, and lower percentages were associated with low levels of total PRL content [29]. In addition, by using an in vitro perifusion system, we demonstrated that both G-and NG-PRL isoforms are released by the pituitary gland from adult turkey hens in the same relative proportion as observed in the pituitary extracts [30].…”

Section: Discussionmentioning

confidence: 56%

“…Prestained broad-range (M r 6.5-175 [ϫ 10 Ϫ3 ]) molecular weight markers (New England Biolabs LTD, Beverly, MA) were included in each gel. A 10-g protein sample from pooled pituitary extracts from adult turkey hens at different physiological stages [29] was also included as positive control. After electrophoresis, the proteins were electrotransferred onto a polyvinylidene fluoride (PVFD) membrane (Immobilon-P, 0.45 m; Millipore Corp., Bedford, MA) at constant voltage of 100 V for 1 h at 4ЊC with constant stirring in 25 mM Tris, 192 mM glycine, and 20% methanol, pH 8.3.…”

Section: Analysis Of Prl Immunoreactive Isoformsmentioning

confidence: 99%

“…In turkey hens, PRL is present in the pituitary gland in 3 different isoforms, 1 nonglycosylated (NG)-PRL and 2 G-PRLs, which comigrate on SDS-PAGE [28]. The ratio of G-PRL to NG-PRL has been shown to fluctuate with the reproductive status of the animal [29]. Higher percentages of G-PRL are associated with high levels of total PRL (during incubation behavior), and higher percentages of NG-PRL are associated with low levels of total PRL (during photorefractoriness and molting).…”

Section: Introductionmentioning

confidence: 98%

See 3 more Smart Citations

Quantification of Prolactin Messenger Ribonucleic Acid, Pituitary Content and Plasma Levels of Prolactin, and Detection of Immunoreactive Isoforms of Prolactin in Pituitaries from Turkey Embryos During Ontogeny1

Bédécarrats¹,

Guemene²,

Morvan³

et al. 1999

Biology of Reproduction

Self Cite

View full text Add to dashboard Cite

The content of prolactin mRNA as well as total prolactin content and type of isoforms of prolactin were measured in single pituitary glands from turkey embryos and poults. Levels of mRNA and pituitary content of prolactin remained low until 5 days before hatching, while plasma concentrations remained low until 2 days before hatching. Levels of prolactin mRNA then increased until the day of hatch, stayed stable during the 3 first days of age, and significantly increased until 2 wk of age. Similar changes were observed in pituitary content and plasma levels of prolactin. Two immunoreactive bands of apparent molecular masses of 24 and 27 kDa, corresponding to the nonglycosylated and glycosylated form of prolactin, respectively, were visualized on Western blots. In pituitary glands from embryos at 22 days of incubation, 31.5% of the protein was glycosylated, whereas in embryos at 27 days of incubation and poults at 1 and 7 days of age, 48.6%, 48.0%, and 56. 0% of prolactin was glycosylated, respectively. The results indicate that the increases in the synthesis and the release of prolactin occur mainly around and after the time of hatching in the turkey embryo. Higher percentages of glycosylated isoforms were associated with increasing levels of total prolactin in the pituitary gland. Thus, the synthesis of prolactin and its post-translational modifications may be important factors involved in the physiologic changes occurring around the time of hatching.

show abstract

Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 56%

Section: Analysis Of Prl Immunoreactive Isoformsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

See 2 more Smart Citations

Quantification of Prolactin Messenger Ribonucleic Acid, Pituitary Content and Plasma Levels of Prolactin, and Detection of Immunoreactive Isoforms of Prolactin in Pituitaries from Turkey Embryos During Ontogeny1

Bédécarrats¹,

Guemene²,

Morvan³

et al. 1999

Biology of Reproduction

Self Cite

View full text Add to dashboard Cite

show abstract

“…The rPRL could effectively inhibit broodiness in Dwarf chickens but does not significantly affect egg production (Zadworny et al, 1988). Bédécarrats et al (1999) found that immunisation with rPRL also effectively inhibits broodiness without significantly affecting the total egg production in turkeys.…”

Section: Discussionmentioning

confidence: 97%

Prokaryotic Expression and Active Immunisation against Recombinant-Derived Prolactin Fusion Protein on Broodiness and Egg-Laying in Wanxi-White Goose

Chen

Wang

et al. 2015

Avian Biology Research

View full text Add to dashboard Cite

This experiment aims to produce a recombinant prolactin (rPRL) for use in immunising Wanxi-white goose and to determine the immunisation effects on broodiness and egg production. A stable prokaryotic expression system with rPRL obtained extra-cellularly was established. Wanxi-white geese (n = 100) performed broodiness were randomly divided into five groups (20 per group): group I was injected with saline as negative control, group II was orally administered with Bromocriptine as positive control, groups III, IV and V were subcutaneously injected with either 1, 2 or 4 mg·mL -1 rPRL, respectively. This process (FA-I) was repeated after interval of 10 days (SA-I). After FA-I, broodiness significantly decreased in groups III, IV and V compared to group I (P < 0.01), while egg production showed no significant difference among groups. After SA-I, broodiness in group IV and V was significantly lower than that of other groups (P < 0.05), and egg production percentage in group IV showed significantly higher than that of group III (P <0.05), but only numerically higher than that of other groups. Broodiness showed no difference among groups 10 days after SA-I, but broodiness was significantly increased to more than 20% in all groups. Serum prolactin concentrations showed high secretion in geese under broodiness and decreased in geese that wake up from broodiness after SA-I. In conclusion, active immunisation with rPRL decreased broodiness and increased egg production in Wanxi-white geese. However, the immune period was short, and the performance was significantly affected by environmental factors.

show abstract

In VitroRelease of Isoforms of Prolactin from Pituitary Glands of Turkey Hens at Different Physiological Stages

Bédécarrats

Guemene²,

Morvan³

et al. 1999

General and Comparative Endocrinology

View full text Add to dashboard Cite

Changes in Levels of Immunoreactive Prolactin Isoforms during a Reproductive Cycle in Turkey Hens

Cited by 24 publications

References 42 publications

Quantification of Prolactin Messenger Ribonucleic Acid, Pituitary Content and Plasma Levels of Prolactin, and Detection of Immunoreactive Isoforms of Prolactin in Pituitaries from Turkey Embryos During Ontogeny1

Quantification of Prolactin Messenger Ribonucleic Acid, Pituitary Content and Plasma Levels of Prolactin, and Detection of Immunoreactive Isoforms of Prolactin in Pituitaries from Turkey Embryos During Ontogeny1

Prokaryotic Expression and Active Immunisation against Recombinant-Derived Prolactin Fusion Protein on Broodiness and Egg-Laying in Wanxi-White Goose

In VitroRelease of Isoforms of Prolactin from Pituitary Glands of Turkey Hens at Different Physiological Stages

Contact Info

Product

Resources

About