Changes in Human Natural Killer Activity Early and Late After Renal Transplantation Using Conventional Immunosuppression

Guillou, Pierre J.; Hegarty, J; Ramsden, C. H.; Davison, A. M.; Will, Eric J.; Giles, G. R.

doi:10.1097/00007890-198204000-00015

Cited by 41 publications

(7 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The results ofthe present study demonstrate that riatural killer (NK) cell activity is depressed for a short time after open-heart surgery. Similar changes in NK activity have rccently been found in surgical patients operated on without the aid of cardiopulmonary bypass (14). Whether this depression of NK activity is due to a selective decrease in the number of thcse cytotoxic cells in peripheral blood or to the specific inhibition of their function i s unknown.…”

Section: Discussionmentioning

confidence: 62%

Natural Killer Cell Activity After Open‐Heart Surgery

Ryhänen

Huttunen

Ilonen

1984

Acta Anaesthesiol Scand

View full text Add to dashboard Cite

We studied the effects of elective open-heart surgery with cardiopulmonary bypass on peripheral blood natural killer (NK) cell activity in 12 patients with heart disease. Separated mononuclear cells from patients and control cells taken from healthy volunteers were incubated in microtiter plates for 24 h with 3H-thymidine-labelled K 562 cells as target cells. In this test system, higher counts per minute (cpm) values represent a greater number of surviving target cells and thus weaker NK activity. Results of cultures prepared from blood samples taken preoperatively were compared with those taken 2, 7 and 14 days postoperatively. NK cell activity was depressed (P less than 0.01) for 2 days after surgery. NK cell activity in the control samples did not change significantly. The results show an impairment of NK cell activity immediately after open-heart surgery.

show abstract

Section: Discussionmentioning

confidence: 62%

Natural Killer Cell Activity After Open‐Heart Surgery

Ryhänen

Huttunen

Ilonen

1984

Acta Anaesthesiol Scand

View full text Add to dashboard Cite

show abstract

“…Under standard immunosuppressive therapy, an increase in NK cell activity (50) and NK cell traffic into the liver graft has been observed immediately after liver transplantation (51). Despite the continued administration of conventional immunosuppressive agents, NK cell function has been found to recover in renal transplantation (52). However, NK allo-specific constellations did not seem to play a major role in acute hepatic allograft rejection (53); rather, they were shown to play a role in the recovery from CMV infection in a substantial number of renal transplant patients (54).…”

Section: Discussionmentioning

confidence: 99%

GM-CSF Restores Innate, But Not Adaptive, Immune Responses in Glucocorticoid-Immunosuppressed Human Blood In Vitro

Lucas

Schuchmann³

et al. 2003

The Journal of Immunology

View full text Add to dashboard Cite

Infection remains the major complication of immunosuppressive therapy in organ transplantation. Therefore, reconstitution of the innate immunity against infections, without activation of the adaptive immune responses, to prevent graft rejection is a clinically desirable status in transplant recipients. We found that GM-CSF restored TNF mRNA and protein expression without inducing IL-2 production and T cell proliferation in glucocorticoid-immunosuppressed blood from either healthy donors or liver transplant patients. Gene array experiments indicated that GM-CSF selectively restored a variety of dexamethasone-suppressed, LPS-inducible genes relevant for innate immunity. A possible explanation for the lack of GM-CSF to restore T cell proliferation is its enhancement of the release of IL-1βR antagonist, rather than of IL-1β itself, since exogenously added IL-1β induced an IL-2-independent Con A-stimulated proliferation of glucocorticoid-immunosuppressed lymphocytes. Finally, to test the in vivo relevance of our findings, we showed that GM-CSF restored the survival of dexamethasone- or cyclosporine A-immunosuppressed mice from an otherwise lethal infection with Salmonella typhimurium. In addition to this increased resistance to infection, GM-CSF did not induce graft rejection of a skin allotransplant in cyclosporine A-immunosuppressed mice. The selective restoration potential of GM-CSF suggests its therapeutic use in improving the resistance against infections upon organ transplantation.

show abstract

“…Quanto à imunidade celular, foram realizados: (a) subpopulações de 22 . O critério de normalidade adotado foi determinado por curvas obtidas a partir da análise de uma população normal, padronizadas no próprio laboratório.…”

Section: Métodosunclassified