Changes in lectin-binding sites on epididymal cells during postnatal development of the mouse

Tajiri, Shota; Fukui, Tatsuya; Sawaguchi, Akira; Yoshinaga, Kazuya

doi:10.2535/ofaj.88.153

Cited by 3 publications

(5 citation statements)

References 22 publications

(11 reference statements)

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“…This observation suggests that functional differentiation (maturation) of basal cells is complete at this stage, which is just before spermatozoa begin to reach the epididymis. A similar phenomenon has been reported in previous studies 6,20,22) .…”

Section: Discussionsupporting

confidence: 92%

“…We also demonstrated temporal and spatial differences in CK5 and CK14 expression with age and between regions, which may reflect differences in the functions of basal cells during postnatal development. Similar changes, associated with the development and differentiation of epididymal basal cells, have been observed in our previous studies on the structure of cellular sugar chains 19,20) .…”

Section: Discussionsupporting

confidence: 88%

“…We designated these cells the flask-shaped basal cells. To determine whether the flask-shaped basal cells can cross the tight-junction barrier, double labeling for GS-IB4 lectin, which is a marker of basal cells 19,20) , and tight junction protein ZO-1 21) , was performed on mouse epididymis. clearly shows a flask-shaped basal cell extending its body projection to cross the ZO1-labeled tight junction barrier and toward the lumen.…”

Section: Ck5 and Ck14 Are Markers Of Epididymal Basal Cellsmentioning

confidence: 99%

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Cytokeratin localization and basal cell differentiation in the epididymal epithelium during postnatal development of the mouse

Yoshida

Murakami

Sakuda

et al. 2015

Okajimas Folia Anat. Jpn.

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The epididymis is a male genital organ that has plays various functions, including sperm concentration, maturation, and storage. The epididymal epithelium consists of principal cells, clear cells, and basal cells. To comprehensively understand the occurrence and morphological differentiation of basal cells, we examined the expression and localization of cytokeratins (CKs) in the epididymal epithelium during postnatal development of the mouse. Immunohistochemical staining showed that, in adult mice, CK5 and CK14 were exclusively expressed in the cytoplasm of basal cells. During postnatal development, basal cells that stained positive for CK5 and CK14 first appeared in immature columnar epithelial cells in mice aged 1 week. The immunoreactivity became progressively stronger in mice aged 2-3 weeks. In mice aged 3 weeks, the immunoreactivity was strong in regions IV and V. In mice aged ≥ 4 weeks, strong immunoreactivity was observed in all epididymal regions. CK5 and CK14 could be useful markers of differentiation in epididymal basal cells. These basal cells originate from immature columnar epithelial cells and are of two types—dome-shaped and flask-shaped—. The flask-shaped cells are mainly located in the initial segment of the mouse epididymis.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 88%

Section: Ck5 and Ck14 Are Markers Of Epididymal Basal Cellsmentioning

confidence: 99%

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Cytokeratin localization and basal cell differentiation in the epididymal epithelium during postnatal development of the mouse

Yoshida

Murakami

Sakuda

et al. 2015

Okajimas Folia Anat. Jpn.

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“…5). The Arl13b-Cetn2 tg mouse model displays the same pattern of epididymis development as previously described in wild-type mice [12] (Fig. 5, Top).…”

Section: Resultsmentioning

confidence: 58%

“…The differentiation of epididymal cell populations and the establishment of distinct epididymal segments was studied in different species during the 80’s, including in mice, rats and humans [1, 4–11]. It consists of three main stages: the undifferentiated period, the period of differentiation, and the period of expansion [12]. During the undifferentiated period (1 st week in mouse), the epididymis epithelium is characterized by the presence of columnar cells that lack stereocilia and form primitive junctions.…”

Section: Introductionmentioning

confidence: 99%

Cell-lineage specificity of primary cilia during epididymis post-natal development

Bernet

Bastien

Soulet

et al. 2018

Preprint

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30Primary cilia are sensory organelles that orchestrate major signaling pathways during organ 31 development and homeostasis. By using a double Arl13b/mCherry-Cetn2/GFP transgenic mouse 32 model, we characterized the spatio-temporal localization of primary cilia in the epididymis, from 33 birth to adulthood. We report here a constitutive localisation of primary cilia in peritubular 34 myoid cells and a dynamic profiling in differentiated epithelial cells throughout post-natal 35 development. While primary cilia are present at the apical pole of the undifferentiated epithelial 36 cells from birth to puberty, they are absent from the apical pole of the epithelium in adults, 37where they appear exclusively associated with cytokeratin 5-positive basal cells. Exogenous 38 labeling of primary cilia marker Arl13b and IFT88 confirmed the cell lineage specific localization 39 of primary cilia in basal cells and myoid cells in human epididymides. From whole epididymis 40 tissues and serum-free cultures of DC2 murine epididymal principal cell lines we determined 41 that primary cilia from the epididymis are associated with the polycystic kidney disease-related 42 proteins polycystin 1 (PC1) and polycystin 2 (PC2), and Gli3 Hedgehog signaling transcription 43 factor. Thus, our findings unveil the existence of primary cilia sensory organelles, which have the 44 potential to mediate mechano/ chemo-signaling events in the epididymis. 45

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Cell-lineage specificity of primary cilia during postnatal epididymal development

et al. 2018

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Changes in lectin-binding sites on epididymal cells during postnatal development of the mouse

Cited by 3 publications

References 22 publications

Cytokeratin localization and basal cell differentiation in the epididymal epithelium during postnatal development of the mouse

Cytokeratin localization and basal cell differentiation in the epididymal epithelium during postnatal development of the mouse

Cell-lineage specificity of primary cilia during epididymis post-natal development

Cell-lineage specificity of primary cilia during postnatal epididymal development

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