Ganglioside Stimulation of Axonal Sprouting in Vitro

Roisen, Fred J.; Bartfeld, Harry; Nagele, Robert G.; Yorke, G.

doi:10.1126/science.7291999

Cited by 379 publications

(112 citation statements)

References 17 publications

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“…Gangliosides are known to induce the differentiation of neuroblastoma cell lines with concomitant sprouting and extension of neurites [1,2], although the underlying molecular mechanism remains unknown [3,4]. Since others have shown that gangliosides are inhibitors of protein kinase C [5,6], it seemed possible that the two findings were related and that gangliosides might stimulate neuritogenesis by inhibiting the activity of protein kinase C. To check this hypothesis, we have tested the effect of 1-(5-isoquinolinylsulfonyl)-2-methylp~perazine (H7), a specific inhibitor of protein kinase C [7], on neurite outgrowth of neuro 2a cells.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of protein kinase C induces differentiation of neuroblastoma cells

1989

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It is shown that 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H7), a specific inhibitor of protein kinase C, induces neuritogenesis in neuro 2a cells. The percentage of differentiated cells was 9%, 20%, 59% and 85% at 0, 17, 85 and 500 ~tM H7, respectively. The number of neurites/cell increased 2-, 8-and 14-fold over the controls for 17, 85 and 500 gM H7, respectively. These results indicate that protein kinase C plays a key role in the control of differentiation of neural cells and that its specific inhibition may be of basic as well as of practical importance.

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Section: Introductionmentioning

confidence: 99%

Inhibition of protein kinase C induces differentiation of neuroblastoma cells

1989

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“…GM1 therapy resulted in signi®cantly improved functional recovery. 23 ± 27 Therefore, gangliosides are used as neuroprotective agents for the treatment of spinal cord injury 25 as well as ischemia and degenerative diseases.…”

Section: Introductionmentioning

confidence: 99%

Ganglioside patterns in human spinal cord

Vorwerk

2001

Spinal Cord

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Objective: To examine the distribution of gangliosides in human cervical and lumbar spinal cord. Setting: Magdeburg, Germany. Methods: The ganglioside distribution of human cervical and lumbar spinal cord enlargements from 10 neurological normal patients was analyzed. Gangliosides were isolated from di erent areas corresponding to the columna anterior, columna lateralis and columna posterior. Results: Ganglioside GfD1b/GD1b and GD3 were the most abundant gangliosides in all examined tissues. The total concentration of sialic acid bound gangliosides GM2 and GM3 was less than 5%. The GD3 fraction constantly consisted of a double band as assessed by TLC after lipid extraction. There were signi®cant di erences in the ganglioside distribution when comparing tissue from the columna anterior, columna lateralis and columna posterior of the lumbar enlargement of the spinal cord.

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“…The expression patterns of gangliosides change during neuronal differentiation and development (2,3,6). Several studies have reported that the expression of gangliosides is regulated during nervous system development and is closely associated with neurogenesis, neural repair, and synaptic function (7)(8)(9)(10)(11)). Moreover, the expression level of gangliosides increases during retinoic acid (RA)-induced neuronal differentiation in embryonic carcinoma cells (12).…”

Section: Introductionmentioning

confidence: 99%