Changes in Global Transcriptional Profiling of Women Following Obesity Surgery Bypass

Pinhel, Marcela Augusta de Souza; Noronha, Natália Yumi; Nicoletti, Carolina Ferreira; Oliveira, Bruno A. P. de; Cortes-Oliveira, Cristiana; Pinhanelli, Vitor Caressato; Wilson, Steven E.; Machry, Ana Julia; Silva, Wilson A.; Souza, Dorotéia Rossi Silva; Marchini, Júlio Sérgio; Nonino, Carla Barbosa

doi:10.1007/s11695-017-2828-x

Cited by 24 publications

(36 citation statements)

References 37 publications

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“…Study participants. The present study included 24 normal weight women (BMI: 22.5 ± 1.6 kg/m 2 , 36.9 ± 11.8 years) and 24 severe obese women (BMI: 43.3 ± 5.7 kg/m 2 , 36.9 ± 10.2 years) submitted to RYGB which methylation and gene expression profile had been analyzed previously 20,21 . All samples from obese women were collected at preoperative time and after 6 months of bariatric surgery.…”

Section: Methodsmentioning

confidence: 99%

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Altered pathways in methylome and transcriptome longitudinal analysis of normal weight and bariatric surgery women

Nicoletti

Pinhel

Noronha

et al. 2020

Sci Rep

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DnA methylation could provide a link between environmental, genetic factors and weight control and can modify gene expression pattern. This study aimed to identify genes, which are differentially expressed and methylated depending on adiposity state by evaluating normal weight women and obese women before and after bariatric surgery (BS). We enrolled 24 normal weight (BMI: 22.5 ± 1.6 kg/m 2 ) and 24 obese women (BMI: 43.3 ± 5.7 kg/m 2 ) submitted to BS. Genome-wide methylation analysis was conducted using Infinium Human Methylation 450 BeadChip (threshold for significant CpG sites based on delta methylation level with a minimum value of 5%, a false discovery rate correction (FDR) of q < 0.05 was applied). Expression levels were measured using HumanHT-12v4 Expression BeadChip (cutoff of p ≤ 0.05 and fold change ≥2.0 was used to detect differentially expressed probes). The integrative analysis of both array data identified four genes (i.e. TPP2, PSMG6, ARL6IP1 and FAM49B) with higher methylation and lower expression level in pre-surgery women compared to normal weight women: and two genes (i.e. ZFP36L1 and USP32) that were differentially methylated after BS. These methylation changes were in promoter region and gene body. All genes are related to MAPK cascade, NIK/NF-kappaB signaling, cellular response to insulin stimulus, proteolysis and others. integrating analysis of DnA methylation and gene expression evidenced that there is a set of genes relevant to obesity that changed after BS. A gene ontology analysis showed that these genes were enriched in biological functions related to adipogenesis, orexigenic, oxidative stress and insulin metabolism pathways. Also, our results suggest that although methylation plays a role in gene silencing, the majority of effects were not correlated.DNA methylation in CpG dinucleotides is a dynamic process and best characterized epigenetic modifications 1 . Methylation patterns are established in early life and can be remodeled in adult cells, by modulating DNA interactions with proteins and transcription factors 2,3 , being able to alter gene expression profile 4 .Weight gain or loss during infancy and adulthood, by changing the energy storage and adipose tissue homeostasis, may alter molecular mechanisms and biological processes 5 . In line of this, epigenetic changes may predispose a disease risk or can occur once a disease has developed 6 . Thus, DNA methylation provides a link between environmental, genetic factors and weight control.Obesity has reached epidemic proportions and, in 2016, affected about 1.9 billion adults worldwide 7 , being associated to a public health burden. In the context of obesity treatment, bariatric surgery (BS) has been the best choice for cases of severe obesity and has been shown to be the most effective way to promote significant and sustained weight loss 8 . Among the surgical techniques, Roux-en-Y gastric bypass (RYGB) is the most performed in Brazil and worldwide, and is considered a gold standard because its high efficacy and low morbid-mortalit...

