Changes in glycolipid glycosyltransferases and glutamate decarboxylase and their relationship to differentiation in neuroblastoma cells

Moskal, Joseph R.; Gardner, David A.; Basu, Subhash

doi:10.1016/0006-291x(74)91021-3

Cited by 53 publications

(5 citation statements)

References 23 publications

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“…(1980) showed a large increase in the synthesis of GMl and GDla in mouse neuroblastoma N N cells treated (Cieselski-Treska e t al., 1977) with the cell-cycleblocking agent 5'-bromo-2-deoxy uridine (BrdU), which they attributed to a n induction of GM,:GalNAc transferase activity. Other studies have shown that treatment of mouse neuroblastoma (NIE-115) cells with dibutyryl cyclic AMP caused a doubling in the incorporation of labeled N-acetylneuraminic acid into G,, (Moskal et al, 1974), and butyrate treatment of H e L a and K B cells, which presumably blocks cells late in the G I phase (Fallon and Cox, 1979), specifically induced lactosylceramide sialyltransferase activity (Simmons et al, 1975;Macher e t al., 1978). However, there have been no detailed studies on the regulation of ganglioside metabolism during the cell cycle of any cells of neural origin.…”

Section: A Scheideler Et Almentioning

confidence: 97%

Cell‐Cycle Dependence of a Ganglioside Glycosyltransferase Activity and Its Inhibition by Enkephalin in a Neurotumor Cell Line

Scheideler

Lockney

Dawson

1984

Journal of Neurochemistry

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Rat glioma X mouse neuroblastoma hybrid neurotumor cells (NG108-15), synchronized by amino acid deprivation, showed a cell-cycle-dependent peak of activity of a ganglioside N-acetylgalactosaminyl transferase 14-24 h following release from the cell cycle block (S/G2 phase). Maximal expression of two typical lysosomal hydrolases, N-acetyl-beta-hexosaminidase and beta-galactosidase, occurred between 18 and 21 h following release (S phase), declining to G1 phase levels during the peak of N-acetylgalactosamine (GalNAc) transferase activity. In addition, glycosyltransferase activity in G2 phase cells showed an increase in apparent Vmax (suggesting the presence of more enzyme/mg of cell protein) and apparent binding affinity for uridine diphosphate N-acetylgalactosamine (UDP-GalNAc) (32 versus 14 microM) when compared to transferase activity in the G1 phase. However, the opioid peptide enkephalin [D-Ala2, D-Leu5], which inhibits ganglioside GalNAc transferase activity in unsynchronized NG108-15 cultures, was much more inhibitory in whole cells 8 h after release from the cell cycle block (G1 phase) than in cells 20 h after release (G2 phase), with 50% inhibition occurring at 2 X 10(-9) M and 2 X 10(-7) M, respectively. These results suggest that the GalNAc transferase activity is regulated in more than one way during the cell cycle, since both Vmax and Km changes are observed, and that the cyclic AMP-dependent mechanism by which opiates reduce transferase activity is receptor mediated and cell cycle dependent.

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Section: A Scheideler Et Almentioning

confidence: 97%

Cell‐Cycle Dependence of a Ganglioside Glycosyltransferase Activity and Its Inhibition by Enkephalin in a Neurotumor Cell Line

Scheideler

Lockney

Dawson

1984

Journal of Neurochemistry

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“…It is of interest that, in spite of the presence ofsomesialyltransferases [Moskal et a/., 1974], neither neuroblastoma clones subjected to various stimuli [Ditffarcl et aL, 1977;Dawson and Stoolmiller, 1976;Ciesielski-Treska et al, 1977] nor cultured astroblasts [Robert et al, 1975] display the capacity to accumulate tri sialo-and tetrasialogangliosides in vitro.…”

Section: Discussionmentioning

confidence: 99%

Ganglioside Profiles during Neural Tissue Development

Yavin

1979

Dev Neurosci

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The accumulation and species distribution of gangliosides in cultured cerebral cells and in the late prenatal stage of the developing rat brain were examined as a function of their growth state. The concentration of lipid-bound sialic acid in cerebral cells rises from a value of about 5 nmol/mg protein at day 1 to a peak level of 16.2 nmol/mg protein by 12 days, followed by a substantial decrease after 25 days in culture. When expressed per cell DNA, a linear increase of about 10-fold, notably after 10 days in culture, in the amount of sialic acid is seen. The most significant change in the species distribution is an apparent accumulation of disialo ganglioside (G_D1a) trisialo ganglioside (G_T) and tetrasialo ganglioside (G_Q), whereas a marked reduction of G_D3 is evident. The accumulation of sialic acid and the developmental patterns of the ganglioside species in vitro reasonably agree with the developing prenatal brain. Thus, by birth, both G_T and G_Q account for about 36% of the total brain gangliosides as compared to 40% in the mature cerebral cultures. Since in the newborn rat brain synaptogenesis is not prominent the increase in the ganglioside complexity could be associated with neuritic sprouting and establishment of nonsynaptic interneuronal connections. Rabbit antiserum raised against pure G_D1a reacted preferentially with the neuronal cell bodies and neurite extensions as observed by an indirect immunofluorescence technique. A progressive increase in immunofluorescence labeling paralleled accumulation of polysialo-gangliosides and culture maturation. In this context, the dissociated cerebral cells provide a powerful tool for investigating aspects of neuronal development because of the possibility of correlating neurochemical data with ultrastructure and cell-surface-related events.

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“…Gangliosides are cell membrane-associated molecules that play a role in a variety of cellular events, including differentiation (1)(2)(3), defense (4), growth (3,5,6), regeneration (7), and transformation (7). The striking changes in the distribution and quantity of gangliosides during cephalogenesis (8) suggest that they are important in neuronal development.…”

mentioning

confidence: 99%

Ganglioside Stimulation of Axonal Sprouting in Vitro

Roisen

Bartfeld

Nagele

et al. 1981

Science

379

102

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Bovine brain gangliosides were applied to primary and established neuronal cultures to examine the role of gangliosides in neuronal development. Media containing gangliosides enhanced the degree of axonal elongation exhibited by sensory ganglia neurons and increased the length and number of Neuro-2a neuroblastoma cell processes. Ganglioside-supplemented media caused a twofold increase in ornithine decarboxylase activity in both culture systems. These experiments suggest that gangliosides function as acceptor molecules for growth-promoting substances in embryonic and tumor-derived neurons.

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Changes in glycolipid glycosyltransferases and glutamate decarboxylase and their relationship to differentiation in neuroblastoma cells

Cited by 53 publications

References 23 publications

Cell‐Cycle Dependence of a Ganglioside Glycosyltransferase Activity and Its Inhibition by Enkephalin in a Neurotumor Cell Line

Cell‐Cycle Dependence of a Ganglioside Glycosyltransferase Activity and Its Inhibition by Enkephalin in a Neurotumor Cell Line

Ganglioside Profiles during Neural Tissue Development

Ganglioside Stimulation of Axonal Sprouting in Vitro

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