Objective: To evaluate the influence of strabismus surgery on quality of life (QoL) in Graves' orbitopathy (GO) patients. Design: Prospective study of case series. Methods: Consecutive GO-patients who were scheduled for their first strabismus surgery were included in the study. The patients completed the GO-QoL questionnaire within 3 months before the surgery and 2-4 months after the surgery. A complete orthoptic examination, including the field of binocular single vision (BSV), was performed. Clinically relevant response (CRR) in the QoL was also evaluated. Results: In this study, 28 patients were included. The GO-QoL score for visual functioning was 46.3G24.2 before surgery and 65.7G30.5 after surgery (PZ0.009). The GO-QoL score for appearance changed from 60.6G25.9 to 69.5G24.2 (PZ0.005). After surgery, the field of BSV increased from 24.3G34.8 to 68.5G36.0 points (PZ0.000). A weak correlation was found between the field of BSV and the visual functioning score after surgery (rZ0.417; PZ0.034). CRR was found in 20 (71%) patients. Those with a CRR showed a larger field of BSV (PZ0.002) and better GO-QoL scores (PZ0.008). Conclusions: GO-QoL score increases significantly for both visual functioning and appearance after the first strabismus surgery in GO-patients, showing the highest improvement for the visual functioning questions. Both the GO-QoL and field of BSV outcomes correlate well with the CRR.