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Section: Methodsmentioning

confidence: 99%

“…p values obtained from permutations and fold change cutoff values were then used to minimize the chances of false positives. Gene expression microarray results were validated using real-time polymerase chain reaction (RT-PCR; Applied Biosystems Gene Expression Assays; Applied Biosystems, Foster City, CA, USA) 20 .…”

Section: Methodsmentioning

confidence: 99%

Altered pathways in methylome and transcriptome longitudinal analysis of normal weight and bariatric surgery women

Nicoletti

Pinhel

Noronha

et al. 2020

Sci Rep

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“…Table 3 depicts some interactions between bariatric surgery and the gene expression profile. A recent study on whole transcriptome analysis evidenced that about 1366 genes are differentially expressed (1188 upregulated and 178 downregulated genes) in the postoperative period as compared to the preoperative period of RYGB, and that these genes are associated with gene transcription, lipid and energetic metabolism, immunological processes, cell differentiation, oxidative stress, substrate oxidation, and adipocyte differentiation [ 81 ].…”

Section: Bariatric Surgery and Gene Expression Profilementioning

confidence: 99%

Bariatric Surgery and Precision Nutrition

et al. 2017

Self Cite

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This review provides a literature overview of new findings relating nutritional genomics and bariatric surgery. It also describes the importance of nutritional genomics concepts in personalized bariatric management. It includes a discussion of the potential role bariatric surgery plays in altering the three pillars of nutritional genomics: nutrigenetics, nutrigenomics, and epigenetics. We present studies that show the effect of each patient’s genetic and epigenetic variables on the response to surgical weight loss treatment. We include investigations that demonstrate the association of single nucleotide polymorphisms with obesity phenotypes and their influence on weight loss after bariatric surgery. We also present reports on how significant weight loss induced by bariatric surgery impacts telomere length, and we discuss studies on the existence of an epigenetic signature associated with surgery outcomes and specific gene methylation profile, which may help to predict weight loss after a surgical procedure. Finally, we show articles which evidence that bariatric surgery may affect expression of numerous genes involved in different metabolic pathways and consequently induce functional and taxonomic changes in gut microbial communities. The role nutritional genomics plays in responses to weight loss after bariatric surgery is evident. Better understanding of the molecular pathways involved in this process is necessary for successful weight management and maintenance.

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“…Therefore, the molecular mechanisms that govern the regulation and differentiation of adipocytes could be the major issue for better understanding of the pathological mechanism of obesity. To date, several papers have already reported the strong correlations between obesity and transcriptional changes in human adipocytes [12][13][14]. However, the full expression profile in transcriptome level that correlated to the hypertrophy and hyperplasia of adipocyte, which is important for better understanding the mechanisms of adipocyte differentiation, is still unclear.…”

Section: Introductionmentioning

confidence: 99%

A transcriptomic analysis reveals novel patterns of gene expression during 3T3-L1 adipocyte differentiation

Sun

Yang

et al. 2020

Preprint

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Background: Obesity is characterized by increased adipose tissue mass that results from increased fat cell size (hypertrophy) and number (hyperplasia). The molecular mechanisms that govern the regulation and differentiation of adipocytes play a critical role for better understanding of the pathological mechanism of obesity. However, the mechanism of adipocyte differentiation is still unclear. Objective: The present study aims to compare the gene expression changes during adipocyte differentiation in the transcriptomic level, which may help to better understand the mechanism of adipocyte differentiation. Methods: RNA sequencing technology, GO and KEGG analysis and quantitative RT-PCR menthod were used in this study. Results: A lot of genes were up- or down- regulated between each two stages of 3T3-L1 adipocyte. GO and KEGG analysis revealed that lipid metabolism and oxidation-reduction reaction were mainly involved in the whole process of adipocyte differentiation. Moreover, decreased immune response and cell cycle, adhesion were occurred in the late phase of adipocyte differentiation, which were demonstrated by divergent expression pattern analysis. In addition, quantitative RT-PCR results demonstrated that the mRNA expression level of Trpv4 , Trpm4 , Trpm5 and Trpm7 were significantly decreased in the differentiated adipocytes. On the other hand, the mRNA expression level of Trpv1 , Trpv2 , Trpv6 and Trpc1 were significantly increased. Conclusions: These data presents the description of transcription profile changes in adipocyte differentiation and provides an in-depth analysis of the possible mechanisms of adipocyte differentiation. These data offer new insight into the understanding of the mechanisms of adipocyte differentiation.

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Changes in Global Transcriptional Profiling of Women Following Obesity Surgery Bypass

Cited by 24 publications

References 37 publications

Altered pathways in methylome and transcriptome longitudinal analysis of normal weight and bariatric surgery women

Altered pathways in methylome and transcriptome longitudinal analysis of normal weight and bariatric surgery women

Bariatric Surgery and Precision Nutrition

A transcriptomic analysis reveals novel patterns of gene expression during 3T3-L1 adipocyte differentiation

